FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9839_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9839_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719019
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT93690.5450201539366347No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT78770.4582264652106812No Hit
AGCACGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT58710.34153200168235487No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT48480.28202131564572586No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC46860.2725973360387523No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT46470.27032860020744387No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAAGC44330.25787963949205917No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC35730.20785110577602692No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGAT34980.2034881522542799No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA33240.19336610008382688No Hit
GATATACAAAAAGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCCAA33180.19301706380208714No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT32200.18731613786700438No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT30930.17992820323684613No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT28080.16334897985420754No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATCCGTATTTTCATGTTTATTC27500.15997496246405654No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC27150.15793891748724126No Hit
TTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCATTGTTTCTTTGGC27040.15729901763738505No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA26970.15689180864202198No Hit
GTAAGAAATACAGTAAAATTAGATTTAATGCAATTCTTCTATCTCCAAAA26390.15351779125187098No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA26300.15299423682926136No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA25800.1500856011480967No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT25730.14967839215273362No Hit
CTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATCTTA25490.14828224702577458No Hit
AAGTTACACTGTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCAGCCTT25360.14752600174867178No Hit
ATCTTATGCATGGTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAG25180.1464788929034525No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCAT24510.14258132109069183No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTAACACATCTTC24450.1422322848089521No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA23700.13786933128720508No Hit
CTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTTGCTTTCATTGAC22950.1335063777654581No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT22360.1300741876616838No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG22050.1282708335393617No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA21520.12518767971732714No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGATGCAAGTAAAA21100.12274442574514884No Hit
CATAAAGCACAGAGCGTTCCTAGTTTTACTTGCATCGAATAATTTCCAGG21040.12239538946340908No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG20960.12193000775442273No Hit
CTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCATC20860.12134828061818981No Hit
AGCAGGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT20800.12099924433645003No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG20740.12065020805471027No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC20220.11762522694629901No Hit
GTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAA20060.11669446352832634No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGATGC19870.11558918196948377No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA19780.11506562754687412No Hit
CTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTCTGTCTC19710.11465841855151106No Hit
ATACTAAACAGATCAGGACAAGGTATCTGAGCCTTTAGTCTTTTTGTTTT19540.11366948241991508No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA18870.10977191060715444No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG18820.10948104703903795No Hit
GTTGTTCCCATTCCTGATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTC18710.10884114718918174No Hit
ATGCTTAACTGATATACAAAAAGCACTAATTGGTGCCTCTATATGCTTTT18530.10779403834396245No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA18020.10482722994917451No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17780.10343108482221547No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG17460.10156955798627007No Hit
GATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTCCTGGTCTTTGGGTTT17290.10058062185467409No Hit
CCATGAGACAGTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA17290.10058062185467409No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA17210.10011524014568775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCC306.493792E-10145.0705145
CGTTGGG100.0070810043145.0705145
AGTGGGG151.2277781E-4145.0705145
GCCACCC100.0070810043145.0705145
AGCACGC7150.0114.516081
CACGCAC7200.0113.720833
GCACGCA7200.0113.720832
AACGGCC203.8590655E-4108.80287145
GTAACAA8950.0106.057734
GAGCAGA1450.099.943851
ACGCACT8300.099.52244
CGCACTT8550.096.6152045
GCCGACC406.1016726E-790.66906145
TCTGACC651.6370905E-1189.274155145
GGCACCC259.369794E-487.0423145
AGACGGG259.369794E-487.0423145
CCTGCCC259.369794E-487.0423145
TACACCC259.369794E-487.0423145
ACACGAG3400.083.122327
AAACACG4450.083.045645
Produced by FastQC (version 0.11.5)