FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_9060_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_9060_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011319
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC102640.5103118898593411No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC95630.47545913900281356No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA81000.40272080162321344No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA79700.3962573813502483No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG76700.38134179610494406No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT64060.318497463604729No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC63730.3168567492277456No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT62490.3106916406596865No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT58940.2930415314527432No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA58680.29174884739815016No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT56810.2824514659285772No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA51570.2563989103667792No Hit
ACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA50430.2507309879735636No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC49780.24749927783708106No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG49560.2464054682524254No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC48920.24322347673342717No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA47870.2380030218975707No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG47490.23611371443316548No Hit
CAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAA46500.23119157130221513No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG46450.23094297821479337No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC46160.2295011383077473No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG45540.22641858402371778No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG45440.2259213978488743No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT45380.22562308614396823No Hit
TCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACC44010.2188116355486126No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT42140.20951425407903967No Hit
CCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG42060.2091165051391649No Hit
CTTCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACA39020.1940020454239233No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT38440.19111836560983117No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38330.19057146081750334No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA37840.18813524856077032No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA36370.18082661179057125No Hit
CCCTTATTCCCATGGAACGGGAACAGGCTACACAATAGACACCGTGATCA36080.1793847718835252No Hit
CACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCTTTGACATTCTTCCT34110.16959020423910875No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT33820.1681483643320627No Hit
GCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC33030.16422059355079927No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA31980.15900013871494278No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC30840.1533322163217272No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT29970.14900669660058896No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA29400.14617273540398118No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT29310.14572526784662204No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG28070.139560159278563No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC28020.13931156619114124No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG27780.1381183193715169No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT27580.13712394702182995No Hit
GAACACATGAGTACTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA27450.13647760499453343No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG27390.13617929328962736No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT27380.136129574672143No Hit
TTCCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTA27310.13578154434975256No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA27090.13468773476509693No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT26630.13240067836081695No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA25920.1288706565194283No Hit
GATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGT25870.12862206343200658No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT25870.12862206343200658No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC25770.1281248772571631No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG25050.12454513679829007No Hit
GTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCA24650.1225563920989162No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA24640.12250667348143185No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA23550.11708734417563797No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA23370.11619240906091971No Hit
GTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT22960.11415394574406149No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22940.1140545085090928No Hit
TCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTT22780.11325901062934324No Hit
TATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATC22280.11077307975512586No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT21940.10908264676065807No Hit
TATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGC21780.1082871488809085No Hit
TGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCT21470.10674587173889374No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG21450.10664643450392505No Hit
GTCTTCAGGTAATGGCCCATTTGTTGGATCTATCATTGTACACCCTGTGA21190.105353750449332No Hit
ATTGTACACCCTGTGATGTCAGAAACATACTGTTTTCCTTTGTTCGAGTA21120.10500572012694157No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG21110.10495600150945722No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT20960.10421022224719202No Hit
GAATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAA20890.10386219192480158No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC20840.10361359883737985No Hit
GAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAA20760.10321584989750508No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT20650.10266894510517724No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACAAAAAATCTCAATT20590.10237063340027117No Hit
CTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTT20470.101774009990459No Hit
GGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGG20460.10172429137297465No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA20450.1016745727554903No Hit
TTCTTAATCTATGGGCCATAGTGCCGTTGCAGCAGGCTGGTTTCTGCATA20430.1015751355205216No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT20350.10117738658064683No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA20280.1008293562582564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC351.893568E-9124.38149145
ACTCGCC301.0970689E-7120.926445145
ATAACGG301.0970689E-7120.926445145
GGCACCC850.0119.503784145
AGCACGC5550.0113.541131
GCACGCA5700.0110.555952
GGCAGGG203.8548E-4108.8338145
GTCGCCC203.8548E-4108.8338145
GAGTCCC203.8548E-4108.8338145
GAAACAC11950.0108.498284
AAACACG12100.0107.161265
CACGCAC5900.0106.8083043
GAGCAGA2200.0102.062451
ATAAGCC1450.0100.07706145
GCAGCCC950.099.28698145
AACACGA9350.098.39996
CAGCGCC451.0864824E-896.74116145
AACGGCC451.0864824E-896.74116145
CGATAAA607.2759576E-1296.74116145
AACACGT3900.096.5920266
Produced by FastQC (version 0.11.5)