FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9475_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9475_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346579
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGA10980.31681088582978195No Hit
GTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGTGCTCCAGCTT9810.2830523488151331No Hit
GTATACGTACACAAATATCTTTGGAGGCGCAATATAGTTTAAAACAACAC8870.25593010540165445No Hit
GGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATG8360.24121484567732032No Hit
GGTCCGAGCAGCTGCGCGCCTTCAGTGACTATGGCAACCACACTCACGCC8360.24121484567732032No Hit
CATATAGGGTGGGTGGAAGAAATGGAGCACTGGCGAACCAATCTGTATGG8180.23602122459814356No Hit
GTATAAAGCATTCGGCCAGCACTGAAAATACCGCTATTACAGCCAGACAT7980.2302505345101694No Hit
GCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGT7910.22823079297937843No Hit
CATATAGGTTGCCTGAACAGCATTTCCAACTGGCCCAAACGGTGATTGAA7790.22476837892659393No Hit
GTATATGCTCAACAGCTGGTTTTCCTTCCAAAGACTTAAGCTTTAGCTTG7530.21726648181222752No Hit
GCACTGGTCCGAGCAGCTGCGCGCCTTCAGTGACTATGGCAACCACACTC7530.21726648181222752No Hit
GTACATTGCTTGGTGATACAGACAAGCCAACTGCTCTGAAAGCACAGAGG7420.21409260226384172No Hit
CCCATAAACCTCGAGAATATAAGCATAGTCTCCGCCCGATTTGGAGATTG7380.21293846424624688No Hit
GTCCAAGTTTCTGTTACGCTTTCATTTCTAACTCTGTCAGTCCTTGATTA7190.20745630866267142No Hit
AAAGCAGGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTC6860.19793467001751405No Hit
GGGTGTACAGTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCG6540.18870156587675538No Hit
CTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCCGTGGGATC6420.18523915182397085No Hit
GTCGTAGGGATGTCAGAAATTATTGCGGTTGGAGCGTATATGAAGTACTG6350.1832194102931799No Hit
ATATAAAGGCACACCATTACGAGAAATCTTCGTAAAGAATTTTGGCGCTT6310.18206527227558505No Hit
GTACTGGTTTGAGTCTGTGATCAGTCCAAGTTTCTGTTACGCTTTCATTT6300.18177673777118636No Hit
ATATTAATCTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCC6200.17889139272719928No Hit
CTTTAAGGTGATCCTTGCTTTTGGCTTTTTACCTGTACATTGCTTGGTGA6080.17542897867441476No Hit
CCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGCTGCACTGGTCC5940.17138949561283284No Hit
CACTCACGCCGTGGCCCTGCTGCGGGAGAAGGTGTACGTGCCCCCCGGGC5910.17052389209963673No Hit
ATATAGAAGATTAATATACGCCAAATAATACTTTGGATCGCGCTTGTTAA5710.16475320201166255No Hit
GTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGC5550.16013664994128324No Hit
GACAAAGATGTGAGCAATGTTGTCGGAGAAGAAACTGTTGCTTTACAAAG5490.15840544291489098No Hit
AAATAATACTTTGGATCGCGCTTGTTAACGTTTTTTGCGGATTTTTCGCT5480.15811690841049228No Hit
ATAATAAATAATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATA5420.15638570138410002No Hit
GCCTGAATCAGTGAATGAATGTGAGCTGTGGAGGTCCAAACACCCCAAAC5420.15638570138410002No Hit
CTATAGAGTAGAGGGCCCTCCGGACCTAGCTCTCGTACGAGTTTCTGAGC5380.1552315633665052No Hit
GTATGGATGATGGGCCTATCTACCTCGTTCTCGGCGAACCAACCTAACTG5200.15003794228732845No Hit
GTAAGAAGGGGAAGAGGCTGACGTACCCCAGTGCGTTGACAAACTGCAGC5180.14946087327853103No Hit
TCTTTAAGGTGATCCTTGCTTTTGGCTTTTTACCTGTACATTGCTTGGTG5160.1488838042697336No Hit
GTATTATTTGGCGTATATTAATCTTCTATATTGGTGCTATTTTTGTAATT5100.14715259724334134No Hit
CCTTCAGTGACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAG5010.14455578670375296No Hit
CTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATGGCACCACTCC4840.13965070012897493No Hit
ATGTGAAACAAGCCATCAACTATCATTGCTTGGCTTGTCATGAGGAAGAG4840.13965070012897493No Hit
TACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCAT4820.1390736311201775No Hit
CACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGCTGCACTGGTCCGA4790.1382080276069814No Hit
AAGCAGGAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCT4780.13791949310258267No Hit
GTCTTGCTCTGTCCCCCAGGCTGGAGTGCAGGCACACCACCTCGGCTCAC4680.1350341480585956No Hit
CCTTAAAGAGGAAGAGATGTCAGGGATGAGCTCAGAGGAAAATAGGGATT4660.13445707904979817No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4510.13012906148381756No Hit
ACTCTATAGTTGGCATATCTGGTTCGCCGGCATGCATGGAAGAACCATTC4420.12753225094422918No Hit
AGCGAAAGCAGGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTC4410.12724371643983046No Hit
GTGCAGTAAGAAGGGGAAGAGGCTGACGTACCCCAGTGCGTTGACAAACT4410.12724371643983046No Hit
GTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGTGCT4390.12666664743103304No Hit
CCCCAAACCACCTCATCCCTTGAACGAATCCGCGTTTCACAACGAGGCTC4370.12608957842223562No Hit
GGTTTGAGTCTGTGATCAGTCCAAGTTTCTGTTACGCTTTCATTTCTAAC4350.12551250941343822No Hit
GACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAGAAGGTGTA4330.12493544040464079No Hit
TCTTAATACTCTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATG4300.12406983689144467No Hit
CTCATGTCCCTCAGGATGTGCTCCAGCTTGGGCGAGTCGGGCGTCAGGAT4210.12147302635185629No Hit
CTCATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAACACCTGCAG4190.12089595734305887No Hit
GCAATGTACAGGTAAAAAGCCAAAAGCAAGGATCACCTTAAAGAGGAAGA4150.11974181932546404No Hit
CTGTTACACTGACTATCTGTCTGAAGTAGTTTGACTGCTTTTATTGTTGT4150.11974181932546404No Hit
GTACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCA4100.11829914680347049No Hit
GCTTTATACATTGGGAGTGAATGGTCAAGCGCCAAAATTCTTTACGAAGA4100.11829914680347049No Hit
ATCCAGCACCATTCAATGGACGGGTCCGTCTGTACTGCTTGCTTATGCAA4080.11772207779467307No Hit
AGCGAAAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCA4040.11656793977707824No Hit
GAGCAAGACTTCATCTCAAATAATAATAATAAATAATATAAAAAGGGAAT3950.11397112923748987No Hit
CCCTCAGGATGTGCTCCAGCTTGGGCGAGTCGGGCGTCAGGATGTGCAGT3940.11368259473309116No Hit
TCTCAAAGGGCCCCGGGTCTCTCCAGCTGCCCCGCCAACCTCACCCTCCC3940.11368259473309116No Hit
GATAAGGAATTTAGAGACAGGCTCCCAGCTCTGGGTGAATGACAGTAAAT3930.11339406022869244No Hit
GGAGGGGGAAGAGCGAGCTTCCTATGGAATGGTTTCAGTTCCCAATTCGG3880.11195138770669891No Hit
GGTAATTCCAGAGCATCCACCTTTCTTCATTTACTGTCATTCACCCAGAG3870.1116628532023002No Hit
CTTCTTAAGAGCATCTTTTGACATGCGTGTCACCTCCTTTCAAAAGTAGC3870.1116628532023002No Hit
CCCTATGGCCTGTTGCAGCATGGACTAGCGCTTTTTCTTCTGATTGAGTT3870.1116628532023002No Hit
GAATGAATGTGAGCTGTGGAGGTCCAAACACCCCAAACCACCTCATCCCT3780.10906604266271182No Hit
ATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATAGGAGGGGGAA3780.10906604266271182No Hit
GTCCTTGATTATTTGGCAAGACGAGGCTTGCTTCAACCTCCTGACATGAA3780.10906604266271182No Hit
AGCATATACTGATCCCCGAGAGCCTCACATCAAATTTGCTAAGAAGGACC3760.1084889736539144No Hit
AATGTGAGCTGTGGAGGTCCAAACACCCCAAACCACCTCATCCCTTGAAC3760.1084889736539144No Hit
GGGTTAGAGAGTATTAAGAAAAACCAGGCATAACCCGCCCGGTCCTTTGT3730.10762337014071828No Hit
GTGATACAGACAAGCCAACTGCTCTGAAAGCACAGAGGTAATTCCAGAGC3700.10675776662752215No Hit
GTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCGTGTCCTCGC3680.10618069761872473No Hit
CATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAACACCTGCAGGT3660.10560362860992732No Hit
TTCAGTGACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAGAA3630.10473802509673119No Hit
TCCCTGACATCTCTTCCTCTTTAAGGTGATCCTTGCTTTTGGCTTTTTAC3610.10416095608793377No Hit
GCTTAGGGGCATTACGCCCTGAGCCGACCGAGCGCACCAGGCGTGCCACG3590.10358388707913636No Hit
CTTAAGAAGACCGTTAAAAAAATAGGGGAACAATCCGTGCCTATCATTGC3580.10329535257473765No Hit
GCCTAATAGTATCAGTATCTATTACCAAGTGATGAGAGTAAGGCAAGCTC3560.10271828356594023No Hit
AGCAGGGAGAGACCCATAAACCTCGAGAATATAAGCATAGTCTCCGCCCG3560.10271828356594023No Hit
CCCTCCCACTCATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAAC3560.10271828356594023No Hit
GTTTACTACCATCTTGTACCCCCGTAGGGCCCCTGCTGCACCATTGTATG3530.1018526800527441No Hit
AAATAATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATAGGAGG3510.1012756110439467No Hit
ATCTAACCTTTGGGCACATGGCGGCTTTTTTACAGGTGGCTGGTCAGGAT3490.10069854203514927No Hit
GACGTACCCCAGTGCGTTGACAAACTGCAGCTTAGGGGCATTACGCCCTG3470.10012147302635185No Hit
GTATACTGCCGGATCGGTCGAAATCAGGCTGCGGCTATGTGCTGGAAAAG3470.10012147302635185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGGG202.1222386E-6145.08746145
TGACCAA100.0070761037145.08746145
AAAGGCC100.0070761037145.08746145
CGAAGGG100.0070761037145.08746145
TCCGTCC151.2263663E-4145.08745145
TATAGTA100.007112868144.836232
GGCGATA151.2355742E-4144.815321
GCAGCCC301.09621396E-7120.90621145
TTACTTT203.8546388E-4108.8156145
GCAGGGT203.876856E-4108.658528
GCGATAT203.881311E-4108.627172
CGAAAGC3900.0102.12813
GCGAAAG4050.098.345582
CATTACC301.5931964E-596.72497145
TAGGGGG259.359078E-487.05247145
GAGCACC259.359078E-487.05247145
CAGGGTT259.40755E-486.939369
TGAGACA259.412947E-486.926828
CGATATG259.4237487E-486.901733
GAAGTGG353.4238383E-582.90711145
Produced by FastQC (version 0.11.5)