FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9609_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9609_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722926
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG60910.35352650084797604No Hit
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG60480.3510307465323525No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC60160.3491734409951443No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC58410.3390163013385369No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA54970.3190502668135486No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA49070.28480619597127216No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG46490.2698316700775309No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT43400.25189706348386404No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG40720.23634212960974527No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT36630.21260344321230282No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG35000.20314279313214845No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT34340.1993121004616565No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC34140.19815128450090136No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG32720.18990949117953992No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA32360.1878200224501807No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC32330.1876459000560674No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT29740.17261333336428844No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC29060.16866655909772096No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT28840.16738966154089033No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG28440.16506802961938005No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT27440.15926394981560438No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA27340.15868354183522682No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAGTGCATGTGTC25940.1505578301099409No Hit
TAGTAGCACTGCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAA25470.14782991260216632No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC25390.14736558621786428No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG25200.14626281105514688No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT23970.1391237928965028No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC23770.13796297693574766No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAGCA23510.136453916186766No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT22520.1307078771810281No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC22380.1298953060084995No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTT22350.12972118361438623No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA21930.12728347009680044No Hit
ATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA21690.1258904909438943No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA21440.12443947099295037No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA20510.11904167677543899No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC20460.1187514727852502No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC20390.1183451871989859No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC19960.11584943288336237No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG19520.11329563776970109No Hit
ATTGAATACTGACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGAAC19420.1127152297893235No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA19330.11219286260698369No Hit
CTATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGA19190.11138029143445512No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA19180.11132225063641736No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA19090.11079988345407754No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG18870.10952298589724689No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT18750.10882649632079382No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC18740.10876845552275606No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG18590.10789784355218969No Hit
CCTTTGGGGGACTCCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAA18400.10679506838947232No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG18080.10493776285226411No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT17940.10412519167973552No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA17780.10319653891113141No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC17700.10273221252682936No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT17300.10041058060531909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4150.0113.445761
GCTGACC1800.092.71454145
GAGCGAA1050.089.676181
GGTCTTT651.4551915E-1189.30364145
TCACGCC1000.087.071045145
CGACTCC850.085.36378145
CGAAAGC5900.079.798913
GAAGCCC1300.078.140686145
TGACGCC851.8189894E-1276.8274145
CGTCACC950.076.37811145
TAACTTT300.001929720972.55921145
GGACTTT502.2957865E-672.559204145
TTATATT12600.071.857992
CAGTACG11050.070.148789
TATATTC13000.069.646983
AGACGCC554.0432114E-665.96291145
GACGCAA554.0432114E-665.96291145
GGGTCAC451.18928816E-464.49707145
GTGGGCC1700.064.022835145
ATATTCA14200.063.763174
Produced by FastQC (version 0.11.5)