FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9906_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9906_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727191
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT68020.39381863383956955No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA44300.2564858200395903No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT38720.22417902826033717No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT38450.22261579640005075No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG34810.2015411150243372No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34600.2003252680218922No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT31530.1825507427956723No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC27490.15916016236768254No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT27260.15782852041262374No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGTGATTCTTCTACCTCCAAA26560.1537756970711404No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG26010.15059133587426057No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA25890.14989656615857772No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC25880.1498386686822708No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA25340.14671220496169793No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT25010.14480158824357003No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT24170.13993820023379No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG23500.13605906932122735No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC23120.13385896522156496No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA22710.13148516869298185No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC21980.12725865292257776No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA20430.11828454409500745No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA20410.11816874914239363No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT19800.11463700308767243No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA18700.10826828069391284No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA18680.10815248574129901No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA18590.10763140845453688No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT18270.10577868921271591No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT18240.1056049967837952No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT18220.10548920183118138No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT18010.10427335482873637No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC17750.10276802044475683No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA17640.10213114820538088No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA17610.10195745577646015No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC17480.10120478858447039No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC17320.1002784289635599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACCC600.0133.00027145
AGCACGC8900.0125.3393861
GCACGCA9050.0122.461532
CACGCAC9050.0121.661133
GAAACAC4250.0107.376314
CCTCACC501.9463187E-10101.56385145
AAACACG4700.097.0984045
TGACGCC301.5948017E-596.72748145
CGTTACC301.5948017E-596.72748145
CAGCGCC451.0873919E-896.72747145
GCCTCCC451.0873919E-896.72747145
ATAAGCC900.096.72747145
ACGCACT11950.093.3494
CGCACTT12150.091.218855
AACACGT1600.090.5559846
GGTCACC259.3644875E-487.054726145
GCTACCC259.3644875E-487.054726145
ACGTGCA1750.082.7844542
GAGCAGA1050.082.7844541
ACACGTG1800.080.494217
Produced by FastQC (version 0.11.5)