FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9693_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9693_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799027
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA67060.3727570514505897No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT64140.3565260554733197No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT60730.33757136496561757No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT40100.2228982666741522No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC37960.21100294770450917No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA37430.20805691076342933No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT31670.17603960363018453No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA30280.16831320486018275No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT30070.1671459072042832No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG29770.1654783391244267No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA29740.16531158231644105No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA29000.16119824771946167No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT28290.15725166993046796No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG27730.15413887618140248No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA26910.14958085676312807No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG26860.14930292874981865No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC26580.1477465318752859No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT26540.14752418946463838No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA26220.1457454501794581No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC26090.14502283734485363No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC25730.14302175564902583No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT25240.14029806111859355No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT25170.13990896189996035No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT24940.13863049303873706No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT24150.13423923042844826No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC23620.13129319348736845No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA23330.12968121101017382No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT23040.1280692285329792No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA22860.12706868768506532No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC22440.12473409237326621No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA22260.12373355152535233No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC22150.1231221098960716No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC22110.12289976748542407No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT21750.12089868578959626No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG21720.12073192898161061No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG21310.1184529192724734No Hit
GTATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC20870.11600715275535053No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC20630.11467309829146534No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG20570.11433958467549402No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT20140.11194940376103305No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA20030.11133796213175233No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG19980.11106003411844292No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG19520.10850309639599627No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT19430.10800282597203932No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG19280.10716904193211108No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT19270.1071134563294492No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA18990.10555705945491647No Hit
TATCTAGACTGAGGGATATATCTGTTCCTGCTGGTTTCTCCAATTTTGAA18940.10527913144160704No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC18710.10400066258038373No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA18520.10294453612980795No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC18490.1027777793218223No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA18480.10272219371916042No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG18200.10116579684462768No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA18170.10099904003664202No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG100.00707518145.11063145
CGTTACC151.226436E-4145.11061145
CGCACCC351.893568E-9124.38052145
ACACGTG1050.0124.204217
AACACGT1100.0118.555266
GCACGCA3000.0108.6665952
CTAGGCC553.6379788E-12105.534996145
CACGCAC3100.0105.1612243
AGCACGC3150.0103.4920041
AAACACG3650.0103.211335
CGATAAA501.9463187E-10101.57743145
ACACGAG2450.0100.5462657
CGACGGG750.096.74042145
CAAGCCC451.0863005E-896.74041145
GCTGCCC1300.094.88001145
ATAAGCC700.093.28539145
AACGGCC406.091741E-790.69414145
CACGAGC2750.089.577588
GCCGACC502.2577296E-887.06637145
AACACGA2850.086.4321066
Produced by FastQC (version 0.11.5)