FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9722_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9722_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425577
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG128940.5315848558920208No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG87830.3620994097486907No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT79860.32924124857714265No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC78870.3251597454956079No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG70520.2907349467776121No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC69000.2844683965918212No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG62920.25940219584865787No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT54120.22312216845723717No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG50720.20910488514691555No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA49110.20246728922643972No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT48570.20024101481832982No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC46750.19273764551692238No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA44760.18453341204999882No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC43560.17958613558753236No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA43270.17839054377576963No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC40970.1689082638893756No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC39790.16404344203461693No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC39030.1609101669417215No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT36970.15241734234782076No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT36890.15208752391698965No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT36840.15188138739772022No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG35950.14821215735472426No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA35010.14433679079245887No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA34580.14256401672674174No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA33460.13794655869510636No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG33260.13712201261802862No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA32540.13415364674054875No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT31700.13069055321682221No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA31370.12933005218964394No Hit
TGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA30480.12566082214664798No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC30400.1253310037158169No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT30220.12458891224644694No Hit
GTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG29420.12129072793813597No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC29290.12075477298803543No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG29100.11997145421481156No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC28900.11914690813773383No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA27760.1144469954983907No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA27490.11333385829433575No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG27080.11164353883632637No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG26240.10818044531259985No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT26060.10743835384322986No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC25920.10686117158927545No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT25380.10463489718116556No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA25370.10459366987731168No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAAT24740.10199634973451677No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT24730.10195512243066289No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA24530.10113057635358513No Hit
TCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT24330.10030603027650742No Hit
GCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGT24290.10014112106109185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG100.0070735267145.123145
TGACGCC1250.081.268875145
AGCGAAA7450.080.695351
ATCGACC502.2956392E-672.5615145
CGTATCC350.003555154262.195568145
CGAGTAA350.003555154262.195568145
CGACTAA350.003555154262.195568145
GCTTATA1200.060.3594741
AAGGGTG12800.055.463275
CAAGGGT14600.053.584894
GCGAAAG11850.051.3437542
TAGAAAC19400.048.909842
CGAAAGC12650.048.6713033
CGGGGGG752.5389088E-548.374336145
AGCGTAA450.00960854148.374332145
CTTATAA901.3166464E-648.374332145
GACGCCC450.00960854148.374332145
CAAGGTA11250.048.2925534
TATATTC4200.048.289573
CACTAAC1203.6561687E-948.287582
Produced by FastQC (version 0.11.5)