FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9320_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9320_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596558
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT82810.5186783067073041No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA59390.3719877386227121No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA57810.3620914492301564No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT50100.31380006238420405No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT38000.23801202336526453No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC36920.23124747112225177No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG36480.22849154243065392No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT35040.21947213943997024No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC30880.19341608635577287No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA29980.18777895948659554No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA29190.18283081479031768No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT29100.18226710210339994No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT28470.17832111329497582No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT28430.17807057432301238No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC28270.17706841843515864No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA28080.17587835831833232No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG27200.17036650093513672No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27040.16936434504728298No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT26850.16817428493045666No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA26050.16316350549118794No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT25710.16103392422949872No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA25540.1599691335986541No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA24770.15514625838835794No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT24710.1547704499304128No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG24600.15408146775751336No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA24320.1523276949537693No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC22950.14374673516402162No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT22780.14268194453317698No Hit
GTAGAAAGATAATCAGAAGATCAATAGTCGCATCAAACCCATTGGAACTA22420.14042709378550608No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT22290.1396128421266249No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC21560.1350405058882922No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG21350.13372517628548414No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG20820.1304055349069686No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC20810.13034290016397776No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT20550.1287143968462154No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC20320.12727379775742567No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA19780.12389152163591927No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCA19220.12038397602843116No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG19140.11988289808450428No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG18820.1178785863087968No Hit
GTTAGATACAAATCCATCTCTATAGAACATGCTTGTAAATTCAAACATTC18330.11480948390224471No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA18210.1140578669863544No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA18150.11368205852840925No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG17910.11217882469662863No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG17780.11136457303774745No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC17540.10986133920596684No Hit
GATATATCCCTCAGTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC17460.10936026126203996No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG17240.10798229691624107No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA17100.10710541051436903No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA17060.10685487154240561No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA17010.1065416978274513No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT17010.1065416978274513No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATAT17000.10647906308446045No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCAGCAGG16810.10528900296763412No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG16750.10491319450968897No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG16580.10384840387884436No Hit
CTTATGGGATGGACTGCAATCTTCTGATGATTTTGCTTTGTTTGTTAATG16470.10315942170594493No Hit
GATCTATACTAAATACTGATCAGAGAAACATGATTCTTGAAGAACAATGC16430.10290888273398148No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT16200.10146828364519171No Hit
GGATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTATAAGACCAAG16150.10115510993023744No Hit
GTATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC16120.10096720570126484No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTT15970.10002768455640197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC450.0128.99425145
GGCACCC700.0124.38731145
CGCACCC256.43985E-6116.09482145
CTAGGCC256.43985E-6116.09482145
AGCACGC3050.0109.245141
ATATGCC203.8539365E-4108.83889145
AACGGCC203.8539365E-4108.83889145
GGACGGG203.8539365E-4108.83889145
CGGAGCG203.880458E-4108.651412
GCACGCA3150.0105.777042
CAGCGCC352.7490933E-7103.65609145
TGACGCC700.0103.65609145
ATAAGCC352.7490933E-7103.65609145
CGTTACC301.5932748E-596.74568145
CACGCAC3450.096.582063
AAACACG3350.090.818825
AGCCACC406.089449E-790.69907145
AACACGT800.090.551356
GAGCAGA1050.089.6805341
GCTGCCC259.357356E-487.07111145
Produced by FastQC (version 0.11.5)