FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_709_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_709_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574441
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA40880.2596477098856039No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA40510.25729766945855703No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT37200.2362743348274086No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT32370.2055967800635273No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA32190.2044535171530721No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT31870.20242104975670727No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC28550.18133420051942245No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT25850.16418525686259441No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC25810.1639311984380488No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG25670.1630419939521392No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT25130.1596122052207736No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA25030.1589770591594096No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC25010.1588500299471368No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA24750.15719865018759038No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA24670.1566905333384992No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24510.1556742996403168No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC24230.15389589066849757No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA24140.15332425921326998No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC23440.14887823678372197No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT22960.14582953568917476No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA22910.14551196265849276No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA22890.14538493344621997No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAACTTGAGAAACTTACATA22810.14487681659712875No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC22600.14354300986826435No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG22310.14170108629030875No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC22280.14151054247189954No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC21730.13801723913439756No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT21480.13642937398098753No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG20160.12804544597098272No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG19980.1269021830605275No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19890.1263305516052999No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC19550.12417105499666231No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT19500.12385348196598031No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT19240.1222021022064339No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC19210.1220115583880247No Hit
TTCTTAATCTATGGGTCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT18950.12036017862847831No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT18660.1185182550505227No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA18600.1181371674137043No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA18560.1178831089891587No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG18390.1168033606848399No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT17780.11292896971051948No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA17660.11216679443688268No Hit
TTATAAAGGAGCTATGGGAAAACACTAAAGGAAGAGATGGTCTGTTAGTA17630.11197625061847348No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA17570.11159516298165507No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC17530.11134110455710948No Hit
CATCAAAAGAGCATTGTCATTAAACACAATGACAAAAGATGCTGAAAGGG17480.11102353152642748No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC16990.10791131582574387No Hit
TATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGT16870.10714914055210706No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG16450.10448152709437826No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC16430.10435449788210546No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT16230.10308420575937745No Hit
GTTCTACAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16140.10251257430414985No Hit
GTTCATAGCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCA16140.10251257430414985No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC16120.10238554509187706No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGGATCTTTTTGGCTATGACT16080.10213148666733145No Hit
ATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACA16040.10187742824278585No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC15780.10022604848323946No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA15770.10016253387710304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG8700.095.741715
CGACGAA1850.094.1076145
TAGAAAC9700.092.588872
AACGGCC554.1472958E-1092.32526145
GCCGACC1150.088.31111145
AACACGA6550.082.941176
ACGAGCC2400.078.476549
ACGTGCC1300.078.0121849
GAAACAC11250.073.396424
GTCGCCC406.6456094E-572.541275145
CACGACC809.822543E-1172.541275145
ATAAGCC809.822543E-1172.541275145
CGCCGAA502.2990698E-672.54127145
TAGTCCC300.001931583672.54127145
GCAATCC1250.069.63962145
CGATAAA754.834874E-967.705185145
CAACGCA2050.067.139419
ACGCACT6150.061.2422074
AACAACG2250.061.1695187
CGCACTT6100.060.5568055
Produced by FastQC (version 0.11.5)