FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9823_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9823_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567594
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT78450.5004484579553125No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT65880.4202618790324536No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT59290.37822293272365165No Hit
GTTCATAGCTGAGACCATTTGCATTTCTCGTCTCACTCCAGGCACTGAAG44600.2845124439108596No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT42860.2734126310766691No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA40370.2575284161587758No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAGGC39650.2529353901584211No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA39450.25165954960276704No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA38050.2427286657131885No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA37700.2404959447407939No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT37130.23685979915717975No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT36970.23583912671265647No Hit
CTCCAATGTTGCTTTGTAGCTCTTCTGCCAGCTTTTGGACGTCTTCTCCT33830.21580842998888744No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT33680.2148515495721469No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA33260.2121722844052733No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC30770.19628806948738No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC30360.19367259634828915No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC28560.18219003134740244No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC28050.17893663793048456No Hit
CCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGA27040.17249364312443147No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT26050.16617823237394377No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG25990.16579548020724755No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA25940.16547652006833402No Hit
TTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGATCTCAGCACT24680.15743872456771332No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGTAGCT24440.15590771590092845No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC24100.15373878695631651No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT22850.14576478348347852No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT22800.145445823344565No Hit
GATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCCAA22750.14512686320565146No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGTTTCTTTGGC22240.14187346978873355No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA22050.1406614212608622No Hit
AAATTACACTGTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCTGCCTT21290.1358132271493767No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT20480.13064607289897767No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGAAAG20230.12905127220441007No Hit
ATTCATCACAGAGCCCTTATCAGGAATGGGAACAACAGCAACAAAAAAGA20020.1277116396209733No Hit
GTGCTTTATGCGAGAAACAAGCATCACATTCACACAGGGCTCATAGCAGA19400.12375653389844565No Hit
CCATGAGACAGTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA19110.12190656509274723No Hit
GCCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAA19020.1213324368427029No Hit
CTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCATC18910.12063072453709314No Hit
GTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGATA18560.11839800356469853No Hit
GCCCTTATCAGGAATGGGAACAACAGCAACAAAAAAGAAAGGCCTGATTC18320.11686699489791362No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA18310.11680320287013092No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG18090.11539977825891143No Hit
CTTTATTTGATTCAAATGCCCTATTGTCCAAGCCATGAAATGGAGAGCTG18050.11514461014778062No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG17950.11450668986995358No Hit
CCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGTAGCTCTTCTGCCAGC17610.11233776092534165No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT17600.11227396889755892No Hit
ACATTGGAGTGCTGAGATCTCTTGGGGCAAGTCAAAAGAATGGGGAAGGG17540.11189121673086272No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17390.11093433631412215No Hit
CTGATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCC17200.10972228778625079No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG16480.1051292617858961No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT16420.10474650961919986No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA16300.10398100528580742No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG16280.10385342123024202No Hit
CTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATCTTA16190.10327929298019768No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCAT16060.10244999661902254No Hit
TAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAATGCAAA16030.10225862053567442No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAGTATAGTAGCGC15860.10117415606336845No Hit
CTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCA15850.10111036403558575No Hit
CTCTTGGGGCAAGTCAAAAGAATGGGGAAGGGATTGCAAAGGATGTAATG15810.10085519592445494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGG256.448692E-6116.06265145
TGACGCC501.9463187E-10101.554825145
ACACGAG1950.096.617057
AAACACG2900.094.948215
AACACGA2050.091.9040156
GGTACCC406.0994535E-790.67394145
GAAACAC3050.090.275744
CACGAGC2150.087.632228
GCTGCCC502.2610038E-887.04699145
GGTGCGT750.086.955346
CGGTGCG750.086.952575
CAACGCA2850.086.452359
GTCGCCC353.4286917E-582.90189145
AACGGCC353.4286917E-582.90189145
ACGAGCC1050.082.819899
ACACGTG1050.082.8146067
GAGCAGA1500.082.111391
GCCTCCC451.2292603E-680.59906145
GTAACAA3900.079.889764
AACACGT1100.079.050316
Produced by FastQC (version 0.11.5)