FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_581_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_581_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314221
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20200.6428596433720216No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC18640.5932130570522021No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG17010.5413387392949548No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT14890.47387030147571296No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA14610.4649593757260017No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT12130.38603403337141695No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG12020.38253331254117323No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC11250.3580282667294675No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTATGC10980.34943558832796023No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT10600.3373421890962094No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG9420.29978900200814074No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG9150.29119632360663356No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC8780.2794211717230866No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT8750.2784664296784747No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC8730.27782993498206676No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT8390.26700952514313175No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA8310.26446354635749997No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT7930.25237014712574907No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT7720.24568695281346567No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA7660.24377746872424186No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT7450.23709427441195846No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA7250.23072932744787905No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG7050.2243643804837996No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT6930.22054541230535196No Hit
CTTTCAATCTATGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT6920.220227164957148No Hit
AAACTATTCTCTGTCGCTTTCAATCTATGCCGTATTTCTTCAATTAACCA6820.2170446914751083No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA6760.21513520738588443No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT6620.21067974451102886No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC6470.2059060342879693No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT6230.19826809793107397No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC6210.19763160323466605No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC5980.1903119142259747No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC5920.1884024301367509No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT5810.1849017093065072No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT5770.1836287199136913No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG5710.18171923582446747No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA5640.1794915043870397No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC5570.1772637729496119No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC5400.1718535680301444No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG5340.16994408394092056No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG5310.16898934189630865No Hit
ATTCTCTGTCGCTTTCAATCTATGCCGTATTTCTTCAATTAACCACCTTA5250.16707985780708481No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA5240.16676161045888085No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT5140.16357913697684112No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA5130.16326088962863716No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC5080.1616696528876173No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG5030.16007841614659746No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT5030.16007841614659746No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG5030.16007841614659746No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT5020.1597601687983935No Hit
TGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGAC5010.1594419214501895No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC5000.15912367410198555No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4930.15689594266455775No Hit
GTCATGGATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAA4890.15562295327174186No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA4850.154349963878926No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG4810.1530769744861101No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT4770.1518039850932942No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC4640.14766676956664257No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG4510.14352955403999096No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC4500.143211306691787No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT4500.143211306691787No Hit
GATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGT4490.142893059343583No Hit
CATCTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATG4440.14130182260256316No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTC4420.1406653279061552No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA4400.14002883320974727No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT4390.1397105858615433No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA4390.1397105858615433No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTAA4380.13939233851333932No Hit
AAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCTTTC4340.13811934912052345No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTC4330.1378011017723195No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT4310.13716460707591155No Hit
CAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTG4290.1365281123795036No Hit
CTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT4260.1355733703348917No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG4200.13366388624566786No Hit
CATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC4190.1333456388974639No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC4140.13175440215644402No Hit
GTCCCAATTGTTCCCGCCACTTCTCATTTCTGCTCTGGAGGTAGTGAAGG4080.1298449180672202No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA4070.12952667071901625No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT4030.12825368132620035No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT4020.1279354339779964No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG3970.1263441972369765No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG3960.12602594988877255No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT3950.1257077025405686No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT3930.12507120784416065No Hit
GTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT3870.12316172375493681No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC3870.12316172375493681No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT3860.12284347640673283No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGTTACATTTCTGAC3760.11966100292469313No Hit
CGATTGGACCAGGCGGTCATGGATAAGAACATAGTACTGGAAGCAAACTT3760.11966100292469313No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCA3730.11870626088008121No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC3710.11806976618367326No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC3680.11711502413906136No Hit
GTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCAATTGT3680.11711502413906136No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT3670.1167967767908574No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG3660.11647852944265341No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA3650.11616028209444945No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC3650.11616028209444945No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC3630.1155237873980415No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTCTACTTCAA3590.11425079800522563No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC3560.11329605596061372No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT3540.11265956126420577No Hit
ATGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA3510.11170481921959384No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATT3500.11138657187138988No Hit
TGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTATGCCGTATTT3480.11075007717498195No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT3480.11075007717498195No Hit
GTGTAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT3460.110113582478574No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGG3460.110113582478574No Hit
GATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAGA3450.10979533513037004No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACT3450.10979533513037004No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAA3450.10979533513037004No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTATGCCGTATTTCTTCAATT3420.10884059308575811No Hit
GGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAG3410.10852234573755415No Hit
CTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCA3400.10820409838935016No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC3370.10724935634473826No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC3370.10724935634473826No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA3370.10724935634473826No Hit
CATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGA3350.10661286164833031No Hit
CAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGG3340.10629461430012634No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3340.10629461430012634No Hit
GACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAA3310.10533987225551443No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT3290.10470337755910648No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC3290.10470337755910648No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG3270.10406688286269854No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC3220.1024756461216787No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAA3210.10215739877347473No Hit
ACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGA3180.1012026567288628No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT3170.10088440938065885No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATGCC3170.10088440938065885No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATT3160.10056616203245486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGCC151.227799E-4145.04175145
TCACGCC100.007082464145.04173145
GCAGTTT100.007082464145.04173145
CGATTAA100.007082464145.04173145
TGACGCC100.007082464145.04173145
CCTCGCC202.12526E-6145.04173145
ACCTCCC100.007082464145.04173145
ACCACCC100.007082464145.04173145
GCGAAAG950.0114.3790742
CGAAAGC1100.098.781943
ATATTCA406.1377796E-790.55014
AGTAGAA1500.086.928111
GCACCGT851.8189894E-1276.713476
CACCGTC851.8189894E-1276.713477
ACTCAGC851.8189894E-1276.701261
CTCAGCA851.8189894E-1276.701262
GTAGAAA2850.076.2527161
ATTGGGG406.646588E-572.52087145
ACCGTCT903.6379788E-1272.463148
TTAACAG754.829417E-967.68614145
Produced by FastQC (version 0.11.5)