FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9088_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9088_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246723
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA135300.6022104193529865No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT117590.5233845026734493No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT112410.5003287009569048No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT103510.4607154509033824No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT99240.44170999273163625No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA98070.4365024081740384No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC82030.3651095395382519No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC80200.35696434317893216No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG75430.3357334215210331No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG74680.3323952262918037No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT74590.33199464286429614No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA71740.31930950099322436No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT70890.31552621306676437No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA65070.28962181808794407No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA64920.2889541790420982No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT58640.26100235765601726No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT54230.24137376970814828No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA53490.2380800837486419No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT53470.2379910652091958No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT53460.23794655593947275No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT52360.23305053626993624No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC51310.22837706294901505No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT50280.22379260816754004No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC49650.22098852417498732No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG47970.2135109668615134No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG47440.21115197556619128No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA47090.20959415112588423No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT46550.20719065056083902No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC45240.20135993622711837No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT45190.20113738987850308No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC44890.1998021117868113No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA44640.1986893800437348No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG43420.19325924913752163No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC41560.18498052496903267No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA41050.18271055221315669No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA40950.18226545951592607No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA40190.17888275501697362No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG39450.17558906905746724No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT38690.1722063645585148No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT37560.16717681707980914No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA37350.1662421224156249No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG36070.16054493589107335No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG34530.15369050835372228No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC34390.15306737857759947No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT34160.1520436653739691No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA33600.1495511462694778No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC32980.14679157154664815No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG32500.14465512659994134No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG32500.14465512659994134No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT32200.14331984850824958No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC31080.13833481029926697No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA30760.1369105136681291No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC30700.13664345804979075No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT30360.13513014287920674No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA30060.13379486478751496No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT30030.13366133697834578No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA29360.13067921590690085No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT29210.13001157686105497No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA29100.1295219748941013No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG28980.12898786365742462No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC28730.12787513191434816No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG28610.12734102067767145No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC28180.1254271220795799No Hit
CCTTTTCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT27960.1244479181456726No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA27910.12422537179705732No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG27740.1234687142117653No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAA27710.12333518640259614No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT27620.12293460297508861No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT27490.12235598246868884No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT27460.12222245465951967No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA27300.12151030634395073No Hit
CTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGA27280.1214212878045046No Hit
CCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA27200.12106521364672013No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC27110.12066463021921259No Hit
GTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTG27080.12053110241004343No Hit
CTCCTGGCACTGAGGATCTCGCTGCTCTGCTATGAGCTCTGTGTGAATGT27030.12030855606142814No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT27000.12017502825225897No Hit
GCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTC26770.1191513150486286No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT26700.11883975016056718No Hit
CATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGG26180.11652526813496812No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT26040.1159021383588453No Hit
GTTTTTGCTGTGTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCC25900.11527900858272248No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC25130.11185179481404695No Hit
CCTTTATTCTTATTTTCATGTTTACTCTTCTTTTTATTTGATTTAAATGT24980.11118415576820108No Hit
AGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC24880.11073906307097048No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT24250.10793497907841776No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA23950.106599700986726No Hit
CCCAAAGACCAGGAAAGAAAAAGAAGATTCATCACAGAGCCCTTATCGGG23560.1048638394675267No Hit
GCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGGATCTCGC23350.10392914480334248No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA23330.10384012626389635No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG23270.10357307064555799No Hit
ACTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACA22900.10192622766580482No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA22790.10143662569885117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCC100.007076864145.09987145
GCGAGAA600.0120.91654145
CGCACCC301.097651E-7120.91654145
AGCACGC14450.0117.302671
GAGCAGA2050.0116.605751
CACGCAC14600.0116.102683
GCACGCA14600.0116.09752
GGCACCC458.367351E-11112.855446145
GACGGGG203.8561152E-4108.8249145
AAACACG5600.0106.0777745
GAAACAC5600.0106.073054
AGCCACC501.9463187E-10101.5699145
GCACCCC301.5944352E-596.73324145
AACGGCC451.0870281E-896.73324145
ATAAGCC750.096.73324145
AACACGT2050.095.417456
ACACGTG2150.090.979427
TCTCGGG406.0949424E-790.687416145
GCCTCCC406.0949424E-790.687416145
TGACGCC2000.090.68741145
Produced by FastQC (version 0.11.5)