FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_PLASMID_PB2_PR8_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_PLASMID_PB2_PR8_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATACAAGGGAAAACAACTTAAAACAAATGCTGAAGACATAGGAACCAA40.2597402597402597No Hit
ATGCTACACTGCTAACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG30.19480519480519481No Hit
GTAAGAAATACAGAAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA30.19480519480519481No Hit
CTATAGAACATGCATGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT30.19480519480519481No Hit
CTTTACATACAGTACCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG30.19480519480519481No Hit
ATGTTAAACCATCACCTGTGGCCCATTTGGCATAATTGGTTTTCTGTAAC20.12987012987012986No Hit
GAGCTAAGGATCTGTGGACCATCCATGCTATGGTTCTTGGCATTCCCTCA20.12987012987012986No Hit
CTGGATATCCTGTGTTTTACTTTCATTGGTATTCTCTTTATGAGAAAACC20.12987012987012986No Hit
GTATTGTCTCCTGATACTGTCATGCTGATCCCTCCTGGTGGGCAATTACT20.12987012987012986No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC20.12987012987012986No Hit
GTATTCCACTCTGAATATCCTGTCTTTTACTTTCATTGGTATTCTCTTTA20.12987012987012986No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT20.12987012987012986No Hit
GCACTATATCTTTGCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG20.12987012987012986No Hit
ATCAATGGGTGCTAAAAAATTTGGTAACACTAAAGGCTCAGTTTCTTCTA20.12987012987012986No Hit
CCTTAAGATTCGAGAGGGCCGAATAATAAAAGAAATATTTCCAACAGGAA20.12987012987012986No Hit
ATTCTCTACCATCATGGCATGCGGACCCGCTCCAAGCTGTCATGTGGAAA20.12987012987012986No Hit
CTGCTATGAGCTCAGTGTGAATGTGATGCTTGTTTTTTCGCACAAAGCAC20.12987012987012986No Hit
GTATGTTTTACTCATCCTGTTGGAAATATTTCTTTTATTATTCGGCCCTC20.12987012987012986No Hit
CTCCAATGTTGCTATGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT20.12987012987012986No Hit
CTTTTTTAATATTACCCTGCATTCACCACATCTCACATGGAACTCCTCTT20.12987012987012986No Hit
AAATTACACTGTTCGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT20.12987012987012986No Hit
ATACTGAGGAATGAACATGCGGATTTGCCAGTAACAAAACCATAGAATGT20.12987012987012986No Hit
GTGCTGGGGTTATATCTGCTTCTTTGATGCCTTCAATAGACAAATGTCCT20.12987012987012986No Hit
GCTTTAGCACTTCAATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA20.12987012987012986No Hit
GTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATAGCAGA20.12987012987012986No Hit
CTCTTGGTATTCCAGCTTCTTTAGGGAACAATATTTCCATTATCACATTA20.12987012987012986No Hit
TCCTAATGCAGCTATTATTATGTCACAGGCGACATCACCTCCGTCTATAG20.12987012987012986No Hit
CTTCAATACTATTGCTGGGATATGTAAGTTTCTCAAATTGTAAATGTTGG20.12987012987012986No Hit
ATGCATTACTGTCAGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA20.12987012987012986No Hit
CTACTATATTGTCACATGGTCATGTACCTGAATCCTGGAAATTATTCAAT20.12987012987012986No Hit
ACACAATAGACACAGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA20.12987012987012986No Hit
CCATTATGCAGCTAAACCAGGGGGATACTACAATGGAACAAGAGAAGACA20.12987012987012986No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT20.12987012987012986No Hit
GATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCAAGTGCCT20.12987012987012986No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT20.12987012987012986No Hit
GTAGAAAGATAATAAGAAGATCAATAGTCGCATCAAACCCATTGGAGCTA20.12987012987012986No Hit
CTCTACCATCATGCCATGCGGACCCGCTCCAAGCTGTCATGTGGAAAATA20.12987012987012986No Hit
TGCCAGTACAATGAGAGTTATCATCGTTCCTTTCAATTTTTCTCTTTTTT20.12987012987012986No Hit
CATTGGAAACAAGAAATACTTATGGGATGGACTGCAATCTTCCGATGATT20.12987012987012986No Hit
TAACAGGGCCTTGAGAATCTGATGGGAACGAAGGGAGTGGAGGCATCAAG20.12987012987012986No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT20.12987012987012986No Hit
ATATAGTAGCGCTAAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT20.12987012987012986No Hit
GCATTAGGATTAAAGATCAGACAAAGACAAAGATTTGGACGACTTGAACT20.12987012987012986No Hit
GAACAGACTCAACAATTCCTCCATTACAGAGTCATATTAACACCTGTGAC20.12987012987012986No Hit
ATATTACTCTTAGAGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT20.12987012987012986No Hit
CTGTGACAGTGTCGCACAGCAGTTGTCCTGAGCCCATTAAACAGTAAATG20.12987012987012986No Hit
ACATAGACAATATAGACCCGAAAGGAGCAATAGAGAGAAACCTAGCAAGG20.12987012987012986No Hit
GCCTTGGAATGGAAAAAAAACAAAAGATGCTTAACTGACATACAGAAAGC20.12987012987012986No Hit
GTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCTAAAATTTT20.12987012987012986No Hit
GTTTAGAACAGACACAACCATTCCTCCATTACAGAGTCATATTAACACCT20.12987012987012986No Hit
GTCATAATGGGAGACAATGACGTAAGTGAATTAGAATCACAAGCACAACT20.12987012987012986No Hit
CAATTTTCCCTTTGAATGACATCATTCTGCCGCATATAGTTAGGACTTCT20.12987012987012986No Hit
GGGGAAGTCATAAGGGGAGCCAATGACGTAAGTGAATTAGAATCACAAGC20.12987012987012986No Hit
GACTTATTTGGACGCCCCCAGACCAAATGATGGAAGCATAACAGGGCCTT20.12987012987012986No Hit
ATTACATCCTTTGAAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA20.12987012987012986No Hit
GTTCATAGCTGAGTCCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG20.12987012987012986No Hit
GTGTTATTATCACAATATGTGTCAGCTTCATTGTCATACTTACTATATTC20.12987012987012986No Hit
TTCTAATGCTGTAGATGCTTTTCCTTCTTCGTCAAGAAAATTCATGTCAC20.12987012987012986No Hit
AAGACTTATTTGGGCACCCCCAGACCAAATGATGGAAGCATAACAGGGCC20.12987012987012986No Hit
GATATATCCCTTAATCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC20.12987012987012986No Hit
GTCTTGATTGTAGATGAATATCATTAGTTCCATGCTATTTCCCAGTTTCT20.12987012987012986No Hit
CTTCTGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC20.12987012987012986No Hit
CATATGGACCAATAGGAGATACTGAAGGTTTCGAAAAAGTCTACGAAAGC20.12987012987012986No Hit
GTCCAATCGAAAGACTGTCTCCCCTGGGTTAATTTGTCTACAGTTGTGAC20.12987012987012986No Hit
ACTTTGTATTTTCTCATGCTGGCATTGCTTTCATTCAGCAATGAAGTGTG20.12987012987012986No Hit
GTAATAAGTGACAGGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC20.12987012987012986No Hit
GCTCATAGCAGAGAAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT20.12987012987012986No Hit
CTACAGGCACATTGTATGGTTTTGTTACTGGCAAATCCGCATGTGCATTC20.12987012987012986No Hit
TATCAAGGGAGCTAAGTTTCCTTTGGTTTCTCCGAATCTATGGGGGCTAA20.12987012987012986No Hit
TATATCACCCGGGAGGGAATAAACTAACTGAATCTAGGTCCCAATCGATG20.12987012987012986No Hit
CTCCATTACAGAGACATATTAACACCTGTGACAGTGTCCCACAGCAGTTG20.12987012987012986No Hit
ATGTATGCCAGTACAATGGGAGTTATCATCGTTCCTTTCAATTTTTCTCT20.12987012987012986No Hit
CTCCTAATTAGCCCCCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC20.12987012987012986No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT20.12987012987012986No Hit
ATATTCGGATATAATGCTAAAATTTTCACGAACAGAAGTAACTGCACCAA20.12987012987012986No Hit
ACATAGACAATATGGACCCGAAAGGAGCAATAGAGAGAAACCTAGCAAGG20.12987012987012986No Hit
GTCCATATGTATTCCACCAGGTGACTGCTGCTATTGAGCACATTTGGCCT20.12987012987012986No Hit
ATATTGCAGAGTGAATGGCACAAATAAGATCCAAATGAAATGGGGAATGG20.12987012987012986No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)