FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9825_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9825_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410537
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT108930.45189101017740035No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA82040.3403391028638017No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT80360.3333697014399696No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA70990.29449869468919165No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT66000.2737979130791189No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT65980.2737149440145495No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA61790.2563329249872539No Hit
TTCTAATACTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACC60230.2498613379508383No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA59030.24488319407667253No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT57960.24044434912220805No Hit
ATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTT57840.23994653473479147No Hit
GTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACATT55970.23218892719754974No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA52730.2187479387373021No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC50870.2110318157323451No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC50790.21069993947406737No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG48540.2013659197100065No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC47900.19871090964378477No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT43580.1807895916967879No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC43260.17946208666367702No Hit
AGTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATT42770.177429344581726No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT42340.17564550969348325No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG39290.16299272734664516No Hit
GTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTC37900.15722637735906977No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA37880.15714340829450035No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT37470.15544254247082703No Hit
ATACATAAGATTGGAATATGGGACGGAGAAGAGGAGTTCCATGTAAGATG37080.15382464571172316No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC36590.15179190362977213No Hit
ATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA35880.14884650183755738No Hit
GTACTAAATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTG35310.1464818834973286No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA35260.146274460835905No Hit
CTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACA35040.1453618011256413No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG34860.14461507954451644No Hit
ATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAA34370.1425823374625654No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA32800.13606926589386514No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT32540.13499066805446255No Hit
CTTATGTATTGTTCCGTTCCCAATTAATATTTCTTCATCATTTTTGAATC32140.13333128676307396No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT32060.13299941050479624No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA31600.13109112201969933No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA30990.12856056555033174No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30770.127647905840068No Hit
GTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTTCC30710.1273989986463597No Hit
GCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCACAATATGAAGT29920.12412172059586722No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA29840.12378984433758951No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG29180.12105186520679832No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT28430.11794052528544469No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT28320.11748419543031283No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC28060.11640559759091024No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG27690.1148706698963758No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT27680.11482918536409106No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG27270.11312831954041774No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC27110.11246456702386232No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA27080.11234011342700818No Hit
GTACTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCTCC26730.11088815479704316No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA26600.11034885587734185No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA26430.10964361882850171No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG26390.10947768069936284No Hit
GAACAATACATAAGATTGGAATATGGGACGGAGAAGAGGAGTTCCATGTA26000.10785978394025894No Hit
CTACAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGAT25770.10690563969771051No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT25380.10528774293860663No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG25230.1046654749543359No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT24910.10333796992122503No Hit
GTATTGTTCCGTTCCCAATTAATATTTCTTCATCATTTTTGAATCCTCTT24870.10317203179208616No Hit
CTATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTAC24860.10313054725980145No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24800.10288164006609316No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT24780.10279867100152372No Hit
GACTACAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAG24730.10259124834010014No Hit
TCCTAGTGTCTTGGGAAAATACCATGCACAAAATTATTAAATCTTTCATG24670.10234234114639186No Hit
GTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAA24610.10209343395268357No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATAT24380.10113928971013511No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC24200.10039256812901026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG151.2347453E-4144.866381
AGCACGC11500.0124.08121
GCACGCA11550.0122.292342
CACGCAC11800.0120.931613
GCCGACC458.367351E-11112.86113145
GTGACCC203.8553777E-4108.830376145
ATAAGCC750.0106.411934145
CCCGCCC301.5940597E-596.73811145
GAAACAC7000.096.240654
CACCCCC1900.095.46524145
AAACACG7150.094.227485
ACACGTG2100.089.695927
AACACGT2150.087.609986
CAGCGCC259.3608495E-487.0643145
GGGCCCC502.2584572E-887.0643145
GAGCAGA2200.082.310451
CTAGGCC451.2280088E-680.61509145
GGAGTTT554.3746695E-879.14937145
CAAGCCC554.3746695E-879.14937145
GGCACCC651.5570549E-978.13463145
Produced by FastQC (version 0.11.5)