FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9320_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9320_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601765
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT82880.517429210901724No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA59600.3720895387275911No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA57900.3614762465155625No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT50230.31359156930011584No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT38120.23798747007207677No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC37030.23118247683024665No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG36550.22818578255861502No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT35110.2191956997437202No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC30920.19303705599760265No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA30010.18735582310763438No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA29270.182735919438869No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT29130.18186188360964312No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT28530.1781160157701036No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT28520.1780535846394446No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC28290.17661766863428782No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA28120.17555633941308493No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG27230.16999996878443469No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27080.1690635018245498No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT26870.16775244808071096No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA26070.1627579576279916No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT25820.1611971793615168No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA25570.15963640109504204No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT24790.15476677290364066No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA24780.15470434177298167No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG24640.1538303059437558No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA24370.15214466541596303No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC22990.14352916938502214No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT22820.1424678401638193No Hit
GTAGAAAGATAATCAGAAGATCAATAGTCGCATCAAACCCATTGGAACTA22470.1402827505907546No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT22330.1394087147615287No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC21630.13503853561539925No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG21370.13341532621826546No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC20880.13035620081597488No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG20860.13023133855465688No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT20610.1286705602881821No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC20360.1271097820217073No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA19800.12361363870480375No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCA19240.12011749538790022No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG19170.11968047747328728No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG18850.11768268129219955No Hit
GTTAGATACAAATCCATCTCTATAGAACATGCTTGTAAATTCAAACATTC18370.11468598702056793No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA18290.11418653797529599No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA18160.1133749332767291No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG17960.11212631066354926No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG17800.1111274125730054No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC17590.10981635882916657No Hit
GATATATCCCTCAGTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC17520.10937934091455363No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG17300.1080058560400558No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA17140.10700695794951195No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA17080.10663237116555799No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA17060.10650750890424002No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT17050.10644507777358102No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATAT17020.10625778438160403No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCAGCAGG16860.10525888629106019No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG16790.10482186837644725No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG16610.10369810802458537No Hit
CTTATGGGATGGACTGCAATCTTCTGATGATTTTGCTTTGTTTGTTAATG16510.10307379671799548No Hit
GATCTATACTAAATACTGATCAGAGAAACATGATTCTTGAAGAACAATGC16480.10288650332601848No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT16240.10138815619020268No Hit
GTATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC16160.10088870714493074No Hit
GGATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTATAAGACCAAG16160.10088870714493074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCC151.2264763E-4145.10867145
CCCGCCC100.0070753912145.10867145
CAGTGCG100.0071071843144.891245
GGCACCC301.0971053E-7120.9239145
CTAGGCC301.0971053E-7120.9239145
AGCACGC3200.0113.189211
GCACGCA3300.0109.759232
CCTCACC203.8549796E-4108.831505145
CGCACCC203.8549796E-4108.831505145
CACGCAC3350.0108.121053
ATAAGCC501.9463187E-10101.57607145
AAACACG3750.0100.457935
GAAACAC3800.099.129914
AACACGA3000.096.600196
ACAACGC2850.088.9738548
CAACGCA2850.088.9738549
GAGCTCC259.3598873E-487.0652145
AGCCACC259.3598873E-487.0652145
ACACGTG1000.086.940177
AACACGT1000.086.940176
Produced by FastQC (version 0.11.5)