FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9350_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9350_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944188
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCG79610.8431583540566074No Hit
GGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGA74770.7918973763699603No Hit
AGCGAAAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTG53360.5651416878841926No Hit
AGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAG43320.45880693251767657No Hit
ACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCC41540.4399547547734138No Hit
AAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTC39390.4171838659250065No Hit
CCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGT39260.4158070214830097No Hit
GCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGT36220.38361004376247104No Hit
GCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGG36170.3830804882078569No Hit
CATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGACAG36130.38265684376416564No Hit
GTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGC35060.37132435489542337No Hit
CTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGA34800.3685706660114299No Hit
CCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGA34760.3681470215677386No Hit
GTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTG33910.35914457713929854No Hit
GCCGTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGA33670.35660271047715075No Hit
AAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGG31810.33690324384550535No Hit
GTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTG30120.31900426609954796No Hit
GATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCA29760.31519146610632626No Hit
GCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAA27940.2959156439183722No Hit
TTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTG27820.2946447105872983No Hit
AGCGAAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGT27080.28680728837900926No Hit
CTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAG25530.2703910661859715No Hit
CCCCAACCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTGGGTGCATT25370.26869648841120625No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA24680.26138862175753136No Hit
CCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGC24510.2595881328718433No Hit
GGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTG23790.25196253288539994No Hit
AAGCAGCACTGGTCAGAAATCATTAATAACTGCCCCTCATTTGAAGCACT23580.24973839955602062No Hit
TCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTT22750.24094777734942618No Hit
GAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCT22660.23999457735112076No Hit
GCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCGGACCT22210.23522857735959363No Hit
AAGCAGGGCCATGTGGGTAAGAAGGAGTGGAGATTTATGACAAAAAAGAG21220.224743377378234No Hit
GTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGT21130.22379017737992857No Hit
CTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCC20470.21680004405902217No Hit
AAGCAGGGGTGCTTATGTTTGTCGAGGTTTGTAAAAGTGAGTGTAATTTC20210.2140463551750287No Hit
GACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTC19990.21171631073472658No Hit
GCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTAGTGG19940.21118675518011243No Hit
GGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACA19820.20991582184903854No Hit
CACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCT19560.2071621329650451No Hit
GAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCGAGG19320.20462026630289729No Hit
CCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCG19310.20451435519197447No Hit
TTGTAAAAGTGAGTGTAATTTCATTCAAGTCGCAAACATAAGCACCCTTG19240.2037729774155147No Hit
CTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTAGT18910.2002779107550615No Hit
GTCCAATACAAGGCTGACCACTGGGGTAATTACCAAAGCAGCCTCTCTTC18690.19794786631475936No Hit
CCTATAGTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTTGT18310.193923244099692No Hit
GTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAG18080.19148728854846703No Hit
ATCTTATAGCGAAGACTACATGGAAATAGCTGTCTTGATTATTAACAGCG17790.18841586633170512No Hit
GTGCTTATGTTTGTCGAGGTTTGTAAAAGTGAGTGTAATTTCATTCAAGT17790.18841586633170512No Hit
CCCTTGGTGAGCACCCCCAAGTGTAAGGTTTACTCAAAGAGCCTCTCCTC17670.18714493300063123No Hit
GTATTAATTAGCATAATTGCAGTGACCGGGCTGCAATTGCTGCGCGGATG17460.1849207996712519No Hit
GCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTG16760.17750702190665418No Hit
GTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAG16710.17697746635204006No Hit
GTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTA16580.17560062191004333No Hit
ATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCG16200.171575999694976No Hit
GTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGG15630.16553906637237498No Hit
CTGCGGAGGCAGCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCA15610.16532724415052935No Hit
GTGGATATGAGGAGCACAGTGAGGAGCCCATCCAAGGACCACTACAGCCT15480.16395039970853262No Hit
CCGTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAG15470.1638444885976098No Hit
GGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGC15410.16320902193207285No Hit
GGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCCCTGTC15280.1618321774900761No Hit
TGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCA15170.16066715526992506No Hit
GTGTAAGGTTTACTCAAAGAGCCTCTCCTCTGAAGTGGAGGCCTGCTGTG15140.1603494219371566No Hit
CTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGA14910.15791346638593162No Hit
CTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATA14620.1548420441691697No Hit
GTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCAC14550.15410066639270992No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT14530.15388884417086426No Hit
GGTCAGAAATCATTAATAACTGCCCCTCATTTGAAGCACTCAGGGAGTGT14450.15304155528348168No Hit
GAAATGGATTCATGCCTGGAACAGCAGATGAGAAGAAACTCAAGTCGATA14320.15166471084148497No Hit
GAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCG14300.15145288861963932No Hit
CACTAAGGCCTACTGTAGCTAGACGCACTCAGCAGGATGCAGTGTTACAG14230.15071151084317952No Hit
GATTGAGGCTCAATCTCTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCG14170.1500760441776426No Hit
GCGAAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTC14100.14933466640118281No Hit
GGGTAAGAAGGAGTGGAGATTTATGACAAAAAAGAGATATTACATGCCTC13850.1466868886281122No Hit
GGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGC13690.14499231085334702No Hit
GGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAA13520.14319182196765898No Hit
TCTTAACCCCCCTCCGATCAGAAATGGATTCATGCCTGGAACAGCAGATG13510.14308591085673616No Hit
CCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGACG13360.14149724419289378No Hit
CATAAGCACCCTTGGTGAGCACCCCCAAGTGTAAGGTTTACTCAAAGAGC13280.1406499553055112No Hit
GCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTT13220.14001448863997423No Hit
GATTAAAGTGGGAGGTGTTGCTTTGTGGAACAGAACACATCTTTTTTTCT13170.1394849330853601No Hit
GCCTTGCGTTTCCGTGGAGACAGGGGTTGGGGCGCTGGGATGCGGCAGCC13100.13874355530890034No Hit
GCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCGAGGTCGAG13080.1385317330870547No Hit
TCCCAGCGCCCCAACCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTG12940.13704897753413517No Hit
GTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGCT12940.13704897753413517No Hit
GGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCT12820.13577804420306125No Hit
GTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCG12770.13524848864844713No Hit
CCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTG12730.13482484420475582No Hit
TCTTTATTCAGTGCATTTTTTTATTTCCATTTCTCTCTGCTGCTCTTAGA12520.1326007108753765No Hit
GTGTGAGGGAGAGAGATTGAGTGAGAGAGAGAAAGTTTTGTTCCTCTTAA12470.13207115532076238No Hit
GTAAAAGTGAGTGTAATTTCATTCAAGTCGCAAACATAAGCACCCTTGGT12370.13101204421153415No Hit
ATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAG12350.1308002219896885No Hit
GAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGC12340.13069431087876568No Hit
ATCTAAGACAGCGGAAGGGCACGGGAGGTTGGCCAAAAGTGTTTTGGTGT12320.13048248865692003No Hit
GCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTC12290.13016475532415156No Hit
GCGAAAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGC12240.12963519976953744No Hit
TCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGA12230.1295292886586146No Hit
CCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCG12110.1282583553275407No Hit
GCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGA12060.12772879977292656No Hit
CCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTG12020.12730515532923528No Hit
GCGTTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGC12000.12709333310738963No Hit
GTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATC11910.1261401331090842No Hit
CTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCCCTGTCTCC11810.12508102199985596No Hit
GCAGGATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTG11550.12232733311586252No Hit
GACGAGAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCC11230.11893817756633213No Hit
CTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAG11180.118408622011718No Hit
GACCTATAGTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTT11130.11787906645710389No Hit
GATATGAGGAGCACAGTGAGGAGCCCATCCAAGGACCACTACAGCCTGAC11120.11777315534618107No Hit
GGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCC11070.11724359979156693No Hit
CTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAAT11020.11671404423695281No Hit
GGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGG11020.11671404423695281No Hit
GGATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGT10960.11607857757141586No Hit
CTCAGGGAGTGTGGTGTGGCATCTGTGTTTGAGAACAATTACAACCCTGT10660.11290124424373112No Hit
ACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGACAGGGGTTGG10640.11268942202188546No Hit
CCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTGGGTGCATTGTGGAT10510.11131257757988876No Hit
TTCTTAACCCCCCTCCGATCAGAAATGGATTCATGCCTGGAACAGCAGAT10510.11131257757988876No Hit
CAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGACAGGGGTTGGG10430.11046528869250616No Hit
GTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTG10320.10930026647235508No Hit
GGGTTGGGGCGCTGGGATGCGGCAGCCGTGGCGACCGTGGAGACCTCCTC10270.10877071091774096No Hit
CACAAGTACAGTACATTCAGGAGGCGTTGCTAGGAAACGCTACTGGCTGG10200.10802933314128119No Hit
GGCTCAATCTCTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCGATCAGA10140.10739386647574424No Hit
GAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGT10120.1071820442538986No Hit
CACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCG10100.10697022203205295No Hit
GTACAGTACATTCAGGAGGCGTTGCTAGGAAACGCTACTGGCTGGTGAGT10060.10654657758836165No Hit
AGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGCTGCCG10010.10601702203374751No Hit
GAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCC9980.10569928870097904No Hit
GATTATTAACAGCGCCCATGAGACGCACAAGTACAGTACATTCAGGAGGC9940.10527564425728773No Hit
AGCGAAAGCAGGGCCATGTGGGTAAGAAGGAGTGGAGATTTATGACAAAA9930.10516973314636492No Hit
AGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCAC9910.10495791092451927No Hit
CTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCGGACCTCTC9880.10464017759175079No Hit
GTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTTGTGGAACA9570.10135693315314323No Hit
ACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTC9530.10093328870945192No Hit
CCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACAC9470.10029782204391499No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC100.0070800683145.07347145
ATGGGCC7400.0120.56782145
TACGGCC2750.0110.78338145
ATAGGAG553.6379788E-12105.401823
GAGCGAA3100.0102.8517761
GGTTCTA800.099.637661
GATAGGA607.2759576E-1296.618332
GCGAAAG23750.094.279162
GTTCTAT850.093.776612
CACTGGT3800.093.445057
GCGCACC554.1472958E-1092.31948145
ACTGGTC3950.091.7359858
CGCCACC2650.090.328766145
CGAAAGC25550.087.353563
TCTGGAC259.4052503E-486.9565053
GTCGCCC4900.085.85981145
TTCTATC950.083.9053963
AGCGAAA26950.083.0845951
GAAAGCA28450.078.453484
GCACTGG4600.077.193746
Produced by FastQC (version 0.11.5)