FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9823_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9823_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575072
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT78670.4994692306129498No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT66100.4196633550720221No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT59380.3769986387923854No Hit
GTTCATAGCTGAGACCATTTGCATTTCTCGTCTCACTCCAGGCACTGAAG44770.2842409743808537No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT42960.27274943621624914No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA40440.2567501676113854No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAGGC39800.25268686129903906No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA39490.25071869730399626No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA38200.24252859551817316No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA37800.23998902907295666No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT37300.23681457101643608No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT37110.23560827695495828No Hit
CTCCAATGTTGCTTTGTAGCTCTTCTGCCAGCTTTTGGACGTCTTCTCCT33940.21548221287661773No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT33750.21427591881513988No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA33300.21141890656427134No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC30860.19592755124845088No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC30420.19313402815871275No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC28650.18189644663862983No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC28080.17827756445419637No Hit
CCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGA27140.17230958330793766No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT26110.16577019971150525No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA26100.16570671055037484No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG26050.1653892647447228No Hit
TTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGATCTCAGCACT24700.1568182279921172No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGTAGCT24470.15535797728611772No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC24170.15345330245220537No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT22960.14577111395542552No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT22900.14539017898864307No Hit
GATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCCAA22780.14462830905507812No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGTTTCTTTGGC22320.1417078076430792No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA22130.14050151358160134No Hit
AAATTACACTGTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCTGCCTT21310.13529540236890758No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT20530.13034324780073547No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGAAAG20290.12881950793360558No Hit
ATTCATCACAGAGCCCTTATCAGGAATGGGAACAACAGCAACAAAAAAGA20050.1272957680664757No Hit
GTGCTTTATGCGAGAAACAAGCATCACATTCACACAGGGCTCATAGCAGA19420.12329595091525976No Hit
CCATGAGACAGTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA19190.12183570020926028No Hit
GCCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAA19090.12120080859795616No Hit
CTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCATC19010.12069289530891286No Hit
GTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGATA18580.11796286138030516No Hit
GCCCTTATCAGGAATGGGAACAACAGCAACAAAAAAGAAAGGCCTGATTC18380.11669307815769692No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA18330.11637563235204487No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG18190.1154867840962191No Hit
CTTTATTTGATTCAAATGCCCTATTGTCCAAGCCATGAAATGGAGAGCTG18110.11497887080717581No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG17990.11421700087361085No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT17660.11212185855630727No Hit
CCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGTAGCTCTTCTGCCAGC17640.11199488023404644No Hit
ACATTGGAGTGCTGAGATCTCTTGGGGCAAGTCAAAAGAATGGGGAAGGG17560.11148696694500315No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17460.11085207533369902No Hit
CTGATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCC17250.10951880294996039No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG16500.1047571158651795No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT16480.10463013754291868No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA16330.10367780012596249No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG16320.10361431096483208No Hit
CTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATCTTA16250.1031698868369192No Hit
TAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAATGCAAA16150.1025349952256151No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCAT16120.10234452774222384No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAGTATAGTAGCGC15900.1009477661973548No Hit
CTCTTGGGGCAAGTCAAAAGAATGGGGAAGGGATTGCAAAGGATGTAATG15880.10082078787509395No Hit
CTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCA15880.10082078787509395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC100.0070788027145.0852145
GGCGAGG100.0070788027145.0852145
GAGCAGA1600.0117.705841
GCACGCA1400.0113.825432
CACGCAC1450.0109.900423
AGCACGC1550.0102.810071
AACGGCC451.0875738E-896.723465145
ACACGAG2800.095.732037
ACACGTG1150.094.4946067
GAAACAC4100.093.637654
AACACGA2900.092.427996
AACACGT1200.090.554466
AAACACG4200.089.6891865
GTCGCCC259.365875E-487.051125145
TGACGCC502.2602762E-887.051125145
GCCTCCC502.2602762E-887.051125145
CACGTGC1250.086.940558
CACGAGC2950.085.958188
CAACGCA2050.084.828149
ACGTGCC950.083.8979959
Produced by FastQC (version 0.11.5)