FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9810_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9810_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157948
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC81440.37739556282171766No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC72640.33661608157379136No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA72170.3344380865525953No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT71160.3297577142730038No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC66700.30908993173144117No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC61940.287031939601881No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT61920.28693925896268124No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA60640.281007698053892No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA57910.2683567908031148No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA57260.26534467002912027No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC53380.24736462602435277No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC53200.24653050027155426No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT48630.22535297421439254No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA48590.22516761293599288No Hit
ACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA47990.2223871937599979No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT47010.21784584243920613No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG45540.21103381545801844No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT45470.21070943322081906No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA42940.19898533236204025No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGC40290.18670514766806245No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG40280.18665880734846252No Hit
TCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACC38620.17896631429487642No Hit
ATCTAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC38440.17813218854207793No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA38240.1772053821500796No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC37810.1752127484072832No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT37430.1734518162624864No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT36030.16696417151849813No Hit
GTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCA35160.16293256371330542No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA35140.1628398830741056No Hit
GCCTCAAGCATGCTATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTT34880.16163503476450775No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT34750.16103261060970886No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA33680.15607419641251782No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT33080.15329377723652285No Hit
CAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAA33080.15329377723652285No Hit
CCCTTATTCCCATGGAACGGGAACAGGCTACACAATAGACACCGTGATCA33080.15329377723652285No Hit
CCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG31400.1455086035437369No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG30950.14342328916174069No Hit
ATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACA30760.14254282308934227No Hit
GTATGATGCACTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGT30380.14078189094454546No Hit
CTTCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACA30240.14013312647014664No Hit
CCTGAAGACAATGAGCCAAGTGCCTATGCACAATTAGATTGCGTTCTGGA30040.13920632007814832No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT30000.13902095879974866No Hit
GAACACATGAGTACTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA29250.13554543482975492No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC29030.13452594779855678No Hit
CACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCTTTGACATTCTTCCT29020.13447960747895685No Hit
GCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC28880.13383084300455803No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC28450.13183820926176162No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA27920.12938217232296606No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT27350.12674077410577086No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG26910.12470180004337454No Hit
CAATTAGATTGCGTTCTGGAGGCTTTGGATAGAATGGATGAGGAACATCC26440.12252380502217848No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA25980.12039215032058233No Hit
TTCATAATCTAGTCGTGCATCCATTCTTAATCTATGGGCCATAGCCTCAA25900.12002142776378301No Hit
TTCCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTA25700.11909462137178468No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG25680.11900194073258484No Hit
ATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCAAGTGC24560.11381182493739424No Hit
ATCTAGTCGTGCATCCATTCTTAATCTATGGGCCATAGCCTCAAGCATGC24460.11334842174139508No Hit
CTGTGAGGCTGCTTGAAACAGACCTGGATGTTCCTCATCCATTCTATCCA24410.11311672014339548No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCACTGTCCAATCGAAAGTCTGTCT24080.11158748959659825No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG24060.11149480895739843No Hit
ATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTTAAAACAGGCCTCAA23500.10889975105980311No Hit
ATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTAAATGT23310.1080192849874047No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG23130.10718515923460621No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23120.10713881891500629No Hit
GTCTACAGTTGTGACCATTAGTGCCTCCATGGCATTCTGTGAGGCTGCTT22960.10639737380140762No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG22620.10482180293501049No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT22390.10375597558421241No Hit
ATCTAATTGTGCATAGGCACTTGGCTCATTGTCTTCAGGTAATGGCCCAT22010.1019950434394156No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA21900.10148529992381652No Hit
GTTCCAGACACTGCATCGTAATCCATTTTCCTCACAGGACCATGTGCTGG21830.10116091768661709No Hit
GAATGGATGAGGAACATCCAGGTCTGTTTCAAGCAGCCTCACAGAATGCC21740.10074385481021784No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT21700.1005584935318182No Hit
GGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGG21620.10018777097501885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC301.0981057E-7120.90788145
GAGCAGA2050.0102.460191
GGCACCC650.0100.446556145
AAACACG8800.0100.414925
GAAACAC8750.0100.158624
AACACGA6500.096.952086
GCACGCA2400.093.55382
ACACGAG6750.093.367757
AGCACGC2450.091.644551
ACGAGCC5750.088.202759
TGACGCC1000.087.05368145
GGTTGCC259.3652547E-487.05368145
CGATAAA750.087.05368145
GCAGCCC1350.085.97894145
CACGAGC7350.084.768178
GCCGACC950.083.99916145
CTAGGCC451.2288565E-680.60526145
GTCGCCC554.3781256E-879.13971145
TAGAAAC11650.078.956662
CACGCAC2850.078.782153
Produced by FastQC (version 0.11.5)