FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9861_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9861_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755308
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT75420.4296681835894327No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA39060.22252504973486134No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT38900.2216135287938071No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT36410.20742798414865085No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT32490.18509572109282246No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA29490.16800470344805585No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC27560.1570094820965893No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT26760.1524518773913182No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA26550.15125550615618455No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT26290.14977428462697145No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCTCCATTCTTCTGA25990.1480651828624948No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA25400.14470394939235734No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA25200.14356454821603956No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC23890.13610147051115815No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA23610.13450630886431325No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA23120.13171477598233472No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23020.13114507539417586No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT22680.12920809339443562No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT22160.1262456503360094No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT21650.12334017733639907No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT21610.12311229710113551No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC21250.12106137498376353No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC21130.12037773427797287No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG20610.11741529121954666No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT20450.11650377027849243No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC20380.11610497986678121No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC20300.1156492193962541No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG19920.11348435716125034No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG19910.11342738710243445No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC19900.11337041704361855No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT19470.11092070451453534No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA18960.10801523151492502No Hit
GATCTATACTAAATACTGATCAGAGAAACATGATTCTTGAAGAACAATGC18160.10345762680965392No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA18130.10328671663320625No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA18050.10283095616267914No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA18020.10266004598623148No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAACTACAGAAAACC17960.10231822563333615No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT17950.10226125557452025No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA17870.10180549510399314No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA17640.1004951837512277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTTCG100.0071116644144.861089
GGCACCC500.0130.59076145
TGACGCC650.0122.77763145
GCACGCA4450.0112.2920462
AGCACGC4450.0112.2920461
CACGCAC4550.0109.830343
CTAGGCC203.8558684E-4108.82563145
GAAACAC4300.0101.0572364
AAACACG4450.099.281165
AACACGT1500.096.5713046
CCTCACC554.1472958E-1092.3369145
ATAAGCC554.1472958E-1092.3369145
GCGAGAA259.362042E-487.06051145
GGACCCC259.362042E-487.06051145
ACACGTG1700.085.21247
AACACGA3500.084.844796
TGTGCCC608.258212E-1084.64216145
CGCACCC353.4261655E-582.914764145
GAGCAGA1500.082.0762561
GCAGCCC451.2281871E-680.61159145
Produced by FastQC (version 0.11.5)