FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_709_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_709_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567159
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA40740.2599608591087439No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA40340.25740846972132375No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT37080.2366064962138494No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT32280.2059778235648074No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA32070.20463781913641182No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT31790.20285114656521772No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC28510.18192155358837234No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT25820.16475673495797172No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC25740.16424625708048768No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG25600.1633529207948906No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT25060.1599071951218734No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA24940.15914147830564737No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC24920.15901385883627636No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA24690.15754623493850975No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA24560.15671670838759819No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24420.15582337210200112No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC24140.154036699530807No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA24110.1538452703267505No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC23390.14925096942939423No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT22910.14618810216449No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA22830.14567762428700598No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA22800.14548619508294947No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAACTTGAGAAACTTACATA22710.14491190747077992No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC22520.14369952251175536No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC22210.14172142073650473No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG22210.14172142073650473No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC21640.13808426585943098No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT21450.13687188090040642No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG20110.12832137645254885No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG19910.12704518175883878No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19860.12672613308541125No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT19470.12423755343267658No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC19450.12410993396330557No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC19180.12238707112679696No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT19170.12232326139211146No Hit
TTCTTAATCTATGGGTCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT18900.12060039855560285No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT18640.11894134545377974No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA18510.11811181890286819No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA18500.11804800916818269No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG18300.1167718144744726No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT17730.11313465959739885No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA17610.1123689427811728No Hit
TTATAAAGGAGCTATGGGAAAACACTAAAGGAAGAGATGGTCTGTTAGTA17580.11217751357711629No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA17510.11173084543431777No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC17470.11147560649557575No Hit
CATCAAAAGAGCATTGTCATTAAACACAATGACAAAAGATGCTGAAAGGG17380.11090131888340622No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC16960.10822131002661504No Hit
TATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGT16850.1075194029450745No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC16400.1046479648842268No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG16380.10452034541485579No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT16160.10311653125177471No Hit
GTTCTACAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16110.10279748257834719No Hit
GTTCATAGCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCA16110.10279748257834719No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC16090.10266986310897618No Hit
ATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACA16010.10215938523149215No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGGATCTTTTTGGCTATGACT16000.10209557549680665No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC15760.10056414186435454No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA15740.10043652239498353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG10000.099.943115
GCAATCC750.096.7579145
TAGAAAC10800.096.5510562
CCCGACC406.084829E-790.710526145
AACACGA7650.085.211146
GAAACAC12600.079.884514
GCGAGAA554.3673936E-879.16555145
CAACGCA2150.077.490119
ACGAGCC3100.072.436419
AACAACG2350.070.8906867
GCCGACC851.6916601E-1068.2997145
TCCGCCC451.1885067E-464.50527145
CCGGGGG808.030838E-963.497368145
ACGTGCC1500.062.778229
GTCGCCC702.320212E-762.201508145
GAACGCC954.5292836E-1061.110252145
GGATACC1550.060.7531179
CGTCTCC606.7694164E-660.473686145
AACGGCC606.7694164E-660.473686145
ACACGTG6600.059.2623677
Produced by FastQC (version 0.11.5)