FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9839_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9839_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724288
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT93760.54376067107119No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT78930.4577541570781679No Hit
AGCACGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT58780.34089432855764235No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT48550.2815654925395294No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC46950.27228630020043054No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT46540.2699085071635365No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAAGC44430.2576715722663499No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC35830.20779591344369386No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGAT35040.20321431222626382No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA33320.1932391804617326No Hit
GATATACAAAAAGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCCAA33230.1927172258926583No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT32240.1869757256328409No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT31020.17990034147427808No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT28110.16302381040754213No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATCCGTATTTTCATGTTTATTC27590.160008072897335No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC27210.15780426471679904No Hit
TTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCATTGTTTCTTTGGC27090.15710832529136665No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA27010.1566443656744117No Hit
GTAAGAAATACAGTAAAATTAGATTTAATGCAATTCTTCTATCTCCAAAA26470.15351263825996586No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA26320.15264271397817533No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA25850.14991695122856505No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT25770.14945299161161013No Hit
CTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATCTTA25510.14794512285650657No Hit
AAGTTACACTGTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCAGCCTT25400.14730717838319352No Hit
ATCTTATGCATGGTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAG25230.14632126419716426No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCAT24540.14231961250092792No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTAACACATCTTC24500.14208763269245045No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA23750.13773801128349789No Hit
CTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTTGCTTTCATTGAC22990.13333039492242596No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT22380.12979270284314454No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG22090.12811084923168287No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA21560.12503711676935642No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGATGCAAGTAAAA21140.12260132878034295No Hit
CATAAAGCACAGAGCGTTCCTAGTTTTACTTGCATCGAATAATTTCCAGG21110.12242734392398485No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG20980.12167340954643308No Hit
CTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCATC20880.12109346002523941No Hit
AGCAGGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT20840.12086148021676195No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG20780.12051351050404573No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC20270.11755576794595798No Hit
GTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAA20090.11651185880780937No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGATGC19950.11569992947813822No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA19820.11494599510058644No Hit
CTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTCTGTCTC19730.11442404053151214No Hit
ATACTAAACAGATCAGGACAAGGTATCTGAGCCTTTAGTCTTTTTGTTTT19570.11349612129760225No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA18900.10961045950560463No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG18880.10949446960136591No Hit
GTTGTTCCCATTCCTGATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTC18770.10885652512805286No Hit
ATGCTTAACTGATATACAAAAAGCACTAATTGGTGCCTCTATATGCTTTT18600.10787061094202362No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA18060.10473888352757776No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17810.10328900972459358No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG17460.1012591864004157No Hit
CCATGAGACAGTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA17330.10050525202286392No Hit
GATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTCCTGGTCTTTGGGTTT17320.10044725707074456No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA17250.10004129240590899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCC100.007073006145.12537145
AGCACGC7100.0110.1561661
GCACGCA7200.0108.626222
CACGCAC7250.0107.8770753
GTAACAA8950.0101.956794
CGCACTT7750.0100.928985
ACGCACT7900.0100.840324
GAAACAC4400.0100.402784
AAACACG4550.097.098435
GATAGCC301.5929385E-596.750244145
TAGAAAC5000.091.2460252
CTGGTCC406.0879574E-790.70335145
TGACGCC651.4551915E-1189.30791145
CAGCGCC259.3557325E-487.07522145
GCTAACC259.3557325E-487.07522145
GTGACCC259.3557325E-487.07522145
ATCGAAA259.3557325E-487.07522145
GACAAAC259.430474E-486.9009861
AACACGA3800.085.769986
ACACGAG3850.084.6617
Produced by FastQC (version 0.11.5)