FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9475_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9475_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347789
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGA11020.3168587850679579No Hit
GTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGTGCTCCAGCTT9850.28321769808705854No Hit
GTATACGTACACAAATATCTTTGGAGGCGCAATATAGTTTAAAACAACAC8910.2561898162391565No Hit
GGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATG8390.24123822202542347No Hit
GGTCCGAGCAGCTGCGCGCCTTCAGTGACTATGGCAACCACACTCACGCC8390.24123822202542347No Hit
CATATAGGGTGGGTGGAAGAAATGGAGCACTGGCGAACCAATCTGTATGG8220.23635020084016456No Hit
GTATAAAGCATTCGGCCAGCACTGAAAATACCGCTATTACAGCCAGACAT8010.2303120570230801No Hit
GCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGT7920.22772428110147247No Hit
CATATAGGTTGCCTGAACAGCATTTCCAACTGGCCCAAACGGTGATTGAA7810.224561443863952No Hit
GCACTGGTCCGAGCAGCTGCGCGCCTTCAGTGACTATGGCAACCACACTC7560.21737317741504186No Hit
GTATATGCTCAACAGCTGGTTTTCCTTCCAAAGACTTAAGCTTTAGCTTG7540.21679811609912908No Hit
GTACATTGCTTGGTGATACAGACAAGCCAACTGCTCTGAAAGCACAGAGG7440.21392280951956505No Hit
CCCATAAACCTCGAGAATATAAGCATAGTCTCCGCCCGATTTGGAGATTG7380.2121976255718266No Hit
GTCCAAGTTTCTGTTACGCTTTCATTTCTAACTCTGTCAGTCCTTGATTA7210.2073096043865677No Hit
AAAGCAGGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTC6880.19782109267400638No Hit
GGGTGTACAGTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCG6550.188332580961445No Hit
CTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCCGTGGGATC6430.18488221306596817No Hit
GTCGTAGGGATGTCAGAAATTATTGCGGTTGGAGCGTATATGAAGTACTG6360.1828694984602733No Hit
GTACTGGTTTGAGTCTGTGATCAGTCCAAGTTTCTGTTACGCTTTCATTT6310.1814318451704913No Hit
ATATAAAGGCACACCATTACGAGAAATCTTCGTAAAGAATTTTGGCGCTT6310.1814318451704913No Hit
ATATTAATCTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCC6210.17855653859092727No Hit
CTTTAAGGTGATCCTTGCTTTTGGCTTTTTACCTGTACATTGCTTGGTGA6080.17481864003749398No Hit
CCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGCTGCACTGGTCC5950.17108074148406074No Hit
CACTCACGCCGTGGCCCTGCTGCGGGAGAAGGTGTACGTGCCCCCCGGGC5930.17050568016814793No Hit
ATATAGAAGATTAATATACGCCAAATAATACTTTGGATCGCGCTTGTTAA5710.16418000569310703No Hit
GTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGC5580.16044210713967377No Hit
GACAAAGATGTGAGCAATGTTGTCGGAGAAGAAACTGTTGCTTTACAAAG5510.15842939253397895No Hit
AAATAATACTTTGGATCGCGCTTGTTAACGTTTTTTGCGGATTTTTCGCT5490.15785433121806614No Hit
ATAATAAATAATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATA5440.1564166779282841No Hit
GCCTGAATCAGTGAATGAATGTGAGCTGTGGAGGTCCAAACACCCCAAAC5430.1561291472703277No Hit
CTATAGAGTAGAGGGCCCTCCGGACCTAGCTCTCGTACGAGTTTCTGAGC5400.1552665552964585No Hit
GTATGGATGATGGGCCTATCTACCTCGTTCTCGGCGAACCAACCTAACTG5210.1498034727952868No Hit
GTAAGAAGGGGAAGAGGCTGACGTACCCCAGTGCGTTGACAAACTGCAGC5180.1489408808214176No Hit
TCTTTAAGGTGATCCTTGCTTTTGGCTTTTTACCTGTACATTGCTTGGTG5160.1483658195055048No Hit
GTATTATTTGGCGTATATTAATCTTCTATATTGGTGCTATTTTTGTAATT5100.14664063555776635No Hit
CCTTCAGTGACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAG5040.14491545161002792No Hit
CTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATGGCACCACTCC4840.13916483845089983No Hit
ATGTGAAACAAGCCATCAACTATCATTGCTTGGCTTGTCATGAGGAAGAG4840.13916483845089983No Hit
TACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCAT4830.1388773077929434No Hit
CACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGCTGCACTGGTCCGA4800.1380147158190742No Hit
AAGCAGGAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCT4790.13772718516111782No Hit
GTCTTGCTCTGTCCCCCAGGCTGGAGTGCAGGCACACCACCTCGGCTCAC4690.13485187858155376No Hit
CCTTAAAGAGGAAGAGATGTCAGGGATGAGCTCAGAGGAAAATAGGGATT4660.13398928660768455No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4520.1299638573962949No Hit
ACTCTATAGTTGGCATATCTGGTTCGCCGGCATGCATGGAAGAACCATTC4420.12708855081673084No Hit
GTGCAGTAAGAAGGGGAAGAGGCTGACGTACCCCAGTGCGTTGACAAACT4420.12708855081673084No Hit
AGCGAAAGCAGGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTC4410.12680102015877442No Hit
GTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGTGCT4390.1262259588428616No Hit
CCCCAAACCACCTCATCCCTTGAACGAATCCGCGTTTCACAACGAGGCTC4370.1256508975269488No Hit
GGTTTGAGTCTGTGATCAGTCCAAGTTTCTGTTACGCTTTCATTTCTAAC4360.1253633668689924No Hit
GACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAGAAGGTGTA4340.12478830555307958No Hit
TCTTAATACTCTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATG4300.12363818292125398No Hit
CTCATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAACACCTGCAG4220.12133793765760274No Hit
CTCATGTCCCTCAGGATGTGCTCCAGCTTGGGCGAGTCGGGCGTCAGGAT4210.12105040699964634No Hit
GCAATGTACAGGTAAAAAGCCAAAAGCAAGGATCACCTTAAAGAGGAAGA4160.11961275370986431No Hit
CTGTTACACTGACTATCTGTCTGAAGTAGTTTGACTGCTTTTATTGTTGT4150.11932522305190792No Hit
ATCCAGCACCATTCAATGGACGGGTCCGTCTGTACTGCTTGCTTATGCAA4120.1184626310780387No Hit
GTACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCA4110.11817510042008228No Hit
GCTTTATACATTGGGAGTGAATGGTCAAGCGCCAAAATTCTTTACGAAGA4110.11817510042008228No Hit
AGCGAAAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCA4050.11644991647234387No Hit
CCCTCAGGATGTGCTCCAGCTTGGGCGAGTCGGGCGTCAGGATGTGCAGT3950.11357460989277982No Hit
GAGCAAGACTTCATCTCAAATAATAATAATAAATAATATAAAAAGGGAAT3950.11357460989277982No Hit
TCTCAAAGGGCCCCGGGTCTCTCCAGCTGCCCCGCCAACCTCACCCTCCC3940.11328707923482341No Hit
GATAAGGAATTTAGAGACAGGCTCCCAGCTCTGGGTGAATGACAGTAAAT3940.11328707923482341No Hit
GGTAATTCCAGAGCATCCACCTTTCTTCATTTACTGTCATTCACCCAGAG3890.1118494259450414No Hit
GGAGGGGGAAGAGCGAGCTTCCTATGGAATGGTTTCAGTTCCCAATTCGG3880.11156189528708498No Hit
CCCTATGGCCTGTTGCAGCATGGACTAGCGCTTTTTCTTCTGATTGAGTT3880.11156189528708498No Hit
CTTCTTAAGAGCATCTTTTGACATGCGTGTCACCTCCTTTCAAAAGTAGC3870.11127436462912857No Hit
GTCCTTGATTATTTGGCAAGACGAGGCTTGCTTCAACCTCCTGACATGAA3810.10954918068139016No Hit
GAATGAATGTGAGCTGTGGAGGTCCAAACACCCCAAACCACCTCATCCCT3780.10868658870752093No Hit
ATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATAGGAGGGGGAA3780.10868658870752093No Hit
AGCATATACTGATCCCCGAGAGCCTCACATCAAATTTGCTAAGAAGGACC3770.10839905804956454No Hit
AATGTGAGCTGTGGAGGTCCAAACACCCCAAACCACCTCATCCCTTGAAC3760.10811152739160813No Hit
GGGTTAGAGAGTATTAAGAAAAACCAGGCATAACCCGCCCGGTCCTTTGT3740.10753646607569531No Hit
GTGATACAGACAAGCCAACTGCTCTGAAAGCACAGAGGTAATTCCAGAGC3710.1066738741018261No Hit
GTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCGTGTCCTCGC3710.1066738741018261No Hit
CATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAACACCTGCAGGT3670.1055237514700005No Hit
TTCAGTGACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAGAA3640.10466115949613126No Hit
TCCCTGACATCTCTTCCTCTTTAAGGTGATCCTTGCTTTTGGCTTTTTAC3640.10466115949613126No Hit
GCTTAGGGGCATTACGCCCTGAGCCGACCGAGCGCACCAGGCGTGCCACG3600.10351103686430566No Hit
CTTAAGAAGACCGTTAAAAAAATAGGGGAACAATCCGTGCCTATCATTGC3580.10293597554839286No Hit
GCCTAATAGTATCAGTATCTATTACCAAGTGATGAGAGTAAGGCAAGCTC3560.10236091423248003No Hit
AGCAGGGAGAGACCCATAAACCTCGAGAATATAAGCATAGTCTCCGCCCG3560.10236091423248003No Hit
CCCTCCCACTCATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAAC3560.10236091423248003No Hit
AAATAATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATAGGAGG3530.10149832225861083No Hit
GTTTACTACCATCTTGTACCCCCGTAGGGCCCCTGCTGCACCATTGTATG3530.10149832225861083No Hit
ATCTAACCTTTGGGCACATGGCGGCTTTTTTACAGGTGGCTGGTCAGGAT3500.1006357302847416No Hit
GACGTACCCCAGTGCGTTGACAAACTGCAGCTTAGGGGCATTACGCCCTG3480.1000606689688288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACC450.0129.05096145
ATTGGGG203.844639E-4108.88674145
GAGCACC352.7373426E-7103.70167145
GCGAAAG3500.0103.448192
CGAAAGC3650.099.196893
ATCAAAA301.5880392E-596.78822145
GCAGCCC451.0801159E-896.78822145
GAGCGAA259.4260415E-486.896471
CAAGATT353.4544573E-582.758554
ACTAAAG651.5752448E-977.984023
CTTAAAA300.001925052372.59116145
AATGGGG300.001925052372.59116145
AAGCTCC300.001925052372.59116145
CACGCCC502.2859313E-672.591156145
ATTCCAC406.686929E-572.4345558
GCAGGCT406.686929E-572.4345558
TAAAGAT702.8321665E-972.4137345
CCAAGAT451.1998157E-464.367763
ATTGGAT451.1998157E-464.367764
AGCAGGC3300.063.636313
Produced by FastQC (version 0.11.5)