FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9815_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9815_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055714
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC76560.37242534710567715No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG75970.3695552980618899No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG71300.3468381302068284No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA65200.3171647417880114No Hit
GCTATAACTATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCA61870.3009659904052801No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG52420.2549965608056373No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT44610.21700489464974212No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG41760.20314109842127845No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG40930.19910357180035745No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA40480.1969145513432316No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC39020.1898123960823344No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC33660.16373873019301322No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT32230.15678250962925777No Hit
ATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA31180.15167479522929747No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT30570.14870745638741575No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT29800.1449617991607782No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG29210.142091750116991No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG28660.13941628066939274No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT28470.13849202758749515No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT28320.13776235410178653No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG27040.13153580702373968No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA26600.12939543146566107No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC26570.12924949676851935No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG26070.1268172518161573No Hit
GTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATA26040.12667131711901558No Hit
GTATTGGCTTCTAGTGGCCTTGGGGACTAAAGATTGAAATGGTTCAAATT26010.12652538242187386No Hit
ATATACAGGAGAGTCGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA25670.12487145585426766No Hit
GCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC25610.12457958645998424No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA25480.12394720277237009No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG25110.12214734150762217No Hit
GTCTAAGAATGTCCACCATCCTTGTTCCGCCAATTTGCGTGCTGTGGCAC25000.12161224761810252No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA24410.11874219857431531No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC24330.11835303938193738No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT24270.11806116998765394No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC23490.11426686786196912No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTA23360.113634484174355No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA22640.11013205144295363No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC22420.10906186366391434No Hit
CAATAGGATGGGAGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG22330.10862405957248918No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTAAGGAAGGAGTTGAA21840.10624045951917437No Hit
TAATAATACATTCCCACGTTGGTCTCGAACTCTCAAAAACCGATCAATGC21480.10448924315347369No Hit
CCTATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCT21200.10312718598015093No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT20830.10132732471540301No Hit
GTACTGGAGTATTCATCCACACCCATTTTGCTGATTCTTATTCCTCTCAT20680.1005976512296944No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA20640.10040307163350544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCC306.493792E-10145.07524145
TCACGCC750.0125.73189145
GGTCTTT1400.0113.9877145
GCTGACC2750.0108.14701145
CGTCACC800.099.739235145
ACTTACC259.3688356E-487.04515145
GAGCACC502.2617314E-887.04515145
CGACGGG608.276402E-1084.62723145
AGCGAAA4800.082.986641
TTGGGCC353.4293043E-582.90015145
GAAGCCC801.8189894E-1281.60483145
ATCACCC801.8189894E-1281.60483145
AACCGCC451.2295513E-680.59736145
CGAAAGC5100.078.1145863
GCTGTCC1150.075.691444145
CCAAGGG406.647748E-572.53763145
TTATATT14250.071.6640852
TATATTC14400.070.421473
TGACGCC554.0506347E-665.9433145
GAGCGAA1550.065.415861
Produced by FastQC (version 0.11.5)