FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9424_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9424_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105312
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG102350.486151221291666No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC87250.4144278852730616No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT65600.3115927710477117No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG62850.29853057409068107No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC58450.27763105895943213No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT57340.2723586812785943No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA57080.2711237099299296No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA52340.24860923226581144No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG49380.23454955845024394No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC49140.23340958489763036No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT43580.2070001975954158No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG41620.19769041358240488No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT40800.1937955039443085No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG40780.19370050614825737No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC39180.18610068246416683No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC36630.17398846346764757No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC36090.171423522974267No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG34570.164203690474381No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA33450.15888381389551764No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA32610.1548939064613701No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA32470.1542289218890122No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT31930.15166398139563161No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT31810.15109399461932482No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA31540.14981152437263454No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA30780.14620160812269156No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG30600.14534662795823136No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA30260.14373166542536212No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC29650.1408342326458026No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC29600.1405967381556748No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC29180.13860178443860102No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG28460.13518186378076028No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC27920.13261692328737973No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG27630.13123945524463831No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC26670.126679561034184No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA26640.1265370643401073No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTT26330.12506459850131477No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT26090.12392462494870118No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA25380.12055220318888601No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC25270.12002971531060479No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC25200.11969722302442583No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA25140.11941222963627245No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA25100.11922223404417016No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG25090.1191747351461446No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA25070.11907973735009347No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT24940.11846225167576113No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTTAAC24700.11732227812314754No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT24660.11713228253104528No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT24410.11594481008040612No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT24290.11537482330409934No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG23670.11242989162651426No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA23610.11214489823836087No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC23600.1120973993403353No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG23140.10991245003115929No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT22750.10805999300816221No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA22220.10554255141280722No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA22190.10540005471873053No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT22020.1045925734522959No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC21880.10392758887993798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC202.1227697E-6145.11496145
CGGAGCC458.367351E-11112.867195145
GCTGACC1800.0108.83623145
GCCCGGG352.7498027E-7103.65354145
CAGGGGG650.0100.46421145
CGACTCC1050.096.74331145
GGCCGCC301.593588E-596.7433145
AGGCTCC1100.092.34589145
TGACGCC1050.089.83307145
TGTAGGG259.358686E-487.06898145
GTCTGGG353.4246717E-582.92284145
GTAAGTT554.3726686E-879.15362145
AGCGAAA4950.079.029541
TCACGCC755.4569682E-1177.39465145
GCTGTCC1350.075.2448145
TAGCTCC1300.072.55749145
TTAGGGG1000.072.55748145
AGACGCC809.822543E-1172.55748145
CTTATAA300.001929963172.55748145
TTATATT5150.068.9270552
Produced by FastQC (version 0.11.5)