FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9420_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9420_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623065
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG159100.6065423464534809No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC128920.4914861049954919No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA116610.4445562729097449No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG106470.4058992057002019No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG105660.40281121512429163No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT102660.3913742129912907No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC88780.3384590164559399No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT83340.31771991925476495No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG78440.29903948243753015No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA74260.2831039261322155No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC71650.27315373427650474No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG71150.27124756725433796No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC65790.2508134567767097No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA65530.24982224992518295No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG64350.24532369575286928No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT62120.23682219083400527No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT60890.2321330199594749No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC56650.2159687236115003No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAGTGCATGTGTC56200.21425317329155014No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC50240.1915316623873217No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG49680.18939675532249486No Hit
TAGTAGCACTGCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAA49060.18703310821500801No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC47940.18276329408535436No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA47690.18181021057427094No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT47500.18108586710584756No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC45420.1731562122936336No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT44790.1707544418457034No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT44260.1687339048022066No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC44030.16785706797200983No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA43280.16499781743875963No Hit
ATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA42980.16385411722545953No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG42800.16316789709747948No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA42660.16263417033127275No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC42290.16122360673486932No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC41970.16000365984068257No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG41850.15954617975536253No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG41530.15832623286117575No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA40640.15493325556171883No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA40490.15436140545506877No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA39780.1516546482835919No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA38230.14574553051487477No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA38130.1453642971104414No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG38100.1452499270891114No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC37780.14402998019492463No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA37620.14342000674783126No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC37600.1433437600669446No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTT36940.1408276195976844No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT35850.13667217548936073No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG35760.1363290654253707No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTTAAC35020.1335079382325638No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT33920.12931437078379682No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT33900.12923812410291013No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT33660.12832316393227008No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC33330.12706509369764No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA33300.12695072367630997No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA33240.12672198363364995No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT33130.12630262688877325No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC33000.12580702346300987No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT32730.1247776932710398No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT31910.12165157935468622No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA31370.11959291897074606No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG31260.11917356222586936No Hit
CACTTGGAGGTGTGTTTCATGTATTCAAGTCAGTATTCAATAGCCTGTAT30930.11791549199123925No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA30860.1176486286081359No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT30820.11749613524636256No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA30620.11673366843749583No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT30530.11639055837350581No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAAT30310.11555184488375242No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGC30140.11490374809621569No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT29720.11330256779759555No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG29330.11181575752030545No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA29170.11120578407321208No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAACTTCTGACCTA29020.11063393396656201No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT28590.10899463032749855No Hit
CTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCA28450.10846090356129184No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA28260.10773656009286844No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA28230.10762219007153845No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG28130.10724095666710509No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC28060.10697409328400173No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA28030.10685972326267172No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC28030.10685972326267172No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC28000.10674535324134171No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCATTTGAATCGTTGCA27980.10666910656045504No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT27870.10624974981557835No Hit
TCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT27550.10502980292139158No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT27530.10495355624050491No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT27520.10491543290006157No Hit
CTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGT27400.10445795281474153No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG27030.10304738921833809No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC26980.10285677251612141No Hit
GTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG26950.1027424024947914No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC26840.1023230457499147No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGA26800.10217055238814135No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA26370.10053124874907789No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA26270.10015001534464452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGGG100.00707675145.1011145
GCCCGGG351.893568E-9124.37238145
CAGGGGG600.0120.91759145
GCTGACC2000.0112.45336145
TGACGCC800.099.75701145
AGCGAAA6550.097.336871
GAGCGAA1950.089.168741
TCACGCC1500.087.06067145
CCGAACC259.3624956E-487.06067145
CGACTCC608.258212E-1084.64231145
GGCAGCC353.4264507E-582.91492145
TTCGAGG554.3759428E-879.14606145
GTCTGGG651.5570549E-978.13136145
GTGAGCC651.5570549E-978.13136145
CGAAAGC8200.077.752273
AGCGTAA755.4569682E-1177.38726145
CTCACCC851.8189894E-1276.81823145
GGTGGCC1050.076.00535145
GTACTGA14100.073.481456
TTATATT7250.072.949252
Produced by FastQC (version 0.11.5)