FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9497_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9497_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631467
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT53810.3298258561159987No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA45020.27594796584914066No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA36650.22464444576568207No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT35480.21747298596906955No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG31320.1919744622477807No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG30480.18682572188098195No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT28960.17750895359820335No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA28680.17579270680927042No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT28020.17174726794964285No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC27950.17131820625240962No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA27270.16715017833642973No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG26650.16334991758950687No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA24410.14961994327804362No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG22730.1393224625444462No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG22660.13889340084721297No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA22120.1355834963256995No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA22040.1350931401002901No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT21480.1316606465224243No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC21080.1292088653953773No Hit
GTATTAAAGTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA20920.1282281529445585No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA20780.12737002955009205No Hit
ACCTAAAAGAGGTTATTGTTGTGTTCAGTGCCGTTGCAGCAGGTTGGTTT20010.12265035088052653No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT19850.12166963842970774No Hit
ATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAAATCCC19650.12044374786618424No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA18510.11345617165410025No Hit
ATATTAAACATTCCCATCATCATCCCAGGCGACAAAGATGCAGTTCCTTC18490.11333358259774792No Hit
CTTATGGGATGGACTGCAATCTTCTGATGATTTTGCTCTATTTGTTAATG18050.11063662335799622No Hit
ATTAGATACAAATCCATCTCTGTAGAACATGCTTGTAAATTCAAACATTC17820.10922684920994419No Hit
CTTTTGATGTATTCCACTTTGGTTATTTTGTCTTTTACCTTCATTGGTAT17800.10910426015359183No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT17350.10634600638566394No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC17120.10493623223761192No Hit
TGTTTACTCCCAATAATTTACATGTTCGGTAAAAGTCGTTTATTCCTTCC16940.10383293073044075No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT16820.10309739639232667No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA16790.10291351280779815No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC16560.1015037386597461No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT16520.10125856054704141No Hit
CCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAAAAGCTAAA16360.10027784809622259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTAG100.0070764145.10184145
CGTGCCC151.2267106E-4145.10184145
AGGCCCC100.0070764145.10184145
GAGCCGG100.0070764145.10184145
GCCACCC100.0070764145.10184145
CGCACCC403.274181E-11126.9641145
GGTACCC301.0974145E-7120.9182145
CGGTCCC203.8557147E-4108.82638145
TAGAAAC5300.0107.9530262
AACACGT1700.0106.515946
AAACACG5250.0106.228645
AGTGGGG352.7510032E-7103.64417145
ATAAGCC352.7510032E-7103.64417145
ACACGTG1850.097.885527
AACACGA3900.096.574456
GAAACAC5800.096.152294
AACGGCC406.093687E-790.688644145
TGGTCCC406.093687E-790.688644145
GTAACAA4800.090.5334
ACACGAG4200.089.681777
Produced by FastQC (version 0.11.5)