FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9105_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9105_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCATAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT80.40691759918616477No Hit
GATATACAGAAAGAACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA50.254323499491353No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC50.254323499491353No Hit
GTGCTATACTACAAAAATCCCGGAACCAAATTGGGCTGTCTCTGGTTATT50.254323499491353No Hit
GCTTTAGCACTTCAATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA50.254323499491353No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT40.20345879959308238No Hit
CTATTGTCCAAGCAATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT40.20345879959308238No Hit
CATTATAGGTATTACTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT30.1525940996948118No Hit
AGCACGCACTTTCGTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT30.1525940996948118No Hit
GTTTAATATGCTAACTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA30.1525940996948118No Hit
GCTATGAACACAGAAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT30.1525940996948118No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT30.1525940996948118No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC30.1525940996948118No Hit
ATATAGTAGCGCTTAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT30.1525940996948118No Hit
ATATAGTAGCGCTAAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT30.1525940996948118No Hit
GATTACAATTTGCATAAATGCATAGTAAGAAATACAGTAAAATTGAATTT30.1525940996948118No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT30.1525940996948118No Hit
CCAAAGATGTGGGAGATGGGGACAACCAAAGAACTGGTGCAAAACACCTA30.1525940996948118No Hit
ATATTGTCTCATGATCATGTACCTGAATCCTGGAAATTATTCAATGCAAG30.1525940996948118No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC30.1525940996948118No Hit
GTAGTAACAAGAGAATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC30.1525940996948118No Hit
CTACAATTCTACCGCTTTGTGGTAGTCCTCCGTCTTCTGTTTGATCTGGG30.1525940996948118No Hit
GTTTATTGTCTCTATATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC30.1525940996948118No Hit
TTCATGAAGCATTAGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA30.1525940996948118No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG30.1525940996948118No Hit
CAATACTTCCATGATGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT30.1525940996948118No Hit
ATACTTCCATGTTATCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCTGA30.1525940996948118No Hit
GTAAGAAATACAGAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA30.1525940996948118No Hit
CTATAGAACATGCATGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT30.1525940996948118No Hit
CTTTACATACAGTACCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG30.1525940996948118No Hit
TGCTAAAGCAGAGATCTATGGGAAATTCAGCTCTTGTGAAGAAATACCTA20.10172939979654119No Hit
TCTCAATACTCCAGTGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT20.10172939979654119No Hit
CTGCTTCACCAATAAAAGGCAATGACCCTTTTATTACTTTGCTCCTGCCA20.10172939979654119No Hit
ACATTGGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGA20.10172939979654119No Hit
AGCAGGCACTTTCGTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20.10172939979654119No Hit
GCCATAAATAAGATAACAAAAAATCTCAATTCTTTGAGTGAACTAGAAGT20.10172939979654119No Hit
GCCTTCTGCTATTACAAATGCTTCATGAAAGCTCACACATTTTCTCATTT20.10172939979654119No Hit
GCTCTGAGATCATGCACTTTTTCATCCAGCTCGAGTATTTCGTTGTGGAG20.10172939979654119No Hit
CCCAAAGACCAGGAAAGAAAAAGAAGATTCATCACAGAGCCCTTATCGGG20.10172939979654119No Hit
AAATAGAGTTTTCTACTGTTGGGATTTTGCCCAATTTGACTGAAATTAGA20.10172939979654119No Hit
TTCATGGCCTTCTACTATTTCAAATGCTTCATGAAAGCTCACACATTTTC20.10172939979654119No Hit
GACAGAAGATGGAGAAGGCAAAGCAGAACTAGCAGAAAAATTACACTGTT20.10172939979654119No Hit
ATCTGGAATGGTTGAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT20.10172939979654119No Hit
GCTTATGTGTACCGAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC20.10172939979654119No Hit
CATTATAGGTATTGCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT20.10172939979654119No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT20.10172939979654119No Hit
ATTGAGTACTATGACTTTTATTCATCCATTATTTCATCTACTGATTCTAA20.10172939979654119No Hit
GGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAG20.10172939979654119No Hit
TCTTTTTAGTTTGACCCTTTCAGCATCTTTTGTCATTGTGTTTAATGACA20.10172939979654119No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA20.10172939979654119No Hit
GTCTCATGGTCATATACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA20.10172939979654119No Hit
GAACACATGAGTAGTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA20.10172939979654119No Hit
GTTGTTGCCCTTTTGTTTCTCACCACCAAAGTTAAGGAGCAATGGGGAGC20.10172939979654119No Hit
CAATTACTATGTGATCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC20.10172939979654119No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC20.10172939979654119No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT20.10172939979654119No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT20.10172939979654119No Hit
GTTCCATATAGTGCCTTTCTTTTAATGTCTGATGAACTGGGGCTGGCCAA20.10172939979654119No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG20.10172939979654119No Hit
GAATACTTATGGGATGGACTGCAATCTTCCGATGATTTTGCTTTGTTTGT20.10172939979654119No Hit
GATCTCAATACTCAAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGAC20.10172939979654119No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA20.10172939979654119No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAAGCCAAAAAC20.10172939979654119No Hit
CCTAATTAGCCCCGATAGATTCGGAGAAACCAAAGGAAACTCAGCTCCCT20.10172939979654119No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC20.10172939979654119No Hit
ATCTGAAGGAGGTGATTGTTGTGTTTAGTGCCGTTGCAGCAGGCTGGTTT20.10172939979654119No Hit
GTATAGTATGGTTCTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG20.10172939979654119No Hit
TATCAGCTCTCCATTTCATGGCTTGGACAATAGGACATTTAAATCAAATA20.10172939979654119No Hit
GGCGACATCACCTACGTCTATAGCTGTCAGACATGATTTTAAAGGTTCAG20.10172939979654119No Hit
GGAACGCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGC20.10172939979654119No Hit
AAGTAATACCAATAACCAATAGAATAGTAAATGAAAAAGGAGAAAGTTTC20.10172939979654119No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA20.10172939979654119No Hit
GAATGGGGAAGGAATTGCAAAGGATGTAATGGAAGTGCTAAAGCAGAGCT20.10172939979654119No Hit
CATTAGTTCCATGATATTTCCCAGCTTCTCTTTCTTTTTCCAAAAGTAAT20.10172939979654119No Hit
CTTTTTCCAGTTTATTTGCTGACATTGATTACAATTTGCTTAAATGCATA20.10172939979654119No Hit
GTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGATTCATGATAAC20.10172939979654119No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT20.10172939979654119No Hit
GACAAAAGATGCTAAAAGGGGCAAACTAAAAAGAAGAGCGATTGCAACCG20.10172939979654119No Hit
CTCCAATGTTGCTGTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT20.10172939979654119No Hit
CTTCAATAGACAAATGTCCTACAAAGGGATTTTGAGGGTGAAGTAACCGC20.10172939979654119No Hit
CTTCCATTACATCGTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA20.10172939979654119No Hit
CTATTTACTCTGTCCCCCTTGAAACAAGCTTTGGTCATGTCGTATCCTTG20.10172939979654119No Hit
CTGTGAGGCTGCTAGAAACAGACCTGGATGTTCCTCATCCATTCTATCCA20.10172939979654119No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT20.10172939979654119No Hit
TCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCTGAATTTC20.10172939979654119No Hit
GCCTCTATCTGCTATTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATT20.10172939979654119No Hit
AGTTAGGACTTCTGTTTGTGGATTGTAAGAGAATAGAGGAGACCCTTTCC20.10172939979654119No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC20.10172939979654119No Hit
ATATAAATCCGTATTTTCTATTCATAGATGTACCTATACAGGCAGCAATT20.10172939979654119No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT20.10172939979654119No Hit
CAACTGTAGATAAATTAACCCAGGGGAGACAGACTTTCGATTGGACAGTA20.10172939979654119No Hit
GTACTTCCTTCATGGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT20.10172939979654119No Hit
GCTATCCTGTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCA20.10172939979654119No Hit
TTGCAATTCCTTCGCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT20.10172939979654119No Hit
GTATTTACACAGTATCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT20.10172939979654119No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT20.10172939979654119No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT20.10172939979654119No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC20.10172939979654119No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC20.10172939979654119No Hit
TAGTAAGAAATACGGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAA20.10172939979654119No Hit
GTATTCAGGGTCCGTTAGGTTGTACTTCAATACTATTTCTGGGATATGTA20.10172939979654119No Hit
GCTCTGCTTTAGCGCTTCCATTACATCCTTTGCAATTCCTTCCCCATTCT20.10172939979654119No Hit
GTACTCAAACAAAAGAGAGACCAAGAGGTCATGAAAACTGACCAGTTCAT20.10172939979654119No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT20.10172939979654119No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA20.10172939979654119No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA20.10172939979654119No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT20.10172939979654119No Hit
GGGATTGGTTTTGATTTTGTCTGATATTTGAAACATTTTCTTCCCAATGA20.10172939979654119No Hit
GTATCAGTCACACGTAAAAAGTTGAAATGGGAGGATCTAAGACCAATAGG20.10172939979654119No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT20.10172939979654119No Hit
ACAAAAAAGAAGGACCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGT20.10172939979654119No Hit
AGATGGAGAAGGCGAAGCAGAACTAGCAGAAAAATTACACTGTTGGTTCG20.10172939979654119No Hit
CCTATAATGTTTGTACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT20.10172939979654119No Hit
TAATTGTACTACTAATGGTAGTAACATCCAATGCAGATCGAATCTGCACT20.10172939979654119No Hit
GATTATAGGTACAGATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG20.10172939979654119No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACGGGCAGGA20.10172939979654119No Hit
CTTATAGACAGATAGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA20.10172939979654119No Hit
GCAAAAAACAGAAAGGGTTTCCTCATAAAGAGGATACCAATGAAAGTAAA20.10172939979654119No Hit
GTTACAGCCAAACGAGAAGCAGCAGTTGAGTAATAGAGCAGTATAGTATG20.10172939979654119No Hit
GTGTAACGGTTATATCTACAGGCACATTCTATGGTTTTGTTGCTGGCAAA20.10172939979654119No Hit
TAGCAGAAGGCCAAGAAAGCTCAGCGCTACTATATTGTCTCATGGTCATG20.10172939979654119No Hit
TCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGC20.10172939979654119No Hit
GGTTCAAACATTACAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG20.10172939979654119No Hit
ACATAATGTGTGGATACTCCATTAGCAGATGAGGTGAACTTTTGAGGATT20.10172939979654119No Hit
GGAATAAACTAACAGAATCTAGGTCCCAATCGATGATTGTGGCTTGTAGA20.10172939979654119No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA20.10172939979654119No Hit
GGGCAAGTCAGAAGAATGGGGAAGGAATTGCAAAGGATGTAATGGAAGTG20.10172939979654119No Hit
GTTAAAACAGGCCACAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT20.10172939979654119No Hit
ACTTCATATTGTGATCTCAAATTTTGTTCTTTCCCTTGTCCTTCTAATGC20.10172939979654119No Hit
TGTAAGCAGTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCAC20.10172939979654119No Hit
ATTACATCCTTTGAAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA20.10172939979654119No Hit
ATTGCCTACCTGCGTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAACT20.10172939979654119No Hit
GTTTATTGTCTCTGTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC20.10172939979654119No Hit
GATATATCCCTTAATCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC20.10172939979654119No Hit
TCATTATATCTTTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTG20.10172939979654119No Hit
GTTCTATAGAGATAGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT20.10172939979654119No Hit
TCTCTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTA20.10172939979654119No Hit
CTTACAACAACACGAACAAAATCTTATTTTGCAAATCTCAAAGGAACAAG20.10172939979654119No Hit
GGAGTATTGAGATACCTTGGGGCAAGTCAGAAGAATGGGGAAGGAATTGC20.10172939979654119No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA20.10172939979654119No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT20.10172939979654119No Hit
ATAATAAACAGTGAAGACGAGCATCTATTGGCACTTGAGAGAAAACTAAA20.10172939979654119No Hit
CCATTAGGTTGTACTTCAATACTATTTCTGGGATATGTAAGTTTCTCAAA20.10172939979654119No Hit
CTGATATACAGAAGGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC20.10172939979654119No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT20.10172939979654119No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC20.10172939979654119No Hit
GATAAAATAAAAGTACAAATTGAAAGAATCTGGAATGGTTCAAACATTAT20.10172939979654119No Hit
GTATTCCAGCGGTGGCAATCGCTCTTCTTTTTAGTTTGCCCCTTTCAGCA20.10172939979654119No Hit
GGGAAAAGGAGAAAACGTCCAAAAACTGGCAGAAGAACTGCAAAGCAACA20.10172939979654119No Hit
CTTCAATACTATTTCTGGGATATGTAAGTTTCTCAAATTGTAAATGTTGG20.10172939979654119No Hit
GTAGAAACACGAGACTTTTTTCATTTTAATCATTTGTCTATCACATGTAT20.10172939979654119No Hit
ATTACTATGTGGTAACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT20.10172939979654119No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)