FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9932_2_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9932_2_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392831
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA111070.7974406083724443No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT95450.6852949137404323No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT90690.651119913327604No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA82450.5919598285793467No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT80270.5763082527600262No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC68340.49065536306989144No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG64620.46394716946995No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC61900.4444185978054767No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT61880.4442750053667674No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA60890.43716717965065394No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG60330.4331465913667918No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA55860.40105368131524927No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT54700.3927253198701063No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT50220.3605606135992091No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA44950.3227240059992921No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT44830.3218624513670359No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA43530.31252894285092736No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC43430.31181098065738055No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT42710.3066416528638435No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG41890.3007543628767596No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC41620.2988158649541833No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG40700.29221061277355254No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA39610.2843848248638923No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA39380.28273351181873463No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT38380.2755538898832665No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT37190.2670101397800595No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG34330.24647642104462064No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC33040.23721470874786674No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA33020.2370711163091574No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT32710.23484543350916226No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG32500.233337712902714No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA31910.22910173596078776No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG31750.22795299645111286No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31530.2263734796253099No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG31360.22515294389628032No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT31120.22342983463176794No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA29190.2095731642963145No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT28830.20698850039954597No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG28330.20339868943181189No Hit
GTTGTTCCCATTCCCGATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTC28290.2031115045543932No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27300.19600367883827974No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC26530.1904753699479693No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA25370.18214700850282625No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT25170.18071108411573264No Hit
CTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGA24620.17676229205122518No Hit
CTCCTGGCACTGAGGATCTCGCTGCTCTGCTATGAGCTCTGTGTGAATGT24610.1766904958318705No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA24520.17604432985767834No Hit
GTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTG24430.17539816388348622No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT24080.17288529620607238No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC24070.1728134999867177No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA23990.17223913023188026No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG23650.1697980587738211No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC23640.1697262625544664No Hit
CCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA23640.1697262625544664No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT23550.16908009658027429No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC22980.16498771207705745No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT22760.16340819525125447No Hit
AGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC22680.162833825496417No Hit
CCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT22540.16182867842545146No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC22540.16182867842545146No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT22380.1606799389157766No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT22210.159459403186747No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAA21580.15493624136740208No Hit
GCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTC21510.1544336678319193No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA21480.15421827917385525No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG21410.1537157056383725No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA21370.15342852076095376No Hit
GTTTGGAGACACAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAG20870.14983870979321973No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA20870.14983870979321973No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT20570.14768482321257928No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA19420.13942825798679093No Hit
CTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAG19340.1388538882319535No Hit
CCCAAAGACCAGGAAAGAAAAAGAAGATTCATCACAGAGCCCTTATCGGG19210.13792053738034263No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA19020.1365564092126037No Hit
GTTTTTGCTGTGTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCC18970.13619742811583027No Hit
CTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATAG18890.13562305836099284No Hit
GCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGGATCTCGC18580.1333973755609977No Hit
TCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCATCTGC18440.13239222849003218No Hit
GTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGATA18010.12930499105778087No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT18010.12930499105778087No Hit
CATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGG17950.12887421374165278No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC17570.1261459574061749No Hit
GAAGATGGAGAAGGCAAAGCAGAATTAGCAGAAAAATTACACTGTTGGTT17520.1257869763094015No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG16990.1219817766836034No Hit
ACTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACA16830.1208330371739285No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA16810.12068944473521913No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT16600.11918172412877083No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC16430.11796118839974125No Hit
TCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGC16160.11602269047716485No Hit
GTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATGAC16050.11523293206426337No Hit
CTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTGAC15780.11329443414168697No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC15660.11243287950943079No Hit
GGAGAAGGCAAAGCAGAATTAGCAGAAAAATTACACTGTTGGTTCGGTGG15530.11149952865781995No Hit
GTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATG15310.10992001183201694No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT15290.1097764193933076No Hit
GGGGAAAGGAGAAGACGTCCAAAAACTGGCAGAAGAACTGCAAAGCAACA15260.10956103073524355No Hit
GCTCTGCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGC15190.10905845719976079No Hit
GTCTTCTCCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTG15140.10869947610298736No Hit
CTGTTGTTCCCATTCCCGATAAGGGCTCTGTGATGAATCTTCTTTTTCTT15120.10855588366427801No Hit
TCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT15090.10834049500621397No Hit
AATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAA14840.10654558952234693No Hit
CTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAG14820.10640199708363757No Hit
GGCCATGAAAGCTCAGCGCTACTATATTGTCTCATGGTCATGTACCTGAA14540.10439170294170651No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT14360.10309937099332223No Hit
AATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATT14350.10302757477396755No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC14310.10274038989654882No Hit
CTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGG14290.10259679745783945No Hit
GAATTTGACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTT14090.10116087307074585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCC100.0070771696145.09581145
ATAAGCC100.0070771696145.09581145
CGCACCC100.0070771696145.09581145
CGTTACC151.2268759E-4145.0958145
CGATAAA850.0119.490654145
AGCACGC5150.0113.9528961
ACACGTG458.54925E-11112.706447
AACACGT458.54925E-11112.706446
CACGCAC5300.0110.727823
GCACGCA5300.0110.727822
GGAGTTT203.856233E-4108.82185145
ACGAGCC501.9645086E-10101.439439
GCGAGAA554.1472958E-1092.33369145
CACGTGC554.1836756E-1092.2143558
GGCACCC406.094724E-790.684875145
ACACGAG800.090.567677
GAAACAC1300.089.171124
CAACGCA4250.088.652959
ACAACGC4300.087.618968
CGTGCTC259.3629275E-487.05747145
Produced by FastQC (version 0.11.5)