FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_703_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_703_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013464
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTG79710.786510423655897No Hit
ATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAG79360.783056921607477No Hit
CTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACA57270.5650916066086216No Hit
GTAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCC55710.5496988546213778No Hit
AAACTATAATGCGCAAATTCTACTCTGCTTGTAATACACACTGTATGCTA41620.4106707293006954No Hit
GATATAGACAGAGCAGAGAGAGGCGCCAAGCAATGGAATGAAGAAGGGAG38870.3835360703488235No Hit
ATTCTACTCTGCTTGTAATACACACTGTATGCTAGCCTATTTACCCGTAA37500.37001807661643626No Hit
GGTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTT36430.35946022749698064No Hit
ATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAGTA33130.32689863675473424No Hit
ATATAAACTAATGCCGTCATTCACATAGTTTTCTGCTTCATTGCGCTGCT32070.3164394591223763No Hit
AATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGT26070.2572365668637465No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC23560.23247002360221974No Hit
GCTTGTAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAA22720.22418161868601152No Hit
CTCTTATTCAGTCTCTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20980.2070127799310089No Hit
CTATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAATGACG19040.18787051143405192No Hit
TCCTTAAGGAGAACATTAACCGATCAGGGGTAGAAGCCACCTCTTGCCTT18800.18550239574370672No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT17020.16793887104031321No Hit
GCTTACAGCTGCACATTCCCACCAAACTATAATGCGCAAATTCTACTCTG17010.16784019955321552No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT16640.1641893545306No Hit
ATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAATGACGGC16430.16211725330154797No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16200.1598478090983005No Hit
GAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAGTAGGTTTACAGT16100.15886109422732334No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT15870.15659165002407585No Hit
GTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACATA15850.15639430704988042No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA15220.15017800336272427No Hit
CCATTAAAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG14830.14632981536591333No Hit
GGCTAATAGATTTCTTAAAGGATGTGATGGAATCAATGAACAAAGAGGAA14070.1388307823464869No Hit
GTACTGGAAGGGACCTGCAAGCTGTAGAGAAAACACAAGGAAGGGTAAGA14060.1387321108593892No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA14050.13863343937229147No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG13580.1339958794786988No Hit
ATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACATAGT13440.13261447865933076No Hit
TATTAGCACTCATATGGCATATGGACATGTTGCATACATGATTCCTTGAA13420.13241713568513536No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT13350.1317264352754513No Hit
TTGTAGAACCGCTTAGTAGGTTTACAGTACACTATTATAAGGATAAGCAG13320.13143042081415818No Hit
AAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTT13170.1299503485076924No Hit
TTATAATAGTGTACTGTAAACCTACTAAGCGGTTCTACAACCAATACTTT12570.12403005928182945No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTCTGT12470.1230433444108523No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC12420.1225499869753637No Hit
CGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACC12170.1200831997979208No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAAATTGCAACACTTCT12000.11840578451725962No Hit
ACTATAATGCGCAAATTCTACTCTGCTTGTAATACACACTGTATGCTAGC11950.11791242708177105No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC11630.1147549394946441No Hit
CATTAAAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA11420.11268283826559207No Hit
CCTTATGACTCACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA11420.11268283826559207No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC11200.1105120655494423No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAAACGAGAAGATCATTT11140.109920036626856No Hit
CCATAGAAGTTTTTAGATCGAATGACCTAACAGCTAACGAGTCAGGAAGG11090.10942667919136743No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGATCCAGC10990.10843996432039027No Hit
AAATAAGGTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTA10940.10794660688490168No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG10890.10745324944941309No Hit
CCCATAATGTTCTCAAATAAAATGGCAAGACTAGGAAAAGGGTACATGTT10850.10705856350102223No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT10650.10508513375906792No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG10580.10439443334938389No Hit
TAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCG10540.10399974740099302No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT10510.10370373293969988No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC10350.10212498914613642No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT10340.1020263176590387No Hit
GTACACTATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAA10300.10163163171064785No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG10290.10153296022355011No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCCATTAAAAGAGGCCTTATTTTC10280.1014342887364524No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC10240.10103960278806154No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA10220.10084225981386612No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCC100.00707814145.0872145
ACCGTCG100.0071032634144.915378
TTAGGAA203.856835E-4108.81541145
AGCGTTC203.875061E-4108.686538
GCGTTCC203.875061E-4108.686539
GTAAGCC451.08721E-896.72481145
CTAGACG700.093.146094
GTTCCCC406.09567E-790.679504145
TACGGGG259.364388E-487.05233145
TCGGTCC259.364388E-487.05233145
CGGACCC259.364388E-487.05233145
ATAGGGT1500.086.936354
GTCCTAG750.086.927771
TCCTAGA750.086.927772
TAGGGTG1750.082.800615
CGAAAGC6000.080.895123
ACAAGGC1100.079.044758
GCATAGG1700.076.700982
AGTTTCC300.001931137672.5436145
TGACGCC607.99846E-872.5436145
Produced by FastQC (version 0.11.5)