FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9504_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9504_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375860
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTCTCGTTACAGTAACT44730.3251057520387249No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA30580.2222609858561191No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT27980.2033637143314No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA26530.19282485136569127No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA26240.19071707877254954No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC26200.1904263515183231No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC23420.17022080734958497No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA22430.16302530780748042No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT21450.15590249007893245No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT21360.15524835375692295No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG21300.15481226287558325No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT21270.15459421743491344No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA21050.15299521753666798No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC18980.13795008213044932No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA18830.13685985492710015No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT18610.13526085502885468No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA18360.13344380968993938No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG18270.13278967336792988No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG18160.13199017341880714No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATATATCACCCAGG18130.1317721279781373No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG17890.13002776445277864No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT17740.12893753724942944No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT17510.12726585553762737No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA17040.12384981030046661No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA16760.12181471952088148No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC16700.12137862863954181No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC16460.11963426511418312No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA16360.118907446978617No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTT16340.11876208335150379No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA16260.11818062884305089No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC16060.11672699257191865No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC15660.11381972002965418No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT15610.11345631096187112No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA15510.112729492826305No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT15470.11243876557207855No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATAT15120.10989490209759716No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG15120.10989490209759716No Hit
TTCTAATACTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACC14850.10793249313156862No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT14790.10749640225022894No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT14470.10517058421641737No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT14420.1048071751486343No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC14360.10437108426729463No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG14250.10357158431817191No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT13830.10051894814879421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCC100.0070785535145.08624145
CGAAGGT100.0070785535145.08624145
ACGTGCG100.007104144.912229
AGCGCAA100.007108633144.880621
TGACGCC404.796675E-9108.81468145
GAAACAC4600.0108.664424
AACACGT2100.0106.951256
ATAAGCC352.7525311E-7103.63304145
CACGTGC2250.099.8284158
ACACGAG3000.099.0233467
ACGTGCA1250.098.540319
AACACGA3050.097.392946
CGCACCC301.5949568E-596.72417145
ACGTGCC900.096.608159
AAACACG5200.096.126215
ACGAGCC2000.090.570149
CACGAGC3250.089.176758
ACAACGC2050.088.36118
GCAGCCC259.3653495E-487.05175145
TAACACG259.416908E-486.931535
Produced by FastQC (version 0.11.5)