FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9932_2_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9932_2_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387082
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA110700.7980782679034116No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT95200.6863328916387063No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT90460.652160434639048No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA82310.5934039948611546No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT80150.5778317359752343No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC68220.4918238431469805No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG64460.4647165776788972No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT61760.4452512540714969No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC61730.44503497269808134No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA60780.4381860625399219No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG60190.4339325288627493No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA55750.40192288559724665No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT54550.3932716306606242No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT50110.36126198739512155No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA44780.3228359967182906No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT44700.32225924638918246No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA43450.31324752249686755No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC43310.3122382094209283No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT42650.30748001920578594No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG41730.30084739042104214No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC41540.2994776083894103No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG40630.292917073395805No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA39480.2846262874148753No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA39270.28311231780096635No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT38320.276263407642807No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT37130.26768424649732314No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG34240.246849140858291No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA32910.23726066663686793No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC32910.23726066663686793No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT32660.23545832185840493No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG32400.2335838832888034No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA31850.22961872477618483No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG31700.22853731790910703No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31480.2269512545040596No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG31310.2257256600547048No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT31050.22385122148510325No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA29140.21008130737764602No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT28780.20748593089665932No Hit
GTTGTTCCCATTCCCGATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTC28280.20388124133973334No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG28210.20337658480176368No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27220.19623929947905025No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC26400.19032760860569164No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA25320.18254147916273153No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT25090.18088332196654558No Hit
CTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGA24560.17706235103620407No Hit
CTCCTGGCACTGAGGATCTCGCTGCTCTGCTATGAGCTCTGTGTGAATGT24560.17706235103620407No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA24470.1764135069159574No Hit
GTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTG24380.1757646627957107No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC24020.173169286314724No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT23990.17295300494130844No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA23970.1728088173590314No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG23590.17006925329576766No Hit
CCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA23580.16999715950462915No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC23570.16992506571349061No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT23500.16942040917552098No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC22940.1653831568717639No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT22670.16343662451102386No Hit
AGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC22640.1632203431376083No Hit
CCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT22500.16221103006166904No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC22480.162066842479392No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT22360.16120171698572977No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT22170.1598319349540979No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAA21540.15529002611237114No Hit
GCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTC21420.15442490061870892No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA21370.1540644316630163No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG21320.1537039627073237No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA21280.15341558754276965No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA20790.1498829917769822No Hit
GTTTGGAGACACAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAG20770.14973880419470514No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT20530.14800855320738068No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA19410.1399340485998665No Hit
CTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAG19290.13906892310620425No Hit
CCCAAAGACCAGGAAAGAAAAAGAAGATTCATCACAGAGCCCTTATCGGG19170.138203797612542No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA19010.13705029695432572No Hit
GTTTTTGCTGTGTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCC18910.1363293590429405No Hit
CTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATAG18870.13604098387838642No Hit
GCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGGATCTCGC18550.1337339825619538No Hit
TCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCATCTGC18400.132652575694876No Hit
GTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGATA17970.12955254267591967No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT17960.12948044888478116No Hit
CATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGG17930.1292641675113656No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC17530.1263804158658248No Hit
GAAGATGGAGAAGGCAAAGCAGAATTAGCAGAAAAATTACACTGTTGGTT17400.12544319658102404No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG16930.12205478839751363No Hit
ACTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACA16800.12111756911271288No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA16770.12090128773929731No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT16530.11917103675197285No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC16390.11816172367603357No Hit
TCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGC16140.11635937889757059No Hit
GTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATGAC16010.11542215961276982No Hit
CTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTGAC15760.11361981483430685No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC15620.11261050175836756No Hit
GGAGAAGGCAAAGCAGAATTAGCAGAAAAATTACACTGTTGGTTCGGTGG15480.1116011886824283No Hit
GTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATG15290.11023140665079642No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT15280.1101593128596579No Hit
GGGGAAAGGAGAAGACGTCCAAAAACTGGCAGAAGAACTGCAAAGCAACA15230.1097988439039653No Hit
GTCTTCTCCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTG15110.10893371841030307No Hit
GCTCTGCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGC15090.10878953082802602No Hit
CTGTTGTTCCCATTCCCGATAAGGGCTCTGTGATGAATCTTCTTTTTCTT15040.10842906187233343No Hit
TCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT15040.10842906187233343No Hit
AATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAA14830.10691509225842452No Hit
CTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAG14780.10655462330273191No Hit
GGCCATGAAAGCTCAGCGCTACTATATTGTCTCATGGTCATGTACCTGAA14500.10453599715085338No Hit
AATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATT14320.10323830891036001No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT14310.1031662151192215No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC14250.10273365237239039No Hit
CTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGG14250.10273365237239039No Hit
GAATTTGACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTT14010.1010034013850659No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT13910.10028246347368072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGG100.007075358145.1082145
ACACGTG306.548362E-10144.877987
GTACACG100.007111327144.86236
CGTTACC351.893568E-9124.378456145
GAGCAGA1600.0113.149191
TGACGCC458.367351E-11112.861946145
ACGTGCA203.8793465E-4108.658489
CACGTGC404.8457878E-9108.658488
AACACGT404.8494257E-9108.646736
GCGAGAA650.0100.45953145
AGCACGC5050.098.943931
GCACGCA5150.097.026192
CACGCAC5150.095.620013
AAACACG1000.094.15375
GGCCCCC259.3597395E-487.06492145
GCCGACC259.3597395E-487.06492145
GCAGCCC259.3597395E-487.06492145
CGATAAA608.258212E-1084.64645145
TAGAAAC4450.082.996023
GAAACAC1150.081.863944
Produced by FastQC (version 0.11.5)