FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_DEMUX_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_DEMUX_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAATTTTCTTAATCGA21.5873015873015872No Hit
CTCTCTCCTACACCTAAAATGTTGCAATTACGAACCCACCTACACCTGTT10.7936507936507936No Hit
GCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGA10.7936507936507936No Hit
AAACACGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCTCAGG10.7936507936507936No Hit
GAGAACAACACACTGAGGAATGCACATGCGGCTTTGCCAGCAATAAAACC10.7936507936507936No Hit
GTAGAAACACGAGCTATTTCTCAGTTTGATTAGGCTTTCACCCCTACCCT10.7936507936507936No Hit
GACTCTACATAACGGACTGGCTTCCTCCAAACCCTGCCCCCTCCTAGTTG10.7936507936507936No Hit
GCACATATAAATCAAATACAAAGAAGCGAAAACATGAAAATAAGAATAAA10.7936507936507936No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA10.7936507936507936No Hit
ACTCCTGTCTGGCAAGGTTAAGTGGTTCAGCCACGTATCCGAAGCGAAAG10.7936507936507936No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC10.7936507936507936No Hit
TCTACGCTCCAGTCCTCGCTCACCGGGCCCGGTGAGCGAGGACTGCAGCG10.7936507936507936No Hit
GCATGGAGCGGTGTCGCGTGACAGGAGGGTAAGGAATAGACATATAAAGG10.7936507936507936No Hit
CCGCTGCTGACCTCGCTCCCTGGGCCCCATGAGCGTGCCAAGCACCCAGC10.7936507936507936No Hit
GTGTTCACGCTCACCGTGCCCAGTGAGCGTGAAAACAAACCCTAAAATCC10.7936507936507936No Hit
TCATCTCTATGGGACCAATGACCACCTTCAACGGTGCCTTCACCCCTGCG10.7936507936507936No Hit
ACCTTGAAAAGGCCAATAAGATTAAATCTGAGAACACACACATTCACATT10.7936507936507936No Hit
CCATTTTCGACAACATCATAAGTATGTACTGAAAGAGAAGGTAATGGACA10.7936507936507936No Hit
GCTTACAAGCGGTCGGAGCTGGCAGGTCCAGTGACGGCGTGCCTTTTGCA10.7936507936507936No Hit
CTATTAAGACTCGCTTTCGCTTCGGATACGTGGCTGAACCACTTAACCTT10.7936507936507936No Hit
TCCTATTTCCATTACTGGTCTAAGAGATGCACTTCGGGCCAATTTGCAGA10.7936507936507936No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAAAGCTCATA10.7936507936507936No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG10.7936507936507936No Hit
ATGCAAAAGGCACGCCGTCACTGGACCTGCCAGCTCCGACCGCTTGTAAG10.7936507936507936No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA10.7936507936507936No Hit
GGTGACGTTATTGTTTCGAATGGTGGATCTGGCAATCAACGTTGCGACAT10.7936507936507936No Hit
ACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGT10.7936507936507936No Hit
GTACAGCAAAGCTTCGGCGGCGCTGATAGAGAAGGTAAACAGACTTACAA10.7936507936507936No Hit
CCCCAGTCCTTTTTATTAAACTAAGATTTTTTGTTATAGATACTTTTTCT10.7936507936507936No Hit
TCTTTGACTAAGTAGTTGCAGACTTGGCTAAGAACTTCTGTGATCATCTA10.7936507936507936No Hit
GAAAAAGACAGTCTTTCACTTGGCGAGCTTCCAACCCCTCATGTCGATAT10.7936507936507936No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATTTTAAAAGAGGAATCCAGC10.7936507936507936No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACCATGGATTGGGTGAT10.7936507936507936No Hit
GTACTTAAACAAATGAGAGACCAAGAGGTCATGAAAACTGACCAGTTCAT10.7936507936507936No Hit
AGTTGAAAGAGTGAGAAAAAGAGTACTACTAAACCCTCTCACCAAGGAAA10.7936507936507936No Hit
ATGTTGACAAAATGACCATCGTCAACATAGAGCCTGTCTCTTATACACAT10.7936507936507936No Hit
CGGAGTGACCACACATTACGTTTCACAGATTGGTAGCTTCCTAAATCAAA10.7936507936507936No Hit
CTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCCATCAACT10.7936507936507936No Hit
GGCCGTGACAGGTGTGAACATTATATGTAACACGACCGGGATAGCTAAAC10.7936507936507936No Hit
GGAATGGACATATATCGGAGGTAATGGCCCTGACAATAATGCATTTCGCA10.7936507936507936No Hit
GATGGATTCATAAATAGGAGTACTTTCATCATTTTTACTCCAATTGCTTT10.7936507936507936No Hit
AATTTGTCTACAGTTGTGACCATTAGGGCCTCCATAGCATTCTGTGAGGC10.7936507936507936No Hit
GCTCCACGAAGCCCGGTGATAGCCCTGTACACGTATAGGATGCGAGCATA10.7936507936507936No Hit
ACCAGGGGGATACTACAATGGAACAAGAGGAGACAGAAACAAGCTGAGGC10.7936507936507936No Hit
GGGAAAGGCGAAAAGAACCTCGAACAGAGGAGTGAAAAGAACCTGAAACC10.7936507936507936No Hit
GTCCATGGGTCTCCATCATACTTTACATACAGTCCCATCCCCATTCTTTG10.7936507936507936No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT10.7936507936507936No Hit
GAATGTTAAAACGGGTCGAGTCTCTGCAAATATATAGAGCTTCGATTGGA10.7936507936507936No Hit
GCATAGTTTTCCGTTATGCTTGTCTTCCAGAAGATTAACAGAGTGTGTTA10.7936507936507936No Hit
GTTGTTGATTACATGGTGCAAAAATCTGGAAAAACAGGAACAATTACCTA10.7936507936507936No Hit
AAGCAGAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCC10.7936507936507936No Hit
AGACTACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAGCAATG10.7936507936507936No Hit
GCATATACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC10.7936507936507936No Hit
CTCAAAGCACTAATTGGTCCCTCTAGAAGCTTTTAAAACCCAAAGTACAG10.7936507936507936No Hit
TGTGATCATAGCTCACTGTAACCTCGAACTCCTGGGCTCCAGCCATAGGA10.7936507936507936No Hit
TATACAACGACATCCTCGAACAATCTTACCGGCAGCCGAAAGGGTAATCA10.7936507936507936No Hit
TACCTCATCTGACTATGCGCCCTATTAAGACTCGCTTTCGCTTCGGATAC10.7936507936507936No Hit
ATTTCTACTCTCACCCCCAGGCACACCCTAGTGTCTTGTGCAAATACCAT10.7936507936507936No Hit
GCCTACGAGTTACTCTTGTCTGGCAAGGTTAAGTGGTTCAGCCACGTATC10.7936507936507936No Hit
TGTTATAGTTCAGTATTCTAACCAATTTCGAAACGACGGGGTCTCCGTGC10.7936507936507936No Hit
GACTCAAATCCTCAAAAGTTCACCTCATCTGCTAATTGAGTGACCCCACA10.7936507936507936No Hit
CGGATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAATG10.7936507936507936No Hit
TATGATACCTGTGCTGGGCATCAGCAATCAGCTCACAAGTTGCACACTCC10.7936507936507936No Hit
CAAGTGCCTACATCCTTACCTCGCGCTACATCTCAGACATGATTGTTGAG10.7936507936507936No Hit
GTATCAAGAACATTGGAAACAAAGAATACTTATGGGATGGACTGCAATCT10.7936507936507936No Hit
ATCCTTGTGATCCTCCCTCCAATTTCAATGTACTGTAGCTCTGCTCACAC10.7936507936507936No Hit
GTTACGCACTCTTTAAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTG10.7936507936507936No Hit
CTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAAATCCCTT10.7936507936507936No Hit
GCATTGGAGTAACAGTGATAAAGAACAACATGATAAATAATGACCTTGGA10.7936507936507936No Hit
GTCTGTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTGTCCC10.7936507936507936No Hit
AAGCCCTGTCAGTCCCACCGAAATTTGCCAAAGACCCAGACACGAAGCAA10.7936507936507936No Hit
CCGTTGCATGGGATACCGTGCGTGATGTGGCGGCGCCCGTCAGGATGGCG10.7936507936507936No Hit
ATATCGTCATTCCTCACTCCTCCACCTGGAGTGTACATTTGTTCCCAACA10.7936507936507936No Hit
GCACCATCTGATAAGGCTAAATACTCCTGAGAGACCGATAGTGAACTAGT10.7936507936507936No Hit
GGTACAACATGAAAGCTGTAATAAAATTTACTGTAAACCATCCATGAAGG10.7936507936507936No Hit
CAAATGGACCATTACCCGAAGATAATGAGCCGAGTGCCTATGCGCAATTA10.7936507936507936No Hit
ATGCTATGGAGGCCCTAATGGTCACAACTGTAGACAAATTAACCCAGGGG10.7936507936507936No Hit
CTCTCAGCCAGAATCCGGACCTTCTTTTTTGTTGCTGTTGTTCCCTTACG10.7936507936507936No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCAAGAAAATT10.7936507936507936No Hit
GATCACAAGGTTTCGCGTCTACCTCATCTGACTATGCGCCCTATTAAGAC10.7936507936507936No Hit
GGATTTGACTCCCCCGCACCCAATCGGTAGCTCTACCTCTATAAGACTCC10.7936507936507936No Hit
CCGTGTGCTTACAAGCGGTCGGAGCTGGCAGGTCCAGTGACGGCGTGCCT10.7936507936507936No Hit
CTGCCCACCAGGAGGGATTAGCATGACAGTAACAGGAGACAATACAAAAT10.7936507936507936No Hit
CCAGACAAGAGTAACTCGTAGGCTCATTATGCAAAAGGCACGCCGTCACT10.7936507936507936No Hit
ATGCAGATCGAATCTGCACTGGGATAACATCGTCAAACTCACCACATGTC10.7936507936507936No Hit
ATCTCTTACTCACAACCAAACCTCTACATGGCATCGCTCCCTCACCCCCT10.7936507936507936No Hit
AGCACAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCT10.7936507936507936No Hit
ATGCAACATACCAGAGGACAAGAGCTCTTGTTCGGACTGGAATGGATCCC10.7936507936507936No Hit
GACTATGCGCCCTATTAAGACTCGCTTTCGCTTCGGCTACGTGGCTGAAC10.7936507936507936No Hit
ACTTTGGATAGACACAGCTATGGTAAAAGAGGAAAGCCACTCGTACGTCA10.7936507936507936No Hit
CACGGTACTAGTTCACTATCGGTCTCTCAGGAGTATTTAGCCTTATCAGA10.7936507936507936No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT10.7936507936507936No Hit
ATTAGATTGCATTCTGTGAGGCTGCTTGAAACAGACCTGGATGTTCCTCA10.7936507936507936No Hit
GGTAATGTTAGGGCAAGACCCTGAGGTTCCAATTTTGTAGGGTCCTCCTG10.7936507936507936No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA10.7936507936507936No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC10.7936507936507936No Hit
GTAGTAACAAGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCT10.7936507936507936No Hit
ACTTCCACTGTTTGTGGGTTCGTTGCATTTTTGTAGTTGTCCTTTGGAAC10.7936507936507936No Hit
GACATACATATTTCAGTATGAATCCATAGCTTCGGTAATACACTTGATGC10.7936507936507936No Hit
CCCAGCACTCTGCTGCTCCAGATGAGAATCCGCACCCATGGCAGCAGGCA10.7936507936507936No Hit
ACATGATTCTTGAGGAACAATGCTACGCTAAATGTTGCAACCTATTTGAG10.7936507936507936No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC10.7936507936507936No Hit
GTCTGTTATTATGCCATTGTACTTTAACACAGCCACTGCCCCACTGTCTG10.7936507936507936No Hit
CCTTTCACCCCTACCCTCAGGTCATCCGGAAACTTTTCAACGTTTATCGG10.7936507936507936No Hit
CTTCGGAGCTATTGCTGGTTTTAAAGAAGGAGGATGGGAAGGAATGATTG10.7936507936507936No Hit
CCTATGTATCCAAGTAGATTCTCTTGGTATTCCTGCTTCTTTAGGGAATA10.7936507936507936No Hit
GAATATCATCAGTTCCATGCTATTTCCCAGCTTCTCTTTCTTTTTCCAAA10.7936507936507936No Hit
CGTATAGATTGCTCGCCTAGCAGTACCCTGAGTACCGCGTGGTCGGAGAC10.7936507936507936No Hit
CGCATAGTCAGATGAGGTAGACGCGAAACCTTGTGATCTACCCTTGGGCA10.7936507936507936No Hit
TATTTTCTCTGCTTCCTTCCCTTTAATCTACCAATTACTAAGTCCCATGT10.7936507936507936No Hit
GCCATTGGTAACCACCCTAGTATCCGTACTGGTATACGCGATGATCATCC10.7936507936507936No Hit
CGCCGCTGCGACGCCCGCCGCCGAGACGTGCGCGCGGCGACGCCCGCGGC10.7936507936507936No Hit
GTAGAAGGGCAACTTTCCAAACCGTATTTAAAGCGACGATAAATGAATGT10.7936507936507936No Hit
ACATCCACTTACAGGGGAATCACCAAGTCCGTACCCTAGTCCACTCCACG10.7936507936507936No Hit
GGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTG10.7936507936507936No Hit
CAAAGTGGTAGAATTGTTGTTGATTACATGGTGCAAAAATCTGGGAAAAC10.7936507936507936No Hit
AACACGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCTCAGGT10.7936507936507936No Hit
AAACTCACCACATGTCGTCAAAACTGCTACTCAAGGGGAGGTCAATGTGA10.7936507936507936No Hit
GCGCTTAGCTTTCATGGCCTTCTGCTCTTTCAAACGCTTCATGACAGCTC10.7936507936507936No Hit
ATCAACCACATTTCCAACCTAAGATAGGACTGTTTCGCAGGACACGTTGG10.7936507936507936No Hit
CCACTACATGTTACTGCAACTTCAACCTGCCCAAGGGTAGATCACAAGGT10.7936507936507936No Hit
AGATAGCTATCATCAGTGTTACGGCCAAACTGGAGGCAGCAGTTGAGTAG10.7936507936507936No Hit
TAATCCCTGCAACGTTAAATGCTGCCGGCAAGGGAAACCACACAATCGGG10.7936507936507936No Hit
GGGTAGATCACAAGGTTTCGCGTCTACCTCATCTGACTATGCGCCCTATT10.7936507936507936No Hit
GGATGTTCCTCATCCATTCTATCCAAAGCCTCCAGAACGCAATCTAATTG10.7936507936507936No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers