FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9971_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9971_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460941
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA61420.42041396606707593No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT49860.3412868829062912No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT48970.3351949188913173No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT44350.30357146524055384No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT42360.2899501074992077No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA40090.27441217680933044No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGTGATTCTTCTACCTCCAAA39910.2731800941995604No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC35960.24614272581849642No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC34290.23471173716118587No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG33860.23176842870451306No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG33620.2301256518914864No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT31890.21828396903091912No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA30680.21000163593190962No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT29300.2005556692570063No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT28600.19576423688567848No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT27750.189946069006209No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA26590.18200598107658011No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24720.16920601174174726No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC22970.1572274308134278No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA22790.15599534820365776No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG22320.15277824361148054No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT21950.15024562935806443No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC21480.14702852476588718No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT21460.14689162669813496No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG21320.14593334022386942No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG21250.14545419698673662No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA20930.14326382790270106No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT20930.14326382790270106No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA20740.14196329625905496No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA20120.13771945615873604No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT20090.13751410905710773No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT20060.1373087619554794No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC19820.1356659851424527No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC19630.1343654534988066No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG19630.1343654534988066No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA19130.130943001805001No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT18340.1255355281287882No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA17620.1206071976897082No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17130.11725319502977877No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17010.11643180662326542No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT16850.11533662208124765No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA16420.11239331362457486No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG16190.1108189858454243No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG16070.10999759743891095No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT15880.10869706579526484No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA15710.10753343221937095No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAGAA15670.1072596360838665No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC15650.10712273801611427No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA15270.10452167472882203No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC15110.10342649018680425No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA14850.10164681530602537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCC351.0913936E-11145.10722145
GCGAGAA151.2265037E-4145.10721145
TGACGCC600.0133.01495145
ATAAGCC650.0122.78302145
ACACGTG750.0115.91897
GGAGCGT256.4884007E-6115.91896
GAGCAGA1200.0114.707481
AACACGT700.0113.8489156
CGCACCC501.9463187E-10101.57505145
AAACACG2300.0100.795595
CACCCCC301.5938462E-596.738144145
GGCACCC451.0864824E-896.73814145
CGGTGCA301.6055457E-596.595772
CGGAGCG301.6055457E-596.595775
GAAACAC2600.094.738164
ACGAGCC1050.089.702229
AAGCGGA900.088.546132
AGCCACC259.3600946E-487.06432145
CCAAACC259.3600946E-487.06432145
CTAGGCC259.3600946E-487.06432145
Produced by FastQC (version 0.11.5)