FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9105_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9105_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643203
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG77480.47151812648832797No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC52580.3199848101543145No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG51200.3115865781647186No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT48120.2928426980719972No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC42690.2597974808955436No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT41070.24993868682080062No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG37340.22723911774747246No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT34620.2106860807824718No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG32260.1963238869451918No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA31410.19115106289362907No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG30670.18664766313109216No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA30450.18530881455304063No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA29500.17952742296599994No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC28480.1733200341041247No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC27880.1696686288912569No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT27150.16522608588226775No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC26480.16114868339456537No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT26040.1584709862384623No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA24770.15074217853789215No Hit
GTCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC24020.1461779220218074No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT23820.1449607869508515No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA23790.14477821669020807No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG23660.14398707889408674No Hit
GTTCTTATCTCTTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA23330.14197880602700944No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC22990.13990967640638435No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC22810.13881425484252402No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT22330.13589313067222977No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT21910.13333714702322233No Hit
CTCATAGACAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC21890.13321543351612675No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT21770.13248515247355316No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA21690.1319982984451708No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC20750.12627776361167795No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA20540.1249997717871742No Hit
GATAATGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT20370.12396520697686166No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG20360.12390435022331385No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA19900.12110493956011521No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA19590.11921838020013352No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA19560.11903580993949013No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG19480.11854895591110776No Hit
CTCATTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC19280.11733182084015183No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA19140.11647982629048267No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG18770.1142281264092142No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC18490.1125241373098759No Hit
CTCTTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT18380.11185471302085012No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA18150.11045500768925082No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT18140.11039415093570301No Hit
CTATTAGAAGGGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG17940.10917701586474707No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAAC17880.1088118753434603No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT17860.10869016183636471No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC17800.10832502131507794No Hit
TCCCTGTACACATTGTGGTCATAAGTTTCATTTCTTATTGATTCTATGCA17090.10400419181318438No Hit
AAACAAGGTAGTTTTTTACTCCAATTCTATGCTGACAAAATGACTGTCGT16970.10327391077061081No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGAAATCTAATGTCGCA16870.10266534323513285No Hit
GTTCAAGAGTGCTTGATCCCATATTATCCATGTTCTCATTTGAAGCAATT16680.10150906491772471No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT16520.10053535686095996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCC403.274181E-11126.939156145
AGCGTAA650.0122.75435145
ACCGACC404.800313E-9108.80499145
CAGGGGG259.368993E-487.044145
GCTGTCC1000.087.044145
TCACGCC750.087.04399145
TGACGCC750.087.04399145
AGACGCC608.276402E-1084.62611145
TATATTC3350.084.327463
ACCGAAA353.4293254E-582.89904145
TTATATT3350.082.162712
GCTGACC1950.081.83623145
ATATTCA3800.076.2498254
CGAAAGC3750.073.4009253
GCTACAA300.001932085572.53666145
AGCGAAA3700.072.430711
GAGCGAA1550.070.094241
GGTGGCC451.1911023E-464.477036145
GGGACCC350.003559774762.174282145
TGGGGCC350.003559774762.174282145
Produced by FastQC (version 0.11.5)