FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13780226
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3120362.2643750545165227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC6450.061.762433
CGCACTT6950.059.485445
ACGCACT7800.054.801414
GCCGCCC5600.052.052544145
CGTCGCC3550.049.266636145
AGCGGCC6450.046.32273145
GCGTCGG2900.045.23187145
TAGTAAC13200.043.3199922
AAACACG8100.042.9810335
GTAGGGG3350.041.331276145
CACGAGC6050.040.9011888
TCTCGGG3400.040.72346145
ACGGCCC4150.040.38776145
GTCGCCC4550.040.04042145
TCAAGCC9400.039.537785145
CGCGTCC3150.039.32859145
TCGCCGG4650.039.179333145
AGCGCCC4650.039.179333145
GCACGCA10400.038.9753382
GTTCGCC2250.038.865902145
Produced by FastQC (version 0.11.5)