FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9332_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9332_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706562
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG72290.42360019735585347No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG52230.30605392596342823No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT46150.27042674101497627No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG43020.2520857724477634No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC39460.23122511810294613No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC37330.2187438839022549No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG36160.21188799469342456No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA34950.2047977161099333No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA31890.18686692894837692No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG29790.17456148677868136No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC29250.17139723022075964No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT28950.16963930991080312No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA28450.1667094427275423No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT27990.1640139649189423No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG27720.16243183663998143No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG27430.16073251367369015No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA25680.15047797853227718No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC25480.14930603165897285No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA24740.1449698282277468No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC24730.14491123088408156No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA24650.1444424521347598No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC23820.1395788726105468No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA23720.13899289917389465No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG23560.13805534167525116No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCCCCTTGC22640.13266438605805123No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG22470.13166823121574253No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT21030.12323021372795129No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT20780.12176528013632087No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC20550.12041754123202086No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA20510.12018315185736No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT20140.11801505014174696No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT19860.1163743245191209No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT19510.11432341749083831No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA19380.11356165202319048No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC19190.11244830249355137No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT18600.10899105921730355No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA18280.10711594422001662No Hit
ATATGGGATCAAGCACTCTCGAACTGAGAAGCGGGTACTGGGCCATAAGG18230.10682295750169053No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT18220.10676436015802532No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG18120.10617838672137316No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA18060.10582680265938185No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC18030.1056510106283862No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT17750.10401028500576011No Hit
GTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG17630.1033071168817775No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA17480.10242815672679927No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC17400.10195937797747752No Hit
TGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA17340.10160779391548622No Hit
CTATAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT17230.10096322313516884No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC17110.10026005501118623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAA100.0070763193145.10258145
ACCGACC501.8189894E-12116.08207145
CTCGGCC256.4434953E-6116.08207145
GCTGACC1650.0114.32325145
AGCGTAA451.0868462E-896.735054145
CGGAGCC301.5941758E-596.735054145
GTGAGCC406.0936145E-790.68911145
TGACGCC502.2584572E-887.061554145
AGACGCC608.258212E-1084.64317145
TAAACTA5150.081.562215
GAAGCCC900.080.61255145
CGAAAGC4000.079.663783
AGCGAAA4050.078.677971
TACTGAT3150.073.5862667
GGCCGCC406.641222E-572.55129145
CGACTAA406.641222E-572.55129145
GCCCGGG406.641222E-572.55129145
AAACTAT5850.071.8131566
TTTAAAC6100.068.85993
GCAATCC1150.063.088078145
Produced by FastQC (version 0.11.5)