FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_9060_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9060_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003861
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC102450.5112630067654393No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC95320.4756816964849358No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA80760.4030219660944546No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA79490.3966842011496805No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG76520.38186281383788595No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT63880.3187845863560397No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC63560.3171876692046005No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT62350.3111493262257212No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT58850.2936830448818556No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA58540.2921360313913989No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT56610.28250462482178157No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA51450.2567543357548253No Hit
ACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA50330.2511651257247883No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC49660.24782158043896257No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG49410.24657398891440074No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC48750.2432803472895575No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA47650.23779094458148542No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG47380.23644354573495868No Hit
CAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAA46400.23155298695867627No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG46320.2311537576708165No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC46070.22990616614625464No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG45460.22686204282632377No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG45320.22616339157256918No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT45240.22576416228470936No Hit
TCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACC43900.21907707171305796No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT42060.20989479809228284No Hit
CCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG41950.20934585782147563No Hit
CTTCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACA38980.19452447050968105No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT38370.19148034718975018No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38220.19073179227501308No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA37760.1884362238698193No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA36260.1809506747224483No Hit
CCCTTATTCCCATGGAACGGGAACAGGCTACACAATAGACACCGTGATCA35960.17945356489297412No Hit
CACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCTTTGACATTCTTCCT34000.16967244734040934No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT33780.16857456679879493No Hit
GCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC32940.16438265927626716No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA31890.15914277487310746No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC30730.15335395019914058No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT29870.14906223535464785No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA29280.14611791935668192No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT29230.14586840105176957No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG27980.1396304434289604No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC27960.13953063610699545No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG27670.13808342993850373No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT27530.13738477868474908No Hit
GAACACATGAGTACTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA27410.13678593475295941No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG27360.13653641644804704No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT27330.13638670546509962No Hit
TTCCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTA27230.1358876688552749No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA27020.13483969197464296No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT26570.13259402723043165No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA25840.1289510599787111No Hit
GATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGT25830.12890115631772864No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT25830.12890115631772864No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC25700.12825240872495647No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG25010.1248090561171658No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA24640.12296262066081431No Hit
GTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCA24600.1227630060168844No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA23460.11707398866488246No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA23340.11647514473309276No Hit
GTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT22920.11437919097182889No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22840.11397996168396911No Hit
TCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTT22760.11358073239610932No Hit
TATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATC22240.11098574202502069No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT21830.10893969192473929No Hit
TATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGC21760.10859036629786198No Hit
TGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCT21410.10684373816347542No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG21400.10679383450249294No Hit
GTCTTCAGGTAATGGCCCATTTGTTGGATCTATCATTGTACACCCTGTGA21170.10564605029989606No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG21090.10524682101203627No Hit
ATTGTACACCCTGTGATGTCAGAAACATACTGTTTTCCTTTGTTCGAGTA21060.10509711002908884No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT20900.10429865145336926No Hit
GAATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAA20840.10399922948747445No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC20730.10345028921666723No Hit
GAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAA20730.10345028921666723No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT20590.10275163796291259No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACAAAAAATCTCAATT20570.10265183064094766No Hit
CTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTT20400.1018034684042456No Hit
GGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGG20400.1018034684042456No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA20400.1018034684042456No Hit
TTCTTAATCTATGGGCCATAGTGCCGTTGCAGCAGGCTGGTTTCTGCATA20390.10175356474326314No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT20320.10140423911638582No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA20220.1009052025065611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGGG151.2264814E-4145.10988145
CTCGTCT100.0071092388144.878134
ACTCGTC100.0071102995144.87093
CGCACCC500.0130.59889145
GGCACCC850.0128.03812145
AGCACGC6650.0118.7228551
GCACGCA6750.0118.037062
CACGCAC6800.0117.174983
GCTGCCC650.0111.62297145
GAGCAGA2100.0110.3723141
ACTCGCC203.8549947E-4108.832405145
GCAGCCC750.0106.41391145
ATAAGCC1600.0104.29772145
AAACACG11450.0101.8600855
GAAACAC11550.0100.975664
CAGCGCC607.2759576E-1296.73992145
GTACTCC900.096.73992145
CTTAGGG451.0864824E-896.73992145
CCTTAAA850.093.89462145
AACACGT4050.091.226396
Produced by FastQC (version 0.11.5)