FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9810_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9810_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164973
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC81620.37700239217763915No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC72860.3365399938013084No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA72380.3343228760820574No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT71370.3296576908811334No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC66850.30877983235818646No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC62120.2869319848330672No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT62020.28647008530822327No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA60760.28065015129518933No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA58040.26808648421943365No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA57390.26508413730794794No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC53530.24725481564897117No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC53270.24605387688437685No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT48770.22526839826639872No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA48700.22494506859900795No Hit
ACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA48120.22226605135491298No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT47120.2176470561064734No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG45620.210718563233814No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT45550.21039523356642323No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA42990.1985706057304179No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGC40400.18660740803695935No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG40380.18651502813199056No Hit
TCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACC38690.17870892616212766No Hit
ATCTAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC38500.17783131706492414No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA38340.1770922778251738No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC37890.17501372996337597No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT37500.17321232181648455No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT36140.1669304882786067No Hit
GTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCA35230.16272720260252668No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA35210.16263482269755788No Hit
GCCTCAAGCATGCTATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTT34950.1614338839329636No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT34830.16087960450315084No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA33750.15589108963483608No Hit
CAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAA33170.15321207239074114No Hit
CCCTTATTCCCATGGAACGGGAACAGGCTACACAATAGACACCGTGATCA33140.15307350253328794No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT33130.15302731258080354No Hit
CCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG31470.14535978046839382No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG31030.1433274225590804No Hit
ATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACA30800.1422650536519393No Hit
GTATGATGCACTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGT30420.14050983545753226No Hit
CTTCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACA30290.13990936607523513No Hit
CCTGAAGACAATGAGCCAAGTGCCTATGCACAATTAGATTGCGTTCTGGA30130.13917032683548478No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT30020.13866223735815641No Hit
GAACACATGAGTACTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA29350.1355675105417019No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC29080.13432038182462322No Hit
CACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCTTTGACATTCTTCCT29040.13413562201468565No Hit
GCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC28910.1335351526323885No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC28530.13177993443798144No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA27960.1291471071463709No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT27400.12656046980724472No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG26990.12466668175538448No Hit
CAATTAGATTGCGTTCTGGAGGCTTTGGATAGAATGGATGAGGAACATCC26460.1222186142737115No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA26030.12023244631688247No Hit
TTCATAATCTAGTCGTGCATCCATTCTTAATCTATGGGCCATAGCCTCAA25950.11986292669700731No Hit
TTCCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTA25760.11898531759980378No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG25730.1188467477423506No Hit
ATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCAAGTGC24640.11381204292155145No Hit
ATCTAGTCGTGCATCCATTCTTAATCTATGGGCCATAGCCTCAAGCATGC24510.1132115735392543No Hit
CTGTGAGGCTGCTTGAAACAGACCTGGATGTTCCTCATCCATTCTATCCA24420.11279586396689474No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCACTGTCCAATCGAAAGTCTGTCT24130.11145635534484727No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG24090.11127159553490967No Hit
ATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTTAAAACAGGCCTCAA23540.1087311481482679No Hit
ATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTAAATGT23330.1077611591460956No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG23190.10711449981131406No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23190.10711449981131406No Hit
GTCTACAGTTGTGACCATTAGTGCCTCCATGGCATTCTGTGAGGCTGCTT23070.1065602203815013No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG22670.10471262228212545No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT22410.10351168351753118No Hit
ATCTAATTGTGCATAGGCACTTGGCTCATTGTCTTCAGGTAATGGCCCAT22060.10189503518057731No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA21930.10129456579828017No Hit
GTTCCAGACACTGCATCGTAATCCATTTTCCTCACAGGACCATGTGCTGG21840.1008788562259206No Hit
GAATGGATGAGGAACATCCAGGTCTGTTTCAAGCAGCCTCACAGAATGCC21810.10074028636846741No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT21740.10041695670107663No Hit
GGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGG21670.10009362703368586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC306.475602E-10145.1235145
GGCACCC700.0124.39157145
GAAACAC8250.0111.510214
AAACACG8750.0105.966055
AACACGT2650.098.415076
GAGCAGA2600.097.503331
AACGGCC900.096.749145
ACACGTG2700.096.592577
AACACGA7100.093.871646
TAGAAAC11050.088.49452
ACACGAG7550.088.276667
GCAGCCC1400.088.110695145
GCGAGAA502.2566383E-887.0741145
ACGAGCC6000.086.941349
CACGTGC3000.086.935328
GCACGCA2100.086.231412
CACGAGC7750.086.0005348
TGACGCC608.240022E-1084.65538145
GCCGACC608.240022E-1084.65538145
GGGCCCC353.4236946E-582.92771145
Produced by FastQC (version 0.11.5)