FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9825_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9825_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402714
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT108680.45232183272749066No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA81870.34073968021162737No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT80240.33395568511275164No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA70830.29479163978734046No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT65860.2741066976760447No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT65790.27381536046320953No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA61660.25662646490593555No Hit
TTCTAATACTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACC60120.25021704622356217No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA58890.24509783519803024No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT57780.2404780593945014No Hit
ATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTT57700.24014510257983263No Hit
GTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACATT55870.2325287154442851No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA52630.21904396445020088No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC50760.21126109890731898No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC50650.21080328328714945No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG48410.20148049247642458No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC47810.198983316366409No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT43510.1810868875779639No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC43110.1794221035046202No Hit
AGTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATT42710.17775731943127646No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT42220.1757179589414304No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG39210.16319045878951885No Hit
GTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTC37820.1574053341346494No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA37800.1573220949309822No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT37380.1555740716539713No Hit
ATACATAAGATTGGAATATGGGACGGAGAAGAGGAGTTCCATGTAAGATG37010.15403414638612836No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC36570.15220288390545025No Hit
ATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA35780.14891493536059638No Hit
GTACTAAATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTG35250.14670909646341596No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA35130.14620966124141282No Hit
CTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACA34960.14550212801024176No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG34810.14487783398273785No Hit
ATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAA34350.14296333229839256No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA32680.1360128587921825No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT32460.13509722755184347No Hit
CTTATGTATTGTTCCGTTCCCAATTAATATTTCTTCATCATTTTTGAATC32070.13347406308033333No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT31950.1329746278583302No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA31540.1312682241831529No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA30930.1287294284713037No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30720.12785541683279825No Hit
GTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTTCC30640.1275224600181295No Hit
GCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCACAATATGAAGT29850.12423451147327565No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA29780.12394317426044049No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG29110.12115466093758974No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT28360.11803319080007026No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT28260.11761699478173432No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC28010.1165765047358945No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT27630.11499495986621794No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG27610.11491172066255076No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG27210.11324693658920704No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC27070.11266426216353673No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA27040.11253940335803594No Hit
GTACTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCTCC26690.1110827172938602No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA26540.1104584232663563No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA26400.109875748840686No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG26330.10958441162785085No Hit
GAACAATACATAAGATTGGAATATGGGACGGAGAAGAGGAGTTCCATGTA25920.10787800795267351No Hit
CTACAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGAT25710.10700399631416806No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT25320.10538083184265792No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG25150.10467329861148685No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT24840.10338309095464546No Hit
CTATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTAC24810.10325823214914467No Hit
GTATTGTTCCGTTCCCAATTAATATTTCTTCATCATTTTTGAATCCTCTT24800.10321661254731108No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT24750.10300851453814311No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24720.10288365573264233No Hit
GACTACAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAG24690.10275879692714157No Hit
TCCTAGTGTCTTGGGAAAATACCATGCACAAAATTATTAAATCTTTCATG24620.10246745971430642No Hit
GTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAA24570.10225936170513844No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATAT24330.10126049126113221No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC24140.10046971882629392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAT100.0070787314145.08727145
CACGCAC10450.0122.69143
GCACGCA10450.0122.69142
AGCACGC10500.0122.104611
CAGCGCC501.8189894E-12116.06981145
CGCACTG501.8189894E-12115.898315
TGACGCC800.0108.81545145
CGTTACC404.798494E-9108.81545145
CTAGGCC352.7530587E-7103.63376145
GAAACAC7250.0102.9097064
AAACACG7450.0100.147035
GCTGCCC1100.098.92313145
ACACGTG2350.095.5604257
AACACGT2350.095.558436
GGCACCC554.1472958E-1092.328255145
AACACGA5900.088.4006966
GCGAGAA259.36595E-487.05235145
TCGAGCC259.36595E-487.05235145
CACAGCG259.421127E-486.923733
ACACGAG6550.079.629777
Produced by FastQC (version 0.11.5)