FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9350_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9350_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947400
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCG79870.8430441207515306No Hit
GGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGA74920.7907958623601435No Hit
AGCGAAAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTG53550.5652311589613679No Hit
AGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAG43410.45820139328689047No Hit
ACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCC41610.43920202659911334No Hit
AAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTC39530.4172472028710154No Hit
CCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGT39370.4155583702765463No Hit
GCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGT36320.3833649989444796No Hit
GCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGG36240.3825205826472451No Hit
CATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGACAG36190.3819928224614735No Hit
GTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGC35100.37048765041165294No Hit
CTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGA34880.368165505594258No Hit
CCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGA34830.36763774540848637No Hit
GTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTG33980.3586658222503694No Hit
GCCGTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGA33770.3564492294701288No Hit
AAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGG31870.3363943424108085No Hit
GTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTG30160.3183449440574203No Hit
GATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCA29830.31486172683132785No Hit
GCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAA27980.29533459995777916No Hit
TTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTG27850.293962423474773No Hit
AGCGAAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGT27100.2860460206881993No Hit
CTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAG25600.27021321511505175No Hit
CCCCAACCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTGGGTGCATT25430.26841883048342835No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA24730.26103018788262616No Hit
CCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGC24560.25923580325100276No Hit
GGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTG23880.25205826472450915No Hit
AAGCAGCACTGGTCAGAAATCATTAATAACTGCCCCTCATTTGAAGCACT23680.24994722398142283No Hit
TCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTT22800.24065864471184295No Hit
GAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCT22670.23928646822883684No Hit
GCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCGGACCT22250.23485328266835548No Hit
AAGCAGGGCCATGTGGGTAAGAAGGAGTGGAGATTTATGACAAAAAAGAG21310.2249313911758497No Hit
GTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGT21210.22387587080430651No Hit
CTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCC20550.2169094363521216No Hit
AAGCAGGGGTGCTTATGTTTGTCGAGGTTTGTAAAAGTGAGTGTAATTTC20290.21416508338610937No Hit
GACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTC20020.21131517838294278No Hit
GCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTAGTGG19980.21089297023432554No Hit
GGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACA19890.20994300189993664No Hit
CACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCT19640.20730420097107874No Hit
GAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCGAGG19380.2045598480050665No Hit
CCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCG19350.20424319189360352No Hit
TTGTAAAAGTGAGTGTAATTTCATTCAAGTCGCAAACATAAGCACCCTTG19320.2039265357821406No Hit
CTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTAGT18950.20002111040743087No Hit
GTCCAATACAAGGCTGACCACTGGGGTAATTACCAAAGCAGCCTCTCTTC18740.1978045176271902No Hit
CCTATAGTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTTGT18370.19389909225248048No Hit
GTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAG18110.19115473928646823No Hit
GTGCTTATGTTTGTCGAGGTTTGTAAAAGTGAGTGTAATTTCATTCAAGT17810.18798817817183872No Hit
ATCTTATAGCGAAGACTACATGGAAATAGCTGTCTTGATTATTAACAGCG17800.1878826261346844No Hit
CCCTTGGTGAGCACCCCCAAGTGTAAGGTTTACTCAAAGAGCCTCTCCTC17680.1866160016888326No Hit
GTATTAATTAGCATAATTGCAGTGACCGGGCTGCAATTGCTGCGCGGATG17510.1848216170572092No Hit
GCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTG16810.17743297445640702No Hit
GTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAG16750.1767996622334811No Hit
GTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTA16640.17563858982478361No Hit
ATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCG16230.17131095630145662No Hit
GTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGG15660.16529449018366055No Hit
CTGCGGAGGCAGCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCA15620.16487228203504328No Hit
GTGGATATGAGGAGCACAGTGAGGAGCCCATCCAAGGACCACTACAGCCT15550.16413341777496304No Hit
CCGTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAG15540.16402786573780873No Hit
GGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGC15440.16297234536626556No Hit
GGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCCCTGTC15340.1619168249947224No Hit
TGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCA15210.16054464851171626No Hit
GTGTAAGGTTTACTCAAAGAGCCTCTCCTCTGAAGTGGAGGCCTGCTGTG15150.15991133628879037No Hit
CTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGA14930.15758919147139538No Hit
CTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATA14650.1546337344310745No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT14590.15400042220814864No Hit
GTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCAC14570.15378931813383998No Hit
GGTCAGAAATCATTAATAACTGCCCCTCATTTGAAGCACTCAGGGAGTGT14470.1527337977622968No Hit
GAAATGGATTCATGCCTGGAACAGCAGATGAGAAGAAACTCAAGTCGATA14350.15146717331644502No Hit
GAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCG14310.15104496516782773No Hit
CACTAAGGCCTACTGTAGCTAGACGCACTCAGCAGGATGCAGTGTTACAG14260.15051720498205615No Hit
GATTGAGGCTCAATCTCTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCG14200.14988389275913025No Hit
GCGAAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTC14140.14925058053620435No Hit
GGGTAAGAAGGAGTGGAGATTTATGACAAAAAAGAGATATTACATGCCTC13880.1465062275701921No Hit
GGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGC13700.1446062909014144No Hit
GGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAA13540.14291745830694533No Hit
TCTTAACCCCCCTCCGATCAGAAATGGATTCATGCCTGGAACAGCAGATG13510.14260080219548238No Hit
CCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGACG13360.1410175216381676No Hit
CATAAGCACCCTTGGTGAGCACCCCCAAGTGTAAGGTTTACTCAAAGAGC13300.1403842094152417No Hit
GCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTT13260.13996200126662445No Hit
GATTAAAGTGGGAGGTGTTGCTTTGTGGAACAGAACACATCTTTTTTTCT13250.13985644922947013No Hit
GCCTTGCGTTTCCGTGGAGACAGGGGTTGGGGCGCTGGGATGCGGCAGCC13130.1385898247836183No Hit
GCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCGAGGTCGAG13110.13837872070930968No Hit
TCCCAGCGCCCCAACCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTG12970.13690099218914925No Hit
GTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGCT12940.1365843360776863No Hit
GGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCT12880.1359510238547604No Hit
GTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCG12800.13510660755752588No Hit
CCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTG12740.13447329533459995No Hit
TCTTTATTCAGTGCATTTTTTTATTTCCATTTCTCTCTGCTGCTCTTAGA12550.13246780662866794No Hit
GTGTGAGGGAGAGAGATTGAGTGAGAGAGAGAAAGTTTTGTTCCTCTTAA12490.13183449440574202No Hit
GTAAAAGTGAGTGTAATTTCATTCAAGTCGCAAACATAAGCACCCTTGGT12400.13088452607135317No Hit
ATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAG12390.13077897403419886No Hit
GAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGC12350.1303567658855816No Hit
ATCTAAGACAGCGGAAGGGCACGGGAGGTTGGCCAAAAGTGTTTTGGTGT12340.13025121384842728No Hit
GCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTC12290.1297234536626557No Hit
TCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGA12260.12940679755119275No Hit
GCGAAAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGC12260.12940679755119275No Hit
CCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCG12160.1283512771796496No Hit
GCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGA12080.12750686088241503No Hit
CCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTG12050.12719020477095208No Hit
GCGTTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGC12030.12697910069664345No Hit
GTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATC11930.12592358032510026No Hit
CTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCCCTGTCTCC11860.12518471606502005No Hit
GCAGGATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTG11570.12212370698754485No Hit
GACGAGAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCC11250.11874604179860672No Hit
CTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAG11200.11821828161283512No Hit
GACCTATAGTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTT11150.11769052142706356No Hit
GATATGAGGAGCACAGTGAGGAGCCCATCCAAGGACCACTACAGCCTGAC11140.11758496938990923No Hit
GGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCC11110.11726831327844628No Hit
CTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAAT11050.11663500105552037No Hit
GGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGG11040.11652944901836605No Hit
GGATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGT10990.11600168883259447No Hit
CTCAGGGAGTGTGGTGTGGCATCTGTGTTTGAGAACAATTACAACCCTGT10680.11272957568081064No Hit
ACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGACAGGGGTTGG10660.112518471606502No Hit
CCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTGGGTGCATTGTGGAT10540.1112518471606502No Hit
TTCTTAACCCCCCTCCGATCAGAAATGGATTCATGCCTGGAACAGCAGAT10530.11114629512349587No Hit
CAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGACAGGGGTTGGG10490.11072408697487862No Hit
GTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTG10330.10903525438040955No Hit
GGGTTGGGGCGCTGGGATGCGGCAGCCGTGGCGACCGTGGAGACCTCCTC10280.10850749419463795No Hit
CACAAGTACAGTACATTCAGGAGGCGTTGCTAGGAAACGCTACTGGCTGG10210.10776862993455774No Hit
GGCTCAATCTCTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCGATCAGA10180.10745197382309478No Hit
GAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGT10150.10713531771163183No Hit
CACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCG10120.10681866160016887No Hit
GTACAGTACATTCAGGAGGCGTTGCTAGGAAACGCTACTGGCTGGTGAGT10120.10681866160016887No Hit
AGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGCTGCCG10030.10586869326578002No Hit
GAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCC10010.10565758919147139No Hit
AGCGAAAGCAGGGCCATGTGGGTAAGAAGGAGTGGAGATTTATGACAAAA9970.10523538104285414No Hit
GATTATTAACAGCGCCCATGAGACGCACAAGTACAGTACATTCAGGAGGC9960.10512982900569981No Hit
AGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCAC9920.10470762085708255No Hit
CTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCGGACCTCTC9900.1044965167827739No Hit
GTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTTGTGGAACA9600.1013299556681444No Hit
ACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTC9540.10069664344521849No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG100.007081796145.06166145
ATGGGCC7550.0123.92684145
TACGGCC2100.0120.88472145
GGTCCGG203.8594598E-4108.79624145
ACGATTT203.8594598E-4108.79624145
AATTGCC404.800313E-9108.79624145
GAGCGAA3300.0107.577931
TAGACTT301.6048558E-596.6005864
GTAGACT301.6048558E-596.6005863
GCGAAAG25150.094.488052
TGGTCCC1750.091.18162145
ACTGGTC4500.090.179588
CGAAAGC26500.089.674513
CACTGGT4550.089.188617
CTACGGG259.412125E-486.940544
CGCCACC3100.086.56905145
AGCGAAA28550.083.743071
TCAGGGG703.092282E-1182.89237145
CTAATAG353.4488603E-582.800512
GTAGAAA451.2378605E-680.500491
Produced by FastQC (version 0.11.5)