FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9459_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9459_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106750
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACG130930.6214785807523436No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG91190.43284680194612557No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT85370.4052213124480835No Hit
TTCCAACACTGTGTCAAGCTTCCAGGTAGATTGCTTTCTTTGGCATATCC82260.39045923816304734No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC72880.34593568292393495No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG71860.3410941022902575No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG61900.2938174913967011No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT59930.28446659546695147No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA54530.25883469799454134No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG53090.25199952533523196No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT51810.24592381630473475No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA48050.22807642102764922No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC45850.21763379613148215No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC44950.21336181321941378No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA41690.1978877417823662No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC41160.19537201851192598No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC40440.19195443218227126No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT40290.1912424350302599No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC39600.18796724813100746No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA38800.18416992998694673No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG37850.17966061469087458No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT37170.17643289426842293No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT37100.1761006289308176No Hit
TGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACGATCAAGAATCCA36220.17192357897235078No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA34980.1660377358490566No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA33740.16015189272576244No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG32190.1527945888216447No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA31650.1502313990744037No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT31450.14928206953838852No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC31380.14894980420078321No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC30940.1468612792215498No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT30740.1459119496855346No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG28840.13689331909339028No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT28370.1346623946837546No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA28310.13437759582295006No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC27790.13190933902931054No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG27540.13072267710929156No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG27150.12887148451406194No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAAT26650.12649816067402397No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA26590.12621336181321943No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT26240.12455203512519283No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT26120.12398243740358372No Hit
GTGTCAAGCTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT25850.12270084252996322No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT25250.11985285392191763No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA25180.11952058858431232No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT25160.11942565563071081No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC25100.11914085676990625No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA24960.11847632609469562No Hit
GTCGATAGACACATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG24900.11819152723389108No Hit
CTTAAAATGACCATGGTCTCAACACCTGCTTCGAGATACATAACTGACAT24660.11705233179067284No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAAC24530.11643526759226297No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA24340.11553340453304853No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA24310.11539100510264626No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG24290.11529607214904475No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA24130.11453660852023258No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC24050.11415687670582651No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC24020.11401447727542424No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC23930.11358727898421739No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG23880.11334994660021361No Hit
CTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATTTGCTCCATAG23870.11330248012341283No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT23850.11320754716981132No Hit
TCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT23820.11306514773940905No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG23730.1126379494482022No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG23620.11211581820339386No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG23590.11197341877299156No Hit
GCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGT23570.11187848581939006No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA23360.1108816898065741No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC23310.11064435742257031No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGA22940.10888809778094222No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACT22660.10755903643052095No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA22500.10679957280170879No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA22480.10670463984810727No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG22350.1060875756496974No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC22230.1055179779280883No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT22210.10542304497448676No Hit
CTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAATCTCCATGGCCTC22160.10518571259048298No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT22080.10480598077607689No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC21900.10395158419366324No Hit
GACATAATGGATTCCAACACTGTGTCAAGCTTCCAGGTAGATTGCTTTCT21860.10376171828646019No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGCTTCCAGGTAG21770.10333451999525334No Hit
GACTAGCAATCTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATT21730.10314465408805032No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT21720.10309718761124956No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA21600.10252758988964043No Hit
GGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAG21410.101625726830426No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT21290.10105612910881691No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA21250.10086626320161386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC900.0120.90147145
ACCGACC256.448452E-6116.06541145
AGCGTAA800.0108.81131145
GCTGACC1400.098.44833145
TGACGCC554.1472958E-1092.32475145
TGTAGGG259.367201E-487.04905145
AGCGAAA4500.086.935421
CGAAAGC4550.085.9800953
CCTCGCC851.8189894E-1276.80799145
AGACGCC851.8189894E-1276.80799145
CAGTACG2500.075.356559
ACCTACC300.001931716272.54088145
TACTGCC608.004645E-872.54088145
CAAGCTA2650.071.0843354
AAGCTAA2700.069.767965
GAGCGAA1700.068.184651
TAGTGCC651.3933641E-766.96081145
AGGCTCC1550.065.52079145
GCTGCCC451.1908375E-464.48078145
TTCGAGG451.1908375E-464.48078145
Produced by FastQC (version 0.11.5)