FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9841_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9841_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586487
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA121620.766599411151809No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT80370.5065909774237041No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG68840.4339146806749756No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT47760.30104249199646765No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT42780.2696523829063837No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC34790.21928953719759442No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA30750.19382446877913276No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC30200.19035768966275804No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA29890.1884036868880741No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA29620.18670181350367193No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT28770.18134406396018374No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT28670.18071374048447925No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA28280.1782554789292317No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA27660.1743474733798638No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC27390.17264559999546167No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT26510.16709875340926209No Hit
GTGCTATACTACAAAAATCCCGGAACCAAATTGGGCTGTCTCTGGTTATT25930.163442877250176No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC25660.1617410038657739No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC25440.16035429221922398No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA25360.1598500334386604No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA25150.15852635413968094No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC25120.1583372570969696No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG24410.15386196041946767No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT23850.1503321489555225No Hit
GAGTAAACATGAAAATAAGAATAAAGGGGCCAAATAAAGAGACAATAAAC23830.1502060842603816No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC23730.14957576078467708No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA23670.14919756669925438No Hit
GTATTAGTAGTTGAAAACTTGGCTAAGAACATCTGTGAAAATCTAGAACA23610.14881937261383169No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT22900.14434407593632975No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC22800.1437137524606253No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA22420.14131852325294816No Hit
ATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT22380.14106639386266637No Hit
ATTTAGTATTGTCTCCTGTTACTGTCATGCTGATCCCTCCTGGTGGGCAG22260.140310005691821No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT22080.1391754234355529No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC22040.1389232940452711No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT21970.13848206761227794No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC21850.13772567944143255No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC21570.13596077370945997No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG21410.13495225614833276No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTACATA20740.13072908886111262No Hit
GTCTCTGGTTATTCTTTCAGTCATAGCCAAAAAGATTCGTGGATTTAAGC20700.13047695947083082No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC20510.12927934486699227No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG20330.1281447626107242No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG20250.1276405038301606No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG20240.12757747148259016No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC20080.12656895392146295No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT19980.12593863044575845No Hit
AATAAAGAGACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCA19960.12581256575061756No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC19760.12455191879920857No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT19420.12240881898181327No Hit
CAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGAAAATAAGA19250.12133726907311564No Hit
GTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAA19210.12108513968283384No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG19120.1205178485546998No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC18470.11642074596262056No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT18440.11623164891990921No Hit
GTTAGATACAAATCCATCTCTATAGAACATGCTTGTAAATTCAAACATTC18270.11516009901121156No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA18120.11421461379765482No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG18060.11383641971223213No Hit
GGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGA17990.11339519327923898No Hit
CATCAAAAGAGCATTGTCATTAAACACAATGACAAAAGATGCTGAAAGGG17760.11194544928511863No Hit
GTTCATAGCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCA17490.1102435759007165No Hit
GGACAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGAAAATA17320.10917202599201885No Hit
ATATTAAACATTCCCATCATCATCCCAGGCGACAAAGATGCTGTTCCTTC16930.10671376443677132No Hit
GAATAGAGGAGACCCTTTCCTTACTTCGATGAAATTTTCTCCTCCTCCCT16920.10665073208920087No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT16810.10595737626592591No Hit
TAATAATACTGCTGCTCTTTTCAACCACCATGCGTTCTGGTGGTAGGCTG16790.10583131157078501No Hit
TCTTTTTAGTTTGCCCCTTTCAGCATCTTTTGTCATTGTGTTTAATGACA16730.10545311748536232No Hit
GTATGGAAGGGATAAATGATTTTTACCGAACATGTAAATTATTGGGAATA16720.10539008513779187No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT16690.10520098809508052No Hit
TGTTTATTCCCAATAATTTACATGTTCGGTAAAAATCATTTATCCCTTCC16520.10412943818638287No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG16440.10362517940581926No Hit
CTTATGGGATGGACTGCAATCTTCTGATGATTTTGCTTTGTTTGTTAATG16420.10349911471067837No Hit
ACCAGAGACAGCCCAATTTGGTTCCGGGATTTTTGTAGTATAGCACCTGT16090.10141904724085353No Hit
CTCCATTTCATGGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAAG15950.10053659437486723No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTT15910.10028446498458543No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG15910.10028446498458543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC100.00708058145.07306145
GTGCCCC100.00708058145.07306145
CGTATGA100.0071080057144.885591
GTGACCC450.0128.95383145
GCCGACC600.0120.89421145
GACGCTT203.8587395E-4108.804794145
AGTGTAA203.8587395E-4108.804794145
TGACGCC850.093.870804145
AACGGCC554.1472958E-1092.31922145
AAACCCC1750.091.188774145
GAGCAGA1150.088.191231
ACACGAG3350.086.5097667
ACAACGC9650.084.842768
CCTCACC608.258212E-1084.625946145
AAACACG6700.083.2577745
ACGTGCC1400.082.807439
CAACGCA9900.082.702879
AACACGT3400.080.973146
CACGAGC3600.080.504688
ACGAGCC1550.079.468429
Produced by FastQC (version 0.11.5)