FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_703_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_703_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016675
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTG79790.7848132392357439No Hit
ATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAG79570.7826493225465365No Hit
CTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACA57350.5640937369365825No Hit
GTAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCC55770.5485528807140925No Hit
AAACTATAATGCGCAAATTCTACTCTGCTTGTAATACACACTGTATGCTA41740.41055401185236184No Hit
GATATAGACAGAGCAGAGAGAGGCGCCAAGCAATGGAATGAAGAAGGGAG39030.38389849263530623No Hit
ATTCTACTCTGCTTGTAATACACACTGTATGCTAGCCTATTTACCCGTAA37580.3696363144564389No Hit
GGTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTT36490.35891509085991097No Hit
ATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAGTA33210.32665306022081786No Hit
ATATAAACTAATGCCGTCATTCACATAGTTTTCTGCTTCATTGCGCTGCT32130.3160301964737994No Hit
AATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGT26140.25711264661765065No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC23600.2321292448422554No Hit
GCTTGTAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAA22780.22406373718248213No Hit
CTCTTATTCAGTCTCTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20990.2064573241202941No Hit
CTATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAATGACG19120.18806403226203064No Hit
TCCTTAAGGAGAACATTAACCGATCAGGGGTAGAAGCCACCTCTTGCCTT18830.18521159662625716No Hit
GCTTACAGCTGCACATTCCCACCAAACTATAATGCGCAAATTCTACTCTG17050.16770354341357857No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT17030.16750682371455972No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT16700.1642609486807485No Hit
ATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAATGACGGC16490.16219539184105047No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16240.15973639560331473No Hit
GAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAGTAGGTTTACAGT16130.15865443725871098No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT15910.15649052056950352No Hit
GTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACATA15890.15629380087048467No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA15230.14980205080286227No Hit
CCATTAAAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG14840.1459660166719945No Hit
GGCTAATAGATTTCTTAAAGGATGTGATGGAATCAATGAACAAAGAGGAA14090.13858902795878722No Hit
GTACTGGAAGGGACCTGCAAGCTGTAGAGAAAACACAAGGAAGGGTAAGA14090.13858902795878722No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA14080.1384906681092778No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG13620.133966115031844No Hit
ATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACATAGT13510.13288415668724027No Hit
TATTAGCACTCATATGGCATATGGACATGTTGCATACATGATTCCTTGAA13490.1326874369882214No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT13390.1317038384931271No Hit
TTGTAGAACCGCTTAGTAGGTTTACAGTACACTATTATAAGGATAAGCAG13330.13111367939607052No Hit
AAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTT13190.1297366415029385No Hit
TTATAATAGTGTACTGTAAACCTACTAAGCGGTTCTACAACCAATACTTT12590.12383505053237269No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTCTGT12480.12275309218776895No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC12420.12216293309071237No Hit
CGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACC12180.11980229670248606No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAAATTGCAACACTTCT12040.11842525880935402No Hit
ACTATAATGCGCAAATTCTACTCTGCTTGTAATACACACTGTATGCTAGC11950.11754002016376915No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC11640.1144908648289768No Hit
CATTAAAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA11450.11262202768829764No Hit
CCTTATGACTCACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA11420.11232694813976934No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC11230.11045811099909017No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAAACGAGAAGATCATTT11150.10967123220301472No Hit
CCATAGAAGTTTTTAGATCGAATGACCTAACAGCTAACGAGTCAGGAAGG11110.10927779280497701No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGATCCAGC11020.10839255415939213No Hit
AAATAAGGTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTA10980.10799911476135442No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG10930.10750731551380727No Hit
CCCATAATGTTCTCAAATAAAATGGCAAGACTAGGAAAAGGGTACATGTT10860.10681879656724125No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT10680.1050483192760715No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG10610.1043598003295055No Hit
TAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCG10560.10386800108195834No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT10510.1033762018344112No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT10370.10199916394127917No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC10350.10180244424226031No Hit
GTACACTATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAA10340.10170408439275087No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG10310.10140900484422259No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCCATTAAAAGAGGCCTTATTTTC10300.10131064499471316No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA10280.1011139252956943No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC10250.10081884574716601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCC253.6945494E-8145.11139145
CGGTTTT100.007074619145.11137145
AGCAGTC100.007104879144.904378
AGCGGGG203.8542834E-4108.833534145
CTCGAAA203.8542834E-4108.833534145
CGAAAGC6750.0100.876533
TACGGGG451.0859367E-896.74092145
ATAGGGT1200.096.588664
GATATAG7550.087.3092961
GTACCCC259.3582005E-487.06683145
TAGGGTG1500.086.938355
GCATAGG1350.085.852372
TCTGACC608.240022E-1084.6483145
ATATAGA7850.083.972632
ACTGACC353.424236E-582.92079145
CGGGCTG353.424236E-582.92079145
ATAGACA8000.082.40224
TATAGAC8100.081.380893
ACAGAGC8550.077.112868
TGACGCC950.076.374405145
Produced by FastQC (version 0.11.5)