FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9313_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9313_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383716
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG97990.41108085023551466No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC90090.37793931827449245No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG70430.29546305012845486No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT66030.27700447536535394No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT61650.25862980321481255No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA59740.2506171037153755No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC59720.25053320110281596No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA58720.24633807047483844No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG56090.23530487692325763No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC55200.23157121066435765No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG48940.20530969293321855No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC48860.20497408248298035No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG45320.19012332005994No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT43360.18190086402910413No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA41490.1740559697547862No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC40810.17120328092776152No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG39730.16667253984954583No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG39460.1655398545799919No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT37940.1591632560254661No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT37790.1585339864312695No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA37250.15626861589216165No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG36860.15463251494725044No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC36780.15429690449701222No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA36580.15345787837141672No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC36420.15278665747094033No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA35050.14703932851061116No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT33880.1421310256758775No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC32510.13638369671554834No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC31710.13302759221316632No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC30760.1290422181165877No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT29980.12577001622676526No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA29430.12346269438137765No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC28850.1210295186171507No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTT28760.12065195686063272No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA28700.12040024902295408No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA28590.11993878465387656No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA28470.11943536897851925No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG28280.11863829415920354No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT28100.11788317064616759No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT27820.11670853407033388No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT27790.11658268015149456No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTTAAC27310.11456901745006536No Hit
ATATGGGATCAAGCACTCTCGAACTGAGAAGCGGGTACTGGGCCATAAGG26760.11226169560467775No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA26640.11175827992932043No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT26320.11041583812836764No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA25440.10672412317574746No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC25250.10592704835643173No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT25180.10563338921247331No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC25180.10563338921247331No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAACTTCTGACCTA25150.10550753529363398No Hit
TGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA25120.10538168137479466No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA24910.10450070394291938No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC24830.10416509349268119No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA24830.10416509349268119No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC24780.10395533696128229No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT24630.10332606736708567No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT24580.1031163108356868No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT24520.10286460299800816No Hit
GTATTGGCTTCTAGTGGCCTTGGGGACTAAAGATTGAAATGGTTCAAATT24480.10269679777288906No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA24370.10223533340381152No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT24190.1014802098907756No Hit
TTCTTAAACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT24110.10114459944053737No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG24060.1009348429091385No Hit
GTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG23890.10022167070238233No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA23870.10013776808982279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC403.274181E-11126.95656145
GCTGACC2500.0124.78016145
TGACGCC700.0114.00181145
AGCGAAA4850.0113.509591
CAGGGGG650.0100.44915145
GAAGCCC900.096.72881145
GGTGGCC451.0873919E-896.72881145
TCACGCC750.096.7288145
CTCGGCC301.5948182E-596.7288145
CGAAAGC5750.095.7428743
GTCCGGG259.3644165E-487.05592145
GCAATCC1250.087.05592145
GCTGTCC502.2599124E-887.05592145
CTAGTCT259.4137475E-486.940869
TACTAGT259.415317E-486.937227
ATCGACC950.084.001335145
GAGCGAA950.083.874471
CTCACCC1350.080.60734145
GTACCCC451.2287073E-680.60734145
TTAGGGG651.5570549E-978.12711145
Produced by FastQC (version 0.11.5)