FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9841_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9841_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580475
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA121410.768186779291036No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT80140.5070627501225897No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG68560.43379363798857934No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT47540.3007956468783119No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT42740.27042503044970656No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC34660.2193011594615543No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA30710.1943086730255145No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC30150.1907654344421772No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA29840.18880399879782978No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA29520.18677929103592275No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT28640.18121134469067843No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT28560.18070516775020168No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA28200.1784273715180563No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA27570.17444122811180182No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC27310.1727961530552524No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT26430.16722820671000807No Hit
GTGCTATACTACAAAAATCCCGGAACCAAATTGGGCTGTCTCTGGTTATT25880.16374824024423038No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC25570.16178680459988296No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC25330.16026827377845268No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA25270.1598886410730951No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA25080.15868647083946283No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC25040.15843338236922444No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG24360.154130878375172No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT23760.15033455132159634No Hit
GAGTAAACATGAAAATAAGAATAAAGGGGCCAAATAAAGAGACAATAAAC23750.15027127920403677No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC23720.15008146285135798No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA23620.14944874167576203No Hit
GTATTAGTAGTTGAAAACTTGGCTAAGAACATCTGTGAAAATCTAGAACA23530.1488792926177257No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT22840.14451351650611366No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC22740.14388079533051773No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA22360.14147645486325314No Hit
ATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT22320.14122336639301475No Hit
ATTTAGTATTGTCTCCTGTTACTGTCATGCTGATCCCTCCTGGTGGGCAG22180.14033755674718046No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT22010.13926193074866733No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC22000.13919865863110775No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT21930.13875575380819058No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC21800.13793321627991587No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC21490.13597178063556842No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG21350.1350859709897341No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTACATA20680.13084673911324127No Hit
GTCTCTGGTTATTCTTTCAGTCATAGCCAAAAAGATTCGTGGATTTAAGC20630.1305303785254433No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC20460.12945475252693017No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG20270.1282525822932979No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG20210.12787294958794035No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG20210.12787294958794035No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC20030.12673405147186764No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT19930.12610133029627169No Hit
AATAAAGAGACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCA19910.1259747860611525No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC19700.12464607159240101No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT19370.1225580917129344No Hit
CAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGAAAATAAGA19200.1214824657144213No Hit
GTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAA19150.12116610512662332No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG19060.12059665606858698No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC18400.11642069630965375No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT18370.11623087995697495No Hit
GTTAGATACAAATCCATCTCTATAGAACATGCTTGTAAATTCAAACATTC18220.11528179819358104No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA18040.11414290007750834No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG18000.11388981160726996No Hit
GGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGA17960.11363672313703158No Hit
CATCAAAAGAGCATTGTCATTAAACACAATGACAAAAGATGCTGAAAGGG17710.11205492019804172No Hit
GTTCATAGCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCA17450.11040984514149228No Hit
GGACAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGAAAATA17280.10933421914297917No Hit
GAATAGAGGAGACCCTTTCCTTACTTCGATGAAATTTTCTCCTCCTCCCT16870.1067400623230358No Hit
ATATTAAACATTCCCATCATCATCCCAGGCGACAAAGATGCTGTTCCTTC16840.10655024597035702No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT16790.10623388538255904No Hit
TAATAATACTGCTGCTCTTTTCAACCACCATGCGTTCTGGTGGTAGGCTG16750.10598079691232067No Hit
GTATGGAAGGGATAAATGATTTTTACCGAACATGTAAATTATTGGGAATA16700.10566443632452269No Hit
TCTTTTTAGTTTGCCCCTTTCAGCATCTTTTGTCATTGTGTTTAATGACA16680.1055378920894035No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT16610.10509498726648633No Hit
TGTTTATTCCCAATAATTTACATGTTCGGTAAAAATCATTTATCCCTTCC16480.1042724497382116No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG16400.10376627279773484No Hit
CTTATGGGATGGACTGCAATCTTCTGATGATTTTGCTTTGTTTGTTAATG16380.10363972856261568No Hit
ACCAGAGACAGCCCAATTTGGTTCCGGGATTTTTGTAGTATAGCACCTGT16070.10167829291826823No Hit
CTCCATTTCATGGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAAG15890.10053939480219555No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTT15870.10041285056707634No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG15870.10041285056707634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTG100.0070796628145.07932145
GCGGTTT151.227462E-4145.07932145
ATGACCG100.0070796628145.07932145
TGACGCC850.0110.943145
GCCGACC404.798494E-9108.80949145
CTAGGCC203.8580733E-4108.80949145
ACGTGCC800.0108.692439
AAACACG5650.0100.019685
ACACGAG4000.099.625277
ACGAGCC2150.097.738939
CGACGAA900.096.71955145
AACGGCC607.2759576E-1296.71955145
GTAGCCC451.0879376E-896.71955145
GAGCAGA1500.091.7672961
CACGAGC4450.089.556478
AAGTACC750.087.04759145
ACGAGCG1500.086.953949
GAAACAC6450.086.488154
AAACCCC1950.085.5596145
ACAACGC9250.083.817818
Produced by FastQC (version 0.11.5)