FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_822_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_822_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202164
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT86490.39275004041479195No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC85070.38630183764696907No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG78230.35524148065266714No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT68350.3103765205497865No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA61060.27727271901638567No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG55620.25256974503261337No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC53490.24289744088087897No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT52950.24044530743396042No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT51450.23363382563696436No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG49020.22259922512583075No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG46310.21029314801259127No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC45750.2077501948083794No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA42660.19371854230656754No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT41010.1862259123298719No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG40140.18227525288761418No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC39360.17873328235317623No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG37430.16996917577437465No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT37380.16974212638114144No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC37320.16946966710926162No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA37160.16874310905091536No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC36100.1639296619143715No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC35930.16315769397737862No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA35250.16006982222940708No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT34380.15611916278714938No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTATGTCCCCCTGC34380.15611916278714938No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT34060.15466604667045686No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT33850.15371243921887742No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT33490.15207768358759838No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA33370.1515327650438387No Hit
GTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC32440.14730964632970114No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA32010.14535702154789562No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT30640.1391358681733059No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT30610.13899963853736597No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA30360.13786439157119995No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA29850.1355484877602213No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCA29850.1355484877602213No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC29200.1325968456481897No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT29150.13236979625495648No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA29080.13205192710443No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGATCCAGC29010.13173405795390353No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT29000.13168864807525688No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA28750.13055340110909086No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC28530.12955438377886477No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT28290.1284645466913454No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA27130.12319700076833516No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT26790.12165306489434938No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA26500.1203361784135968No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC26450.12010912902036361No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA26230.11911011169013752No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT26010.11811109435991143No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG25870.11747535605885846No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT25760.11697584739374543No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG25230.11456912382547349No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT25090.11393338552442053No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG25040.11370633613118732No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC25040.11370633613118732No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG25020.11361551637389404No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC24800.11261649904366795No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGAT24790.11257108916502132No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA24690.11211699037855492No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG24400.11080010389780234No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT24280.11025518535404265No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC24130.10957403717434305No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG24070.1093015779024632No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA23920.1086204297227636No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC23790.1080301013003573No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC23720.1077122321498308No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG23640.10734895312065768No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA23550.10694026421283792No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAAATTGCAACACTTCT23520.106804034576898No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT23440.10644075554772486No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC23420.10634993579043159No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT23210.10539632833885214No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC23060.10471518015915254No Hit
GATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGT23000.10444272088727269No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG22860.10380698258621975No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGACTCCAGC22660.10289878501328693No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG22640.10280796525599366No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG22630.102762555377347No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG22240.10099157011012805No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC22210.10085534047418812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC2000.0119.72065145
TCACGCC850.0119.50723145
ACCGCCC501.9463187E-10101.581154145
AGTAGAA11200.098.9483261
CGATTAA451.0863005E-896.74395145
TGACGCC850.093.89854145
GAGCGAA850.093.736511
GCGAAAG6750.091.215772
CCTTACC1050.089.833664145
CCTCGCC900.088.68195145
CGAAAGC7050.087.336233
CTCACCC259.358496E-487.06956145
GTCGACC259.358496E-487.06956145
CGGACCC850.085.36231145
GGCAGCC554.3726686E-879.154144145
GCTGGCC851.8189894E-1276.82608145
ACAACGA300.00192992472.55796145
ACACCCC903.6379788E-1272.55796145
GTAGAAA21750.070.6011351
AGCGTAA754.827598E-967.720764145
Produced by FastQC (version 0.11.5)