FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_895_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_895_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946951
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT145980.7497877450434037No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA91960.4723282712302467No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA69260.35573571189002706No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT62700.3220420031115318No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT62250.3197306968691046No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT57930.29754215694180286No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT55190.28346887004346794No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT51970.2669301898198773No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC48800.25064832140100085No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA44910.2306683629942407No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG44730.22974384049726984No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG44310.22758662133767105No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC44140.22671346120164299No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT43550.22368308190601613No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT40400.2075039382090253No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC40270.20683622751676853No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT39800.20442219655245564No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38520.1978478143517736No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT38420.19733419074234532No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT37820.19425244908577566No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA37100.19055435909789203No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA34980.17966553857801248No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT34780.17863829135915593No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC34750.17848420427632744No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT32960.16929034166756124No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT30370.15598749018336877No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA30310.1556793160177118No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC30280.1555252289348833No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA29420.1511080658938001No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC29340.1506971670062575No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG28110.14437959661028962No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG27090.13914063579412117No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA26550.13636706830320844No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC25620.13159036873552546No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT25560.13128219456986848No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG25340.13015222262912626No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC24340.12501598653484347No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT24300.12481053709107214No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT24250.124553725286358No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT24170.12414282639881538No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG23310.11972566335773217No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG22910.11767116892001904No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT22800.11710618294964795No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC22690.11654119697927683No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT22210.11407580365402109No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG21880.11238084574290774No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT21760.11176449741159382No Hit
AAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACT21750.11171313505065099No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA21600.11094269963650856No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA21480.11032635130519464No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA21370.10976136533482352No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA20970.10770687089711041No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT20890.10729597200956778No Hit
CAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGA20600.10580646354222577No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG20590.10575510118128295No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA20480.10519011521091183No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAATTGAATTT20310.10431695507488377No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA20150.10349515729979851No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA20150.10349515729979851No Hit
GTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTC19620.10077295216982861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACCC100.0070776646145.09389145
GACCGGT100.007106549144.896367
TCGCAGT100.007108732144.881474
AGCACGC20100.0135.504071
CACGCAC20250.0134.142623
GCACGCA20200.0134.116072
CGCACCC351.895387E-9124.36617145
CGGTGCA501.8189894E-12115.899232
GAAACAC4750.0103.7046364
CGTTACC352.7521128E-7103.63848145
AAACACG4950.0100.980585
ACGCACT28350.097.609744
CGCACTT28500.097.09855
GGCACCC750.096.729256145
GAGCAGA1400.093.133311
CAGCGCC800.090.68368145
GGAATCC259.3639846E-487.05633145
CGGCACC259.3639846E-487.05633145
CTTGGCC1000.087.05633145
AACGGCC259.3639846E-487.05633145
Produced by FastQC (version 0.11.5)