FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_DEMUX_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_DEMUX_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAAGAACATTGGAAACAAAGAATACTTATGGGATGGACTGCAATCT11.4285714285714286No Hit
GCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGA11.4285714285714286No Hit
ATCCTTGTGATCCTCCCTCCAATTTCAATGTACTGTAGCTCTGCTCACAC11.4285714285714286No Hit
AAACACGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCTCAGG11.4285714285714286No Hit
GTTACGCACTCTTTAAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTG11.4285714285714286No Hit
CTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAAATCCCTT11.4285714285714286No Hit
GCATTGGAGTAACAGTGATAAAGAACAACATGATAAATAATGACCTTGGA11.4285714285714286No Hit
AAGCCCTGTCAGTCCCACCGAAATTTGCCAAAGACCCAGACACGAAGCAA11.4285714285714286No Hit
CCGTTGCATGGGATACCGTGCGTGATGTGGCGGCGCCCGTCAGGATGGCG11.4285714285714286No Hit
ATATCGTCATTCCTCACTCCTCCACCTGGAGTGTACATTTGTTCCCAACA11.4285714285714286No Hit
GTAGAAACACGAGCTATTTCTCAGTTTGATTAGGCTTTCACCCCTACCCT11.4285714285714286No Hit
GGTACAACATGAAAGCTGTAATAAAATTTACTGTAAACCATCCATGAAGG11.4285714285714286No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC11.4285714285714286No Hit
TCTACGCTCCAGTCCTCGCTCACCGGGCCCGGTGAGCGAGGACTGCAGCG11.4285714285714286No Hit
GTGTTCACGCTCACCGTGCCCAGTGAGCGTGAAAACAAACCCTAAAATCC11.4285714285714286No Hit
TCATCTCTATGGGACCAATGACCACCTTCAACGGTGCCTTCACCCCTGCG11.4285714285714286No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCAAGAAAATT11.4285714285714286No Hit
ACCTTGAAAAGGCCAATAAGATTAAATCTGAGAACACACACATTCACATT11.4285714285714286No Hit
GCTTACAAGCGGTCGGAGCTGGCAGGTCCAGTGACGGCGTGCCTTTTGCA11.4285714285714286No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAAAGCTCATA11.4285714285714286No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG11.4285714285714286No Hit
ATGCAAAAGGCACGCCGTCACTGGACCTGCCAGCTCCGACCGCTTGTAAG11.4285714285714286No Hit
ATGCAACATACCAGAGGACAAGAGCTCTTGTTCGGACTGGAATGGATCCC11.4285714285714286No Hit
GGTGACGTTATTGTTTCGAATGGTGGATCTGGCAATCAACGTTGCGACAT11.4285714285714286No Hit
ACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGT11.4285714285714286No Hit
GACTATGCGCCCTATTAAGACTCGCTTTCGCTTCGGCTACGTGGCTGAAC11.4285714285714286No Hit
GTACAGCAAAGCTTCGGCGGCGCTGATAGAGAAGGTAAACAGACTTACAA11.4285714285714286No Hit
CCCCAGTCCTTTTTATTAAACTAAGATTTTTTGTTATAGATACTTTTTCT11.4285714285714286No Hit
ACTTTGGATAGACACAGCTATGGTAAAAGAGGAAAGCCACTCGTACGTCA11.4285714285714286No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATTTTAAAAGAGGAATCCAGC11.4285714285714286No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACCATGGATTGGGTGAT11.4285714285714286No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT11.4285714285714286No Hit
ATTAGATTGCATTCTGTGAGGCTGCTTGAAACAGACCTGGATGTTCCTCA11.4285714285714286No Hit
GTACTTAAACAAATGAGAGACCAAGAGGTCATGAAAACTGACCAGTTCAT11.4285714285714286No Hit
ATGTTGACAAAATGACCATCGTCAACATAGAGCCTGTCTCTTATACACAT11.4285714285714286No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC11.4285714285714286No Hit
GTAGTAACAAGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCT11.4285714285714286No Hit
ACTTCCACTGTTTGTGGGTTCGTTGCATTTTTGTAGTTGTCCTTTGGAAC11.4285714285714286No Hit
CTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCCATCAACT11.4285714285714286No Hit
GGCCGTGACAGGTGTGAACATTATATGTAACACGACCGGGATAGCTAAAC11.4285714285714286No Hit
GGAATGGACATATATCGGAGGTAATGGCCCTGACAATAATGCATTTCGCA11.4285714285714286No Hit
GATGGATTCATAAATAGGAGTACTTTCATCATTTTTACTCCAATTGCTTT11.4285714285714286No Hit
GCTCCACGAAGCCCGGTGATAGCCCTGTACACGTATAGGATGCGAGCATA11.4285714285714286No Hit
CCCAGCACTCTGCTGCTCCAGATGAGAATCCGCACCCATGGCAGCAGGCA11.4285714285714286No Hit
GTCTGTTATTATGCCATTGTACTTTAACACAGCCACTGCCCCACTGTCTG11.4285714285714286No Hit
CTTCGGAGCTATTGCTGGTTTTAAAGAAGGAGGATGGGAAGGAATGATTG11.4285714285714286No Hit
CCTATGTATCCAAGTAGATTCTCTTGGTATTCCTGCTTCTTTAGGGAATA11.4285714285714286No Hit
GGGAAAGGCGAAAAGAACCTCGAACAGAGGAGTGAAAAGAACCTGAAACC11.4285714285714286No Hit
GTCCATGGGTCTCCATCATACTTTACATACAGTCCCATCCCCATTCTTTG11.4285714285714286No Hit
GAATATCATCAGTTCCATGCTATTTCCCAGCTTCTCTTTCTTTTTCCAAA11.4285714285714286No Hit
GCATAGTTTTCCGTTATGCTTGTCTTCCAGAAGATTAACAGAGTGTGTTA11.4285714285714286No Hit
TATTTTCTCTGCTTCCTTCCCTTTAATCTACCAATTACTAAGTCCCATGT11.4285714285714286No Hit
AAGCAGAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCC11.4285714285714286No Hit
GCCATTGGTAACCACCCTAGTATCCGTACTGGTATACGCGATGATCATCC11.4285714285714286No Hit
AGACTACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAGCAATG11.4285714285714286No Hit
CGCCGCTGCGACGCCCGCCGCCGAGACGTGCGCGCGGCGACGCCCGCGGC11.4285714285714286No Hit
GTAGAAGGGCAACTTTCCAAACCGTATTTAAAGCGACGATAAATGAATGT11.4285714285714286No Hit
GCATATACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC11.4285714285714286No Hit
TGTGATCATAGCTCACTGTAACCTCGAACTCCTGGGCTCCAGCCATAGGA11.4285714285714286No Hit
GGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTG11.4285714285714286No Hit
GCGCTTAGCTTTCATGGCCTTCTGCTCTTTCAAACGCTTCATGACAGCTC11.4285714285714286No Hit
GCCTACGAGTTACTCTTGTCTGGCAAGGTTAAGTGGTTCAGCCACGTATC11.4285714285714286No Hit
TGTTATAGTTCAGTATTCTAACCAATTTCGAAACGACGGGGTCTCCGTGC11.4285714285714286No Hit
GACTCAAATCCTCAAAAGTTCACCTCATCTGCTAATTGAGTGACCCCACA11.4285714285714286No Hit
CCACTACATGTTACTGCAACTTCAACCTGCCCAAGGGTAGATCACAAGGT11.4285714285714286No Hit
TAATCCCTGCAACGTTAAATGCTGCCGGCAAGGGAAACCACACAATCGGG11.4285714285714286No Hit
CGGATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAATG11.4285714285714286No Hit
TATGATACCTGTGCTGGGCATCAGCAATCAGCTCACAAGTTGCACACTCC11.4285714285714286No Hit
CAAGTGCCTACATCCTTACCTCGCGCTACATCTCAGACATGATTGTTGAG11.4285714285714286No Hit
GGATGTTCCTCATCCATTCTATCCAAAGCCTCCAGAACGCAATCTAATTG11.4285714285714286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAT50.0145.04
CAATGGC50.0145.07
ATGGCAG50.0145.09
TACAATG50.0145.05
GACAAGG50.0145.0145
AATGGCA50.0145.08
AGACTAC50.0145.01
ACAATGG50.0145.06
ACTACAA50.0145.03
GACTACA50.0145.02
Produced by FastQC (version 0.11.5)