FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9932_1_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9932_1_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693059
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA87100.5144534242456996No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT75510.44599745194940044No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT68450.40429778288884205No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA62800.37092623470298436No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT58580.3460009367659367No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC56330.3327113821786482No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA53730.31735456354444824No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT51260.30276558584195823No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG47660.28150229850229674No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT45340.26779929110562595No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC44890.26514138018816824No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG42430.2506114671727329No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA42200.24925297937047675No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA40060.23661313634078907No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT39110.23100199107060063No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT38630.2281668860919791No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG36240.21405042588592602No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT35570.2100930918532668No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG35360.20885273342511984No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT34770.20536791688889755No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA34680.20483633470540602No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC34440.20341878221609525No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC33120.195622243524886No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA32950.19461814384495757No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT32940.19455907915790294No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG31870.1882391576430591No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT29700.17542212055220757No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA29380.17353205056645987No Hit
CCCAAGCAGCCACTCTACACTTGTTAGGGATTTTAGGCTCTTCTTGGCAC29370.17347298587940527No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA27620.16313666564484758No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG26810.15835242599342372No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT26410.15598983851123913No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT25390.14996524043166837No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC25040.14789797638475682No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG24580.1451810007802445No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA24570.1451219360931899No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA24550.14500380671908067No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT24380.1439997070391522No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG23790.1405148905029299No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22740.1343130983621953No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG22630.13366338680459453No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA22360.1320686402541199No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC22180.13100547588713682No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC22100.13053295839069992No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA21630.12775691809913298No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT21440.1266346890450953No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC21250.12551245999105762No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC20710.12232296689010838No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC20430.12066915565257916No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA20360.12025570284319682No Hit
CTCCTGGCACTGAGGATCTCGCTGCTCTGCTATGAGCTCTGTGTGAATGT20300.11990131472086915No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT20270.1197241206597053No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA19780.11682995099402914No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT19780.11682995099402914No Hit
GCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTC19760.11671182161991991No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG19680.116239304123483No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT19640.11600304537526453No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC19640.11600304537526453No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA19530.11535333381766377No Hit
CCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA19480.11505801038239069No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC19410.11464455757300838No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA19300.1139948460154076No Hit
GTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTG19260.11375858726718915No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC19030.11240009946493298No Hit
CTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGA18980.11210477602965992No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA18800.11104161166267684No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA18750.11074628822740377No Hit
AGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC18720.11056909416623992No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT18720.11056909416623992No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC18430.10885621824165607No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT18170.10732053637823608No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG18030.10649363075947148No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG17730.104721690147833No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT17650.10424917265139608No Hit
CCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT17500.10336320234557686No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT17490.10330413765852224No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT17480.10324507297146761No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA17080.10088248548928301No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC16940.1000555798705184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACC450.0145.11226145
GGACGGG100.007074904145.11226145
AGCACGC3250.0104.73211
AGCCACC352.7498754E-7103.65161145
GCACGCA3400.0100.1115652
GGCACCC451.0863005E-896.7415145
TGACGCC900.096.7415145
AACACGT1250.092.720966
CGATAAA651.6370905E-1189.29985145
GAAACAC2900.087.415144
AGAAGCC502.2575477E-887.06736145
CACGCAC4050.084.049233
GCAGCCC702.910383E-1182.92129145
ACACGTG1400.082.789027
AAACACG3150.082.781685
GAGCAGA1750.082.767011
AACGGCC451.2275941E-680.61792145
GCTGCCC1100.079.15214145
CACGTGC1500.077.274318
ACAACGC5600.076.325648
Produced by FastQC (version 0.11.5)