FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9464_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9464_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576092
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG66400.4212952035794865No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT43910.27860048778878393No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG43890.2784735916431274No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC42240.26800465962646847No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC37170.2358364867025529No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG31600.2004959101372255No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG28100.17828908464734292No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT27950.17733736355491939No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA27340.173467031112397No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA26580.16864497757745106No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC25740.16331533945987925No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG24830.15754156483250978No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT24500.15544777842917798No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG24100.15290985551604855No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG23680.15024503645726264No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA23510.14916641921918264No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA22250.1411719620428249No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA22150.14053748131454255No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC22000.13958576022211902No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA21990.13952231214929078No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC20720.1314644069001048No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA20440.12968786086091422No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT20260.12854579555000595No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC20180.12803821096738008No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAGACACAGGGAACTGAAAGACT20170.12797476289455184No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC19580.12423132659768592No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT19300.12245478055849532No Hit
TTACTAAGGAGTGCCATAGGCCAAATTTCAAGGCCGATGTTCTTGTATGT18950.12023409800950705No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC18790.11921892884425528No Hit
CCTTAGTAACATATCTCCTATCTCAAGGACACAATATTTCTCCCATTTAT18680.11852100004314468No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC18660.11839410389748821No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA18630.11820375967900351No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT18620.11814031160617527No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG18550.11769617509637761No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA18470.11718859051375173No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT18460.11712514244092351No Hit
TTCTTAAACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT17850.1132548099984011No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC17730.11249343312446228No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA17710.1123665369788058No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA17580.11154171203203873No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA17490.11097067937658463No Hit
GTGTTAATGTATACCCCTTTCATTATGTATTCAGTAGCTCTACAATGGGA17260.1095113737015352No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG17080.10836930839062694No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCGATTTGTACTCCTCTAGTT17000.10786172380800106No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA16880.10710034693406222No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA16830.10678310656992104No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC16510.1047527682394175No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG16440.10430863172961984No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT16270.10323001449153983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGCC202.1228207E-6145.11197145
CCCCGCC100.007074899145.11197145
GCTGACC1550.0126.38785145
ATCGACC203.8546187E-4108.83398145
CGACTCC553.6379788E-12105.53599145
GAAGCCC950.099.28714145
CTTCGAA301.5936255E-596.74132145
GGCAGCC607.2759576E-1296.74132145
TCACGCC502.2573658E-887.06718145
CCAGACC259.3590136E-487.06718145
AGCGAAA3250.084.700131
ACCGACC353.424754E-582.92113145
AAAGACC353.424754E-582.92113145
TAGCTCC554.3726686E-879.15199145
TATATTC4200.077.615253
CGAAAGC3550.077.5423663
TTATATT4400.074.087282
TCTCGGG502.2963013E-672.555984145
GCCCTCC406.638936E-572.555984145
AGGCTCC809.822543E-1172.555984145
Produced by FastQC (version 0.11.5)